DOI: 10.1083/jcb.201002070. The MIS12 complex is a protein interaction hub for outer kinetochore assembly

The MIS12 complex is a protein interaction hub for outer kinetochore assembly

10.1083/jcb.201002070

Crossref journal-article

Rockefeller University Press

Journal of Cell Biology (291)

Abstract

Kinetochores are nucleoprotein assemblies responsible for the attachment of chromosomes to spindle microtubules during mitosis. The KMN network, a crucial constituent of the outer kinetochore, creates an interface that connects microtubules to centromeric chromatin. The NDC80, MIS12, and KNL1 complexes form the core of the KMN network. We recently reported the structural organization of the human NDC80 complex. In this study, we extend our analysis to the human MIS12 complex and show that it has an elongated structure with a long axis of ∼22 nm. Through biochemical analysis, cross-linking–based methods, and negative-stain electron microscopy, we investigated the reciprocal organization of the subunits of the MIS12 complex and their contacts with the rest of the KMN network. A highlight of our findings is the identification of the NSL1 subunit as a scaffold supporting interactions of the MIS12 complex with the NDC80 and KNL1 complexes. Our analysis has important implications for understanding kinetochore organization in different organisms.

Bibliography

Petrovic, A., Pasqualato, S., Dube, P., Krenn, V., Santaguida, S., Cittaro, D., Monzani, S., Massimiliano, L., Keller, J., Tarricone, A., Maiolica, A., Stark, H., & Musacchio, A. (2010). The MIS12 complex is a protein interaction hub for outer kinetochore assembly. Journal of Cell Biology, 190(5), 835â852.

Authors 13

Arsen Petrovic (first)
Sebastiano Pasqualato (additional)
Prakash Dube (additional)
Veronica Krenn (additional)
Stefano Santaguida (additional)
Davide Cittaro (additional)
Silvia Monzani (additional)
Lucia Massimiliano (additional)
Jenny Keller (additional)
Aldo Tarricone (additional)
Alessio Maiolica (additional)
Holger Stark (additional)
Andrea Musacchio (additional)

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Dates

Type	When
Created	14 years, 11 months ago (Sept. 6, 2010, 10:33 a.m.)
Deposited	2 years, 1 month ago (July 29, 2023, 1:08 a.m.)
Indexed	1 week, 1 day ago (Aug. 23, 2025, 9:51 p.m.)
Issued	14 years, 11 months ago (Sept. 6, 2010)
Published	14 years, 11 months ago (Sept. 6, 2010)
Published Online	14 years, 11 months ago (Sept. 6, 2010)
Published Print	14 years, 11 months ago (Sept. 6, 2010)

Funders 0

None

BibTeX

@article{Petrovic_2010, title={The MIS12 complex is a protein interaction hub for outer kinetochore assembly}, volume={190}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.201002070}, DOI={10.1083/jcb.201002070}, number={5}, journal={Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Petrovic, Arsen and Pasqualato, Sebastiano and Dube, Prakash and Krenn, Veronica and Santaguida, Stefano and Cittaro, Davide and Monzani, Silvia and Massimiliano, Lucia and Keller, Jenny and Tarricone, Aldo and Maiolica, Alessio and Stark, Holger and Musacchio, Andrea}, year={2010}, month=sep, pages={835–852} }

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  "abstract": "<jats:p>Kinetochores are nucleoprotein assemblies responsible for the attachment of chromosomes to spindle microtubules during mitosis. The KMN network, a crucial constituent of the outer kinetochore, creates an interface that connects microtubules to centromeric chromatin. The NDC80, MIS12, and KNL1 complexes form the core of the KMN network. We recently reported the structural organization of the human NDC80 complex. In this study, we extend our analysis to the human MIS12 complex and show that it has an elongated structure with a long axis of \u223c22 nm. Through biochemical analysis, cross-linking\u2013based methods, and negative-stain electron microscopy, we investigated the reciprocal organization of the subunits of the MIS12 complex and their contacts with the rest of the KMN network. A highlight of our findings is the identification of the NSL1 subunit as a scaffold supporting interactions of the MIS12 complex with the NDC80 and KNL1 complexes. Our analysis has important implications for understanding kinetochore organization in different organisms.</jats:p>",
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