DOI: 10.1083/jcb.201001149. Vinculin potentiates E-cadherin mechanosensing and is recruited to actin-anchored sites within adherens junctions in a myosin II

Vinculin potentiates E-cadherin mechanosensing and is recruited to actin-anchored sites within adherens junctions in a myosin II–dependent manner

10.1083/jcb.201001149

Crossref journal-article

Rockefeller University Press

Journal of Cell Biology (291)

Abstract

Cell surface receptors integrate chemical and mechanical cues to regulate a wide range of biological processes. Integrin complexes are the mechanotransducers between the extracellular matrix and the actomyosin cytoskeleton. By analogy, cadherin complexes may function as mechanosensors at cell–cell junctions, but this capacity of cadherins has not been directly demonstrated. Furthermore, the molecular composition of the link between E-cadherin and actin, which is needed to sustain such a function, is unresolved. In this study, we describe nanomechanical measurements demonstrating that E-cadherin complexes are functional mechanosensors that transmit force between F-actin and E-cadherin. Imaging experiments reveal that intercellular forces coincide with vinculin accumulation at actin-anchored cadherin adhesions, and nanomechanical measurements show that vinculin potentiates the E-cadherin mechanosensory response. These investigations directly demonstrate the mechanosensory capacity of the E-cadherin complex and identify a novel function for vinculin at cell–cell junctions. These findings have implications for barrier function, morphogenesis, cell migration, and invasion and may extend to all soft tissues in which classical cadherins regulate cell–cell adhesion.

Bibliography

le Duc, Q., Shi, Q., Blonk, I., Sonnenberg, A., Wang, N., Leckband, D., & de Rooij, J. (2010). Vinculin potentiates E-cadherin mechanosensing and is recruited to actin-anchored sites within adherens junctions in a myosin IIâdependent manner. Journal of Cell Biology, 189(7), 1107â1115.

Authors 7

Quint le Duc (first)
Quanming Shi (additional)
Iris Blonk (additional)
Arnoud Sonnenberg (additional)
Ning Wang (additional)
Deborah Leckband (additional)
Johan de Rooij (additional)

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Dates

Type	When
Created	15 years, 1 month ago (June 28, 2010, 10:33 a.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 9:18 p.m.)
Indexed	8 hours, 47 minutes ago (Aug. 26, 2025, 3:10 a.m.)
Issued	15 years, 1 month ago (June 28, 2010)
Published	15 years, 1 month ago (June 28, 2010)
Published Online	15 years, 1 month ago (June 28, 2010)
Published Print	15 years, 1 month ago (June 28, 2010)

Funders 0

None

BibTeX

@article{le_Duc_2010, title={Vinculin potentiates E-cadherin mechanosensing and is recruited to actin-anchored sites within adherens junctions in a myosin II–dependent manner}, volume={189}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.201001149}, DOI={10.1083/jcb.201001149}, number={7}, journal={Journal of Cell Biology}, publisher={Rockefeller University Press}, author={le Duc, Quint and Shi, Quanming and Blonk, Iris and Sonnenberg, Arnoud and Wang, Ning and Leckband, Deborah and de Rooij, Johan}, year={2010}, month=jun, pages={1107–1115} }

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