DOI: 10.1083/jcb.200810030. Role of the Ndc1 interaction network in yeast nuclear pore complex assembly and maintenance

Role of the Ndc1 interaction network in yeast nuclear pore complex assembly and maintenance

10.1083/jcb.200810030

Crossref journal-article

Rockefeller University Press

Journal of Cell Biology (291)

Abstract

The nuclear pore complex (NPC) mediates all nucleocytoplasmic transport, yet its structure and biogenesis remain poorly understood. In this study, we have functionally characterized interaction partners of the yeast transmembrane nucleoporin Ndc1. Ndc1 forms a distinct complex with the transmembrane proteins Pom152 and Pom34 and two alternative complexes with the soluble nucleoporins Nup53 and Nup59, which in turn bind to Nup170 and Nup157. The transmembrane and soluble Ndc1-binding partners have redundant functions at the NPC, and disruption of both groups of interactions causes defects in Ndc1 targeting and in NPC structure accompanied by significant pore dilation. Using photoconvertible fluorescent protein fusions, we further show that the depletion of Pom34 in cells that lack NUP53 and NUP59 blocks new NPC assembly and leads to the reversible accumulation of newly made nucleoporins in cytoplasmic foci. Therefore, Ndc1 together with its interaction partners are collectively essential for the biosynthesis and structural integrity of yeast NPCs.

Bibliography

Onischenko, E., Stanton, L. H., Madrid, A. S., Kieselbach, T., & Weis, K. (2009). Role of the Ndc1 interaction network in yeast nuclear pore complex assembly and maintenance. Journal of Cell Biology, 185(3), 475â491.

Authors 5

Evgeny Onischenko (first)
Leslie H. Stanton (additional)
Alexis S. Madrid (additional)
Thomas Kieselbach (additional)
Karsten Weis (additional)

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Dates

Type	When
Created	16 years, 3 months ago (May 4, 2009, 2:12 p.m.)
Deposited	2 years ago (July 29, 2023, 8:15 a.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 8:26 p.m.)
Issued	16 years, 3 months ago (May 4, 2009)
Published	16 years, 3 months ago (May 4, 2009)
Published Online	16 years, 3 months ago (May 4, 2009)
Published Print	16 years, 3 months ago (May 4, 2009)

Funders 0

None

BibTeX

@article{Onischenko_2009, title={Role of the Ndc1 interaction network in yeast nuclear pore complex assembly and maintenance}, volume={185}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200810030}, DOI={10.1083/jcb.200810030}, number={3}, journal={Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Onischenko, Evgeny and Stanton, Leslie H. and Madrid, Alexis S. and Kieselbach, Thomas and Weis, Karsten}, year={2009}, month=may, pages={475–491} }

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