DOI: 10.1083/jcb.200806112. AnkyrinG is required for maintenance of the axon initial segment and neuronal polarity

AnkyrinG is required for maintenance of the axon initial segment and neuronal polarity

10.1083/jcb.200806112

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The axon initial segment (AIS) functions as both a physiological and physical bridge between somatodendritic and axonal domains. Given its unique molecular composition, location, and physiology, the AIS is thought to maintain neuronal polarity. To identify the molecular basis of this AIS property, we used adenovirus-mediated RNA interference to silence AIS protein expression in polarized neurons. Some AIS proteins are remarkably stable with half-lives of at least 2 wk. However, silencing the expression of the cytoskeletal scaffold ankyrinG (ankG) dismantles the AIS and causes axons to acquire the molecular characteristics of dendrites. Both cytoplasmic- and membrane-associated proteins, which are normally restricted to somatodendritic domains, redistribute into the former axon. Furthermore, spines and postsynaptic densities of excitatory synapses assemble on former axons. Our results demonstrate that the loss of ankG causes axons to acquire the molecular characteristics of dendrites; thus, ankG is required for the maintenance of neuronal polarity and molecular organization of the AIS.

Bibliography

Hedstrom, K. L., Ogawa, Y., & Rasband, M. N. (2008). AnkyrinG is required for maintenance of the axon initial segment and neuronal polarity. The Journal of Cell Biology, 183(4), 635â640.

Authors 3

Kristian L. Hedstrom (first)
Yasuhiro Ogawa (additional)
Matthew N. Rasband (additional)

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Dates

Type	When
Created	16 years, 9 months ago (Nov. 17, 2008, 12:19 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 4:35 p.m.)
Indexed	1 month, 1 week ago (July 20, 2025, 12:25 a.m.)
Issued	16 years, 9 months ago (Nov. 10, 2008)
Published	16 years, 9 months ago (Nov. 10, 2008)
Published Online	16 years, 9 months ago (Nov. 10, 2008)
Published Print	16 years, 9 months ago (Nov. 17, 2008)

Funders 0

None

BibTeX

@article{Hedstrom_2008, title={AnkyrinG is required for maintenance of the axon initial segment and neuronal polarity}, volume={183}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200806112}, DOI={10.1083/jcb.200806112}, number={4}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Hedstrom, Kristian L. and Ogawa, Yasuhiro and Rasband, Matthew N.}, year={2008}, month=nov, pages={635–640} }

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