DOI: 10.1083/jcb.200804170. Orientation and structure of the Ndc80 complex on the microtubule lattice

Orientation and structure of the Ndc80 complex on the microtubule lattice

10.1083/jcb.200804170

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The four-subunit Ndc80 complex, comprised of Ndc80/Nuf2 and Spc24/Spc25 dimers, directly connects kinetochores to spindle microtubules. The complex is anchored to the kinetochore at the Spc24/25 end, and the Ndc80/Nuf2 dimer projects outward to bind to microtubules. Here, we use cryoelectron microscopy and helical image analysis to visualize the interaction of the Ndc80/Nuf2 dimer with microtubules. Our results, when combined with crystallography data, suggest that the globular domain of the Ndc80 subunit binds strongly at the interface between tubulin dimers and weakly at the adjacent intradimer interface along the protofilament axis. Such a binding mode, in which the Ndc80 complex interacts with sequential α/β-tubulin heterodimers, may be important for stabilizing kinetochore-bound microtubules. Additionally, we define the binding of the Ndc80 complex relative to microtubule polarity, which reveals that the microtubule interaction surface is at a considerable distance from the opposite kinetochore-anchored end; this binding geometry may facilitate polymerization and depolymerization at kinetochore-attached microtubule ends.

Bibliography

Wilson-Kubalek, E. M., Cheeseman, I. M., Yoshioka, C., Desai, A., & Milligan, R. A. (2008). Orientation and structure of the Ndc80 complex on the microtubule lattice. The Journal of Cell Biology, 182(6), 1055â1061.

Authors 5

Elizabeth M. Wilson-Kubalek (first)
Iain M. Cheeseman (additional)
Craig Yoshioka (additional)
Arshad Desai (additional)
Ronald A. Milligan (additional)

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Dates

Type	When
Created	16 years, 11 months ago (Sept. 15, 2008, 9:09 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 7:40 p.m.)
Indexed	2 months, 1 week ago (June 16, 2025, 2:43 a.m.)
Issued	16 years, 11 months ago (Sept. 15, 2008)
Published	16 years, 11 months ago (Sept. 15, 2008)
Published Online	16 years, 11 months ago (Sept. 15, 2008)
Published Print	16 years, 11 months ago (Sept. 22, 2008)

Funders 0

None

BibTeX

@article{Wilson_Kubalek_2008, title={Orientation and structure of the Ndc80 complex on the microtubule lattice}, volume={182}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200804170}, DOI={10.1083/jcb.200804170}, number={6}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Wilson-Kubalek, Elizabeth M. and Cheeseman, Iain M. and Yoshioka, Craig and Desai, Arshad and Milligan, Ronald A.}, year={2008}, month=sep, pages={1055–1061} }

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