DOI: 10.1083/jcb.200803174. Association between active genes occurs at nuclear speckles and is modulated by chromatin environment

Association between active genes occurs at nuclear speckles and is modulated by chromatin environment

10.1083/jcb.200803174

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Genes on different chromosomes can be spatially associated in the nucleus in several transcriptional and regulatory situations; however, the functional significance of such associations remains unclear. Using human erythropoiesis as a model, we show that five cotranscribed genes, which are found on four different chromosomes, associate with each other at significant but variable frequencies. Those genes most frequently in association lie in decondensed stretches of chromatin. By replacing the mouse α-globin gene cluster in situ with its human counterpart, we demonstrate a direct effect of the regional chromatin environment on the frequency of association, whereas nascent transcription from the human α-globin gene appears unaffected. We see no evidence that cotranscribed erythroid genes associate at shared transcription foci, but we do see stochastic clustering of active genes around common nuclear SC35-enriched speckles (hence the apparent nonrandom association between genes). Thus, association between active genes may result from their location on decondensed chromatin that enables clustering around common nuclear speckles.

Bibliography

Brown, J. M., Green, J., das Neves, R. P., Wallace, H. A. C., Smith, A. J. H., Hughes, J., Gray, N., Taylor, S., Wood, W. G., Higgs, D. R., Iborra, F. J., & Buckle, V. J. (2008). Association between active genes occurs at nuclear speckles and is modulated by chromatin environment. The Journal of Cell Biology, 182(6), 1083â1097.

Authors 12

Jill M. Brown (first)
Joanne Green (additional)
Ricardo Pires das Neves (additional)
Helen A.C. Wallace (additional)
Andrew J.H. Smith (additional)
Jim Hughes (additional)
Nicki Gray (additional)
Steve Taylor (additional)
William G. Wood (additional)
Douglas R. Higgs (additional)
Francisco J. Iborra (additional)
Veronica J. Buckle (additional)

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Dates

Type	When
Created	16 years, 11 months ago (Sept. 22, 2008, 1:53 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 7:51 p.m.)
Indexed	10 minutes ago (Aug. 29, 2025, 9:16 a.m.)
Issued	16 years, 11 months ago (Sept. 22, 2008)
Published	16 years, 11 months ago (Sept. 22, 2008)
Published Online	16 years, 11 months ago (Sept. 22, 2008)
Published Print	16 years, 11 months ago (Sept. 22, 2008)

Funders 0

None

BibTeX

@article{Brown_2008, title={Association between active genes occurs at nuclear speckles and is modulated by chromatin environment}, volume={182}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200803174}, DOI={10.1083/jcb.200803174}, number={6}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Brown, Jill M. and Green, Joanne and das Neves, Ricardo Pires and Wallace, Helen A.C. and Smith, Andrew J.H. and Hughes, Jim and Gray, Nicki and Taylor, Steve and Wood, William G. and Higgs, Douglas R. and Iborra, Francisco J. and Buckle, Veronica J.}, year={2008}, month=sep, pages={1083–1097} }

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