DOI: 10.1083/jcb.200711154. Reversible cytoplasmic localization of the proteasome in quiescent yeast cells

Reversible cytoplasmic localization of the proteasome in quiescent yeast cells

10.1083/jcb.200711154

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The 26S proteasome is responsible for the controlled proteolysis of a vast number of proteins, including crucial cell cycle regulators. Accordingly, in Saccharomyces cerevisiae, 26S proteasome function is mandatory for cell cycle progression. In budding yeast, the 26S proteasome is assembled in the nucleus, where it is localized throughout the cell cycle. We report that upon cell entry into quiescence, proteasome subunits massively relocalize from the nucleus into motile cytoplasmic structures. We further demonstrate that these structures are proteasome cytoplasmic reservoirs that are rapidly mobilized upon exit from quiescence. Therefore, we have named these previously unknown structures proteasome storage granules (PSGs). Finally, we observe conserved formation and mobilization of these PSGs in the evolutionary distant yeast Schizosaccharomyces pombe. This conservation implies a broad significance for these proteasome reserves.

Bibliography

Laporte, D., Salin, B., Daignan-Fornier, B., & Sagot, I. (2008). Reversible cytoplasmic localization of the proteasome in quiescent yeast cells. The Journal of Cell Biology, 181(5), 737â745.

Authors 4

Damien Laporte (first)
Bénédicte Salin (additional)
Bertrand Daignan-Fornier (additional)
Isabelle Sagot (additional)

References 26 Referenced 182

10.1016/S0960-9822(03)00417-2 / Curr. Biol. (2003)
10.1073/pnas.082243699 / Proc. Natl. Acad. Sci. USA. (2002)
10.1016/j.gde.2006.02.009 / Curr. Opin. Genet. Dev. (2006)
10.1093/emboj/17.21.6144 / EMBO J. (1998)
10.1038/sj.embor.embor938 / EMBO Rep. (2003)
10.1016/S1097-2765(01)00250-7 / Mol. Cell. (2001)
10.1016/j.febslet.2007.03.053 / FEBS Lett. (2007)
{'key': '2023072202343843500_BIB8', 'first-page': '1562', 'volume': '61', 'year': '2004', 'journal-title': 'Cell. Mol. Life Sci.'} / Cell. Mol. Life Sci. (2004)
10.1091/mbc.e06-07-0635 / Mol. Biol. Cell. (2007)
10.1016/S0962-8924(00)01852-3 / Trends Cell Biol. (2000)
10.1074/jbc.271.44.27280 / J. Biol. Chem. (1996)
10.1006/jmbi.2002.5443 / J. Mol. Biol. (2002)
10.1038/sj.emboj.7601681 / EMBO J. (2007)
10.1083/jcb.137.3.539 / J. Cell Biol. (1997)
10.1016/S0003-9861(03)00197-8 / Arch. Biochem. Biophys. (2003)
10.1016/j.molcel.2007.02.011 / Mol. Cell. (2007)
10.1074/jbc.274.31.21943 / J. Biol. Chem. (1999)
10.1038/ncb719 / Nat. Cell Biol. (2002)
10.1091/mbc.e06-04-0282 / Mol. Biol. Cell. (2006)
{'key': '2023072202343843500_BIB20', 'first-page': '725', 'volume': '386', 'year': '2005', 'journal-title': 'Biol. Chem.'} / Biol. Chem. (2005)
10.1093/nar/gkl012 / Nucleic Acids Res. (2006)
10.1016/j.cell.2005.05.023 / Cell. (2005)
10.1016/S0960-9822(00)00773-9 / Curr. Biol. (2000)
10.1074/jbc.M403551200 / J. Biol. Chem. (2004)
10.1093/emboj/17.22.6465 / EMBO J. (1998)
10.1016/S1357-2725(02)00380-1 / Int. J. Biochem. Cell Biol. (2003)

Dates

Type	When
Created	17 years, 2 months ago (May 26, 2008, 8:34 p.m.)
Deposited	2 years, 1 month ago (July 21, 2023, 10:35 p.m.)
Indexed	4 weeks, 1 day ago (July 24, 2025, 7:25 a.m.)
Issued	17 years, 2 months ago (May 26, 2008)
Published	17 years, 2 months ago (May 26, 2008)
Published Online	17 years, 2 months ago (May 26, 2008)
Published Print	17 years, 2 months ago (June 2, 2008)

Funders 0

None

BibTeX

@article{Laporte_2008, title={Reversible cytoplasmic localization of the proteasome in quiescent yeast cells}, volume={181}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200711154}, DOI={10.1083/jcb.200711154}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Laporte, Damien and Salin, Bénédicte and Daignan-Fornier, Bertrand and Sagot, Isabelle}, year={2008}, month=may, pages={737–745} }

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