DOI: 10.1083/jcb.200710058. Actin-dependent intranuclear repositioning of an active gene locus in vivo

Actin-dependent intranuclear repositioning of an active gene locus in vivo

10.1083/jcb.200710058

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Although bulk chromatin is thought to have limited mobility within the interphase eukaryotic nucleus, directed long-distance chromosome movements are not unknown. Cajal bodies (CBs) are nuclear suborganelles that nonrandomly associate with small nuclear RNA (snRNA) and histone gene loci in human cells during interphase. However, the mechanism responsible for this association is uncertain. In this study, we present an experimental system to probe the dynamic interplay of CBs with a U2 snRNA target gene locus during transcriptional activation in living cells. Simultaneous four-dimensional tracking of CBs and U2 genes reveals that target loci are recruited toward relatively stably positioned CBs by long-range chromosomal motion. In the presence of a dominant-negative mutant of β-actin, the repositioning of activated U2 genes is markedly inhibited. This supports a model in which nuclear actin is required for these rapid, long-range chromosomal movements.

Bibliography

Dundr, M., Ospina, J. K., Sung, M.-H., John, S., Upender, M., Ried, T., Hager, G. L., & Matera, A. G. (2007). Actin-dependent intranuclear repositioning of an active gene locus in vivo. The Journal of Cell Biology, 179(6), 1095â1103.

Authors 8

Miroslav Dundr (first)
Jason K. Ospina (additional)
Myong-Hee Sung (additional)
Sam John (additional)
Madhvi Upender (additional)
Thomas Ried (additional)
Gordon L. Hager (additional)
A. Gregory Matera (additional)

References 23 Referenced 257

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Dates

Type	When
Created	17 years, 8 months ago (Dec. 10, 2007, 8:34 p.m.)
Deposited	2 years ago (July 29, 2023, 11:18 a.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:33 p.m.)
Issued	17 years, 8 months ago (Dec. 10, 2007)
Published	17 years, 8 months ago (Dec. 10, 2007)
Published Online	17 years, 8 months ago (Dec. 10, 2007)
Published Print	17 years, 8 months ago (Dec. 17, 2007)

Funders 0

None

BibTeX

@article{Dundr_2007, title={Actin-dependent intranuclear repositioning of an active gene locus in vivo}, volume={179}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200710058}, DOI={10.1083/jcb.200710058}, number={6}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Dundr, Miroslav and Ospina, Jason K. and Sung, Myong-Hee and John, Sam and Upender, Madhvi and Ried, Thomas and Hager, Gordon L. and Matera, A. Gregory}, year={2007}, month=dec, pages={1095–1103} }

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