DOI: 10.1083/jcb.200706018. The transcriptional cycle of HIV-1 in real-time and live cells

The transcriptional cycle of HIV-1 in real-time and live cells

10.1083/jcb.200706018

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

RNA polymerase II (RNAPII) is a fundamental enzyme, but few studies have analyzed its activity in living cells. Using human immunodeficiency virus (HIV) type 1 reporters, we study real-time messenger RNA (mRNA) biogenesis by photobleaching nascent RNAs and RNAPII at specific transcription sites. Through modeling, the use of mutant polymerases, drugs, and quantitative in situ hybridization, we investigate the kinetics of the HIV-1 transcription cycle. Initiation appears efficient because most polymerases demonstrate stable gene association. We calculate an elongation rate of approximately 1.9 kb/min, and, surprisingly, polymerases remain at transcription sites 2.5 min longer than nascent RNAs. With a total polymerase residency time estimated at 333 s, 114 are assigned to elongation, and 63 are assigned to 3′-end processing and/or transcript release. However, mRNAs were released seconds after polyadenylation onset, and analysis of polymerase density by chromatin immunoprecipitation suggests that they pause or lose processivity after passing the polyA site. The strengths and limitations of this kinetic approach to analyze mRNA biogenesis in living cells are discussed.

Bibliography

Boireau, S., Maiuri, P., Basyuk, E., de la Mata, M., Knezevich, A., Pradet-Balade, B., BaÌcker, V., Kornblihtt, A., Marcello, A., & Bertrand, E. (2007). The transcriptional cycle of HIV-1 in real-time and live cells. The Journal of Cell Biology, 179(2), 291â304.

Authors 10

Stéphanie Boireau (first)
Paolo Maiuri (additional)
Eugenia Basyuk (additional)
Manuel de la Mata (additional)
Anna Knezevich (additional)
Bérangère Pradet-Balade (additional)
Volker Bäcker (additional)
Alberto Kornblihtt (additional)
Alessandro Marcello (additional)
Edouard Bertrand (additional)

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Dates

Type	When
Created	17 years, 10 months ago (Oct. 22, 2007, 2:44 p.m.)
Deposited	2 years ago (July 29, 2023, 7:46 a.m.)
Indexed	2 weeks, 3 days ago (Aug. 6, 2025, 8:11 a.m.)
Issued	17 years, 10 months ago (Oct. 22, 2007)
Published	17 years, 10 months ago (Oct. 22, 2007)
Published Online	17 years, 10 months ago (Oct. 22, 2007)
Published Print	17 years, 10 months ago (Oct. 22, 2007)

Funders 0

None

BibTeX

@article{Boireau_2007, title={The transcriptional cycle of HIV-1 in real-time and live cells}, volume={179}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200706018}, DOI={10.1083/jcb.200706018}, number={2}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Boireau, Stéphanie and Maiuri, Paolo and Basyuk, Eugenia and de la Mata, Manuel and Knezevich, Anna and Pradet-Balade, Bérangère and Bäcker, Volker and Kornblihtt, Alberto and Marcello, Alessandro and Bertrand, Edouard}, year={2007}, month=oct, pages={291–304} }

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