DOI: 10.1083/jcb.200705119. Neurofascin assembles a specialized extracellular matrix at the axon initial segment

Neurofascin assembles a specialized extracellular matrix at the axon initial segment

10.1083/jcb.200705119

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Action potential initiation and propagation requires clustered Na+ (voltage-gated Na+ [Nav]) channels at axon initial segments (AIS) and nodes of Ranvier. In addition to ion channels, these domains are characterized by cell adhesion molecules (CAMs; neurofascin-186 [NF-186] and neuron glia–related CAM [NrCAM]), cytoskeletal proteins (ankyrinG and βIV spectrin), and the extracellular chondroitin-sulfate proteoglycan brevican. Schwann cells initiate peripheral nervous system node formation by clustering NF-186, which then recruits ankyrinG and Nav channels. However, AIS assembly of this protein complex does not require glial contact. To determine the AIS assembly mechanism, we silenced expression of AIS proteins by RNA interference. AnkyrinG knockdown prevented AIS localization of all other AIS proteins. Loss of NF-186, NrCAM, Nav channels, or βIV spectrin did not affect other neuronal AIS proteins. However, loss of NF-186 blocked assembly of the brevican-based AIS extracellular matrix, and NF-186 overexpression caused somatodendritic brevican clustering. Thus, NF-186 assembles and links the specialized brevican-containing AIS extracellular matrix to the intracellular cytoskeleton.

Bibliography

Hedstrom, K. L., Xu, X., Ogawa, Y., Frischknecht, R., Seidenbecher, C. I., Shrager, P., & Rasband, M. N. (2007). Neurofascin assembles a specialized extracellular matrix at the axon initial segment. The Journal of Cell Biology, 178(5), 875â886.

Authors 7

Kristian L. Hedstrom (first)
Xiaorong Xu (additional)
Yasuhiro Ogawa (additional)
Renato Frischknecht (additional)
Constanze I. Seidenbecher (additional)
Peter Shrager (additional)
Matthew N. Rasband (additional)

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Dates

Type	When
Created	18 years ago (Aug. 20, 2007, 8:59 p.m.)
Deposited	2 years, 1 month ago (July 29, 2023, 7:45 a.m.)
Indexed	4 weeks, 1 day ago (Aug. 5, 2025, 8:36 a.m.)
Issued	18 years ago (Aug. 20, 2007)
Published	18 years ago (Aug. 20, 2007)
Published Online	18 years ago (Aug. 20, 2007)
Published Print	18 years ago (Aug. 27, 2007)

Funders 0

None

BibTeX

@article{Hedstrom_2007, title={Neurofascin assembles a specialized extracellular matrix at the axon initial segment}, volume={178}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200705119}, DOI={10.1083/jcb.200705119}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Hedstrom, Kristian L. and Xu, Xiaorong and Ogawa, Yasuhiro and Frischknecht, Renato and Seidenbecher, Constanze I. and Shrager, Peter and Rasband, Matthew N.}, year={2007}, month=aug, pages={875–886} }

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