DOI: 10.1083/jcb.200705002. Ena/VASP is required for endothelial barrier function in vivo

Ena/VASP is required for endothelial barrier function in vivo

10.1083/jcb.200705002

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Enabled/vasodilator-stimulated phosphoprotein (Ena/VASP) proteins are key actin regulators that localize at regions of dynamic actin remodeling, including cellular protrusions and cell–cell and cell–matrix junctions. Several studies have suggested that Ena/VASP proteins are involved in the formation and function of cellular junctions. Here, we establish the importance of Ena/VASP in endothelial junctions in vivo by analysis of Ena/VASP-deficient animals. In the absence of Ena/VASP, the vasculature exhibits patterning defects and lacks structural integrity, leading to edema, hemorrhaging, and late stage embryonic lethality. In endothelial cells, we find that Ena/VASP activity is required for normal F-actin content, actomyosin contractility, and proper response to shear stress. These findings demonstrate that Ena/VASP is critical for actin cytoskeleton remodeling events involved in the maintenance of functional endothelia.

Authors 8

Craig Furman (first)
Alisha L. Sieminski (additional)
Adam V. Kwiatkowski (additional)
Douglas A. Rubinson (additional)
Eliza Vasile (additional)
Roderick T. Bronson (additional)
Reinhard Fässler (additional)
Frank B. Gertler (additional)

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Dates

Type	When
Created	17 years, 9 months ago (Nov. 12, 2007, 9:07 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 3:59 p.m.)
Indexed	1 year, 1 month ago (July 21, 2024, 12:32 p.m.)
Issued	17 years, 9 months ago (Nov. 12, 2007)
Published	17 years, 9 months ago (Nov. 12, 2007)
Published Online	17 years, 9 months ago (Nov. 12, 2007)
Published Print	17 years, 9 months ago (Nov. 19, 2007)

Funders 0

None

BibTeX

@article{Furman_2007, title={Ena/VASP is required for endothelial barrier function in vivo}, volume={179}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200705002}, DOI={10.1083/jcb.200705002}, number={4}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Furman, Craig and Sieminski, Alisha L. and Kwiatkowski, Adam V. and Rubinson, Douglas A. and Vasile, Eliza and Bronson, Roderick T. and Fässler, Reinhard and Gertler, Frank B.}, year={2007}, month=nov, pages={761–775} }

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