DOI: 10.1083/jcb.200703178. The motor activity of myosin-X promotes actin fiber convergence at the cell periphery to initiate filopodia formation

The motor activity of myosin-X promotes actin fiber convergence at the cell periphery to initiate filopodia formation

10.1083/jcb.200703178

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Filopodia are actin-rich fingerlike protrusions found at the leading edge of migrating cells and are believed to play a role in directional sensing. Previous studies have shown that myosin-X (myoX) promotes filopodia formation and that this is mediated through its ability to deliver specific cargoes to the cell periphery (Tokuo, H., and M. Ikebe. 2004. Biochem Biophys. Commun. 319:214–220; Zhang, H., J.S. Berg, Z. Li, Y. Wang, P. Lang, A.D. Sousa, A. Bhaskar, R.E. Cheney, and S. Stromblad. 2004. Nat. Cell Biol. 6:523–531; Bohil, A.B., B.W. Robertson, and R.E. Cheney. 2006. Proc. Natl. Acad. Sci. USA. 103:12411–12416; Zhu, X.J., C.Z. Wang, P.G. Dai, Y. Xie, N.N. Song, Y. Liu, Q.S. Du, L. Mei, Y.Q. Ding, and W.C. Xiong. 2007. Nat. Cell Biol. 9:184–192). In this study, we show that the motor function of myoX and not the cargo function is critical for initiating filopodia formation. Using a dimer-inducing technique, we find that myoX lacking its cargo-binding tail moves laterally at the leading edge of lamellipodia and induces filopodia in living cells. We conclude that the motor function of the two-headed form of myoX is critical for actin reorganization at the leading edge, leading to filopodia formation.

Bibliography

Tokuo, H., Mabuchi, K., & Ikebe, M. (2007). The motor activity of myosin-X promotes actin fiber convergence at the cell periphery to initiate filopodia formation. The Journal of Cell Biology, 179(2), 229â238.

Authors 3

Hiroshi Tokuo (first)
Katsuhide Mabuchi (additional)
Mitsuo Ikebe (additional)

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Dates

Type	When
Created	17 years, 10 months ago (Oct. 22, 2007, 2:44 p.m.)
Deposited	2 years, 1 month ago (July 29, 2023, 5:19 a.m.)
Indexed	1 month, 1 week ago (July 28, 2025, 5:33 p.m.)
Issued	17 years, 10 months ago (Oct. 22, 2007)
Published	17 years, 10 months ago (Oct. 22, 2007)
Published Online	17 years, 10 months ago (Oct. 22, 2007)
Published Print	17 years, 10 months ago (Oct. 22, 2007)

Funders 0

None

BibTeX

@article{Tokuo_2007, title={The motor activity of myosin-X promotes actin fiber convergence at the cell periphery to initiate filopodia formation}, volume={179}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200703178}, DOI={10.1083/jcb.200703178}, number={2}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Tokuo, Hiroshi and Mabuchi, Katsuhide and Ikebe, Mitsuo}, year={2007}, month=oct, pages={229–238} }

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