DOI: 10.1083/jcb.200612135. Distinct functions of the <i>Drosophila</i> Nup153 and Nup214 FG domains in nuclear protein transport

Distinct functions of the Drosophila Nup153 and Nup214 FG domains in nuclear protein transport

10.1083/jcb.200612135

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The phenylanine-glycine (FG)–rich regions of several nucleoporins both bind to nuclear transport receptors and collectively provide a diffusion barrier to the nuclear pores. However, the in vivo roles of FG nucleoporins in transport remain unclear. We have inactivated 30 putative nucleoporins in cultured Drosophila melanogaster S2 cells by RNA interference and analyzed the phenotypes on importin α/β−mediated import and CRM1-dependent protein export. The fly homologues of FG nucleoporins Nup358, Nup153, and Nup54 are selectively required for import. The FG repeats of Nup153 are necessary for its function in transport, whereas the remainder of the protein maintains pore integrity. Inactivation of the CRM1 cofactor RanBP3 decreased the nuclear accumulation of CRM1 and protein export. We report a surprisingly antagonistic relationship between RanBP3 and the Nup214 FG region in determining CRM1 localization and its function in protein export. Our data suggest that peripheral metazoan FG nucleoporins have distinct functions in nuclear protein transport events.

Bibliography

Sabri, N., Roth, P., Xylourgidis, N., Sadeghifar, F., Adler, J., & Samakovlis, C. (2007). Distinct functions of the Drosophila Nup153 and Nup214 FG domains in nuclear protein transport. The Journal of Cell Biology, 178(4), 557â565.

Authors 6

Nafiseh Sabri (first)
Peggy Roth (additional)
Nikos Xylourgidis (additional)
Fatemeh Sadeghifar (additional)
Jeremy Adler (additional)
Christos Samakovlis (additional)

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Dates

Type	When
Created	18 years ago (Aug. 6, 2007, 8:54 p.m.)
Deposited	2 years ago (July 29, 2023, 5:22 a.m.)
Indexed	3 months, 1 week ago (May 14, 2025, 11:25 a.m.)
Issued	18 years ago (Aug. 6, 2007)
Published	18 years ago (Aug. 6, 2007)
Published Online	18 years ago (Aug. 6, 2007)
Published Print	18 years ago (Aug. 13, 2007)

Funders 0

None

BibTeX

@article{Sabri_2007, title={Distinct functions of the Drosophila Nup153 and Nup214 FG domains in nuclear protein transport}, volume={178}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200612135}, DOI={10.1083/jcb.200612135}, number={4}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Sabri, Nafiseh and Roth, Peggy and Xylourgidis, Nikos and Sadeghifar, Fatemeh and Adler, Jeremy and Samakovlis, Christos}, year={2007}, month=aug, pages={557–565} }

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