DOI: 10.1083/jcb.200610144. Regulation of connexin43 gap junctional communication by phosphatidylinositol 4,5-bisphosphate

Regulation of connexin43 gap junctional communication by phosphatidylinositol 4,5-bisphosphate

10.1083/jcb.200610144

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Cell–cell communication through connexin43 (Cx43)-based gap junction channels is rapidly inhibited upon activation of various G protein–coupled receptors; however, the mechanism is unknown. We show that Cx43-based cell–cell communication is inhibited by depletion of phosphatidylinositol 4,5-bisphosphate (PtdIns[4,5]P2) from the plasma membrane. Knockdown of phospholipase Cβ3 (PLCβ3) inhibits PtdIns(4,5)P2 hydrolysis and keeps Cx43 channels open after receptor activation. Using a translocatable 5-phosphatase, we show that PtdIns(4,5)P2 depletion is sufficient to close Cx43 channels. When PtdIns(4,5)P2 is overproduced by PtdIns(4)P 5-kinase, Cx43 channel closure is impaired. We find that the Cx43 binding partner zona occludens 1 (ZO-1) interacts with PLCβ3 via its third PDZ domain. ZO-1 is essential for PtdIns(4,5)P2-hydrolyzing receptors to inhibit cell–cell communication, but not for receptor–PLC coupling. Our results show that PtdIns(4,5)P2 is a key regulator of Cx43 channel function, with no role for other second messengers, and suggest that ZO-1 assembles PLCβ3 and Cx43 into a signaling complex to allow regulation of cell–cell communication by localized changes in PtdIns(4,5)P2.

Bibliography

van Zeijl, L., Ponsioen, B., Giepmans, B. N. G., Ariaens, A., Postma, F. R., VaÌrnai, P., Balla, T., Divecha, N., Jalink, K., & Moolenaar, W. H. (2007). Regulation of connexin43 gap junctional communication by phosphatidylinositol 4,5-bisphosphate. The Journal of Cell Biology, 177(5), 881â891.

Authors 10

Leonie van Zeijl (first)
Bas Ponsioen (additional)
Ben N.G. Giepmans (additional)
Aafke Ariaens (additional)
Friso R. Postma (additional)
Péter Várnai (additional)
Tamas Balla (additional)
Nullin Divecha (additional)
Kees Jalink (additional)
Wouter H. Moolenaar (additional)

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Dates

Type	When
Created	18 years, 2 months ago (May 29, 2007, 9:34 p.m.)
Deposited	2 years ago (July 29, 2023, 4:58 a.m.)
Indexed	4 months ago (April 22, 2025, 4:10 a.m.)
Issued	18 years, 2 months ago (May 29, 2007)
Published	18 years, 2 months ago (May 29, 2007)
Published Online	18 years, 2 months ago (May 29, 2007)
Published Print	18 years, 2 months ago (June 4, 2007)

Funders 0

None

BibTeX

@article{van_Zeijl_2007, title={Regulation of connexin43 gap junctional communication by phosphatidylinositol 4,5-bisphosphate}, volume={177}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200610144}, DOI={10.1083/jcb.200610144}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={van Zeijl, Leonie and Ponsioen, Bas and Giepmans, Ben N.G. and Ariaens, Aafke and Postma, Friso R. and Várnai, Péter and Balla, Tamas and Divecha, Nullin and Jalink, Kees and Moolenaar, Wouter H.}, year={2007}, month=may, pages={881–891} }

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