DOI: 10.1083/jcb.200608071. ZBP1 regulates mRNA stability during cellular stress

ZBP1 regulates mRNA stability during cellular stress

10.1083/jcb.200608071

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

An essential constituent of the integrated stress response (ISR) is a reversible translational suppression. This mRNA silencing occurs in distinct cytoplasmic foci called stress granules (SGs), which transiently associate with processing bodies (PBs), typically serving as mRNA decay centers. How mRNAs are protected from degradation in these structures remains elusive. We identify that Zipcode-binding protein 1 (ZBP1) regulates the cytoplasmic fate of specific mRNAs in nonstressed cells and is a key regulator of mRNA turnover during the ISR. ZBP1 association with target mRNAs in SGs was not essential for mRNA targeting to SGs. However, ZBP1 knockdown induced a selective destabilization of target mRNAs during the ISR, whereas forced expression increased mRNA stability. Our results indicate that although targeting of mRNAs to SGs is nonspecific, the stabilization of mRNAs during cellular stress requires specific protein–mRNA interactions. These retain mRNAs in SGs and prevent premature decay in PBs. Hence, mRNA-binding proteins are essential for translational adaptation during cellular stress by modulating mRNA turnover.

Bibliography

StoÌhr, N., Lederer, M., Reinke, C., Meyer, S., Hatzfeld, M., Singer, R. H., & HuÌttelmaier, S. (2006). ZBP1 regulates mRNA stability during cellular stress. The Journal of Cell Biology, 175(4), 527â534.

Authors 7

Nadine Stöhr (first)
Marcell Lederer (additional)
Claudia Reinke (additional)
Sylke Meyer (additional)
Mechthild Hatzfeld (additional)
Robert H. Singer (additional)
Stefan Hüttelmaier (additional)

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Dates

Type	When
Created	18 years, 9 months ago (Nov. 13, 2006, 9:34 p.m.)
Deposited	2 years, 1 month ago (July 29, 2023, 6:21 a.m.)
Indexed	1 month, 1 week ago (July 26, 2025, 5:16 a.m.)
Issued	18 years, 9 months ago (Nov. 13, 2006)
Published	18 years, 9 months ago (Nov. 13, 2006)
Published Online	18 years, 9 months ago (Nov. 13, 2006)
Published Print	18 years, 9 months ago (Nov. 20, 2006)

Funders 0

None

BibTeX

@article{Sto_hr_2006, title={ZBP1 regulates mRNA stability during cellular stress}, volume={175}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200608071}, DOI={10.1083/jcb.200608071}, number={4}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Stöhr, Nadine and Lederer, Marcell and Reinke, Claudia and Meyer, Sylke and Hatzfeld, Mechthild and Singer, Robert H. and Hüttelmaier, Stefan}, year={2006}, month=nov, pages={527–534} }

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