DOI: 10.1083/jcb.200603044. mBet3p is required for homotypic COPII vesicle tethering in mammalian cells

mBet3p is required for homotypic COPII vesicle tethering in mammalian cells

10.1083/jcb.200603044

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

TRAPPI is a large complex that mediates the tethering of COPII vesicles to the Golgi (heterotypic tethering) in the yeast Saccharomyces cerevisiae. In mammalian cells, COPII vesicles derived from the transitional endoplasmic reticulum (tER) do not tether directly to the Golgi, instead, they appear to tether to each other (homotypic tethering) to form vesicular tubular clusters (VTCs). We show that mammalian Bet3p (mBet3p), which is the most highly conserved TRAPP subunit, resides on the tER and adjacent VTCs. The inactivation of mBet3p results in the accumulation of cargo in membranes that colocalize with the COPII coat. Furthermore, using an assay that reconstitutes VTC biogenesis in vitro, we demonstrate that mBet3p is required for the tethering and fusion of COPII vesicles to each other. Consistent with the proposal that mBet3p is required for VTC biogenesis, we find that ERGIC-53 (VTC marker) and Golgi architecture are disrupted in siRNA-treated mBet3p-depleted cells. These findings imply that the TRAPPI complex is essential for VTC biogenesis.

Bibliography

Yu, S., Satoh, A., Pypaert, M., Mullen, K., Hay, J. C., & Ferro-Novick, S. (2006). mBet3p is required for homotypic COPII vesicle tethering in mammalian cells. The Journal of Cell Biology, 174(3), 359â368.

Authors 6

Sidney Yu (first)
Ayano Satoh (additional)
Marc Pypaert (additional)
Karl Mullen (additional)
Jesse C. Hay (additional)
Susan Ferro-Novick (additional)

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Dates

Type	When
Created	19 years ago (Aug. 1, 2006, 6:36 p.m.)
Deposited	2 years ago (July 29, 2023, 3:39 a.m.)
Indexed	4 months, 2 weeks ago (April 13, 2025, 6:02 a.m.)
Issued	19 years ago (July 31, 2006)
Published	19 years ago (July 31, 2006)
Published Online	19 years ago (July 31, 2006)
Published Print	19 years ago (July 31, 2006)

Funders 0

None

BibTeX

@article{Yu_2006, title={mBet3p is required for homotypic COPII vesicle tethering in mammalian cells}, volume={174}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200603044}, DOI={10.1083/jcb.200603044}, number={3}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Yu, Sidney and Satoh, Ayano and Pypaert, Marc and Mullen, Karl and Hay, Jesse C. and Ferro-Novick, Susan}, year={2006}, month=jul, pages={359–368} }

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