DOI: 10.1083/jcb.200602032. Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast

Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast

10.1083/jcb.200602032

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

We observed live fission yeast expressing pairs of functional fluorescent fusion proteins to test the popular model that the cytokinetic contractile ring assembles from a single myosin II progenitor or a Cdc12p-Cdc15p spot. Under our conditions, the anillin-like protein Mid1p establishes a broad band of small dots or nodes in the cortex near the nucleus. These nodes mature by the addition of conventional myosin II (Myo2p, Cdc4p, and Rlc1p), IQGAP (Rng2p), pombe Cdc15 homology protein (Cdc15p), and formin (Cdc12p). The nodes coalesce laterally into a compact ring when Cdc12p and profilin Cdc3p stimulate actin polymerization. We did not observe assembly of contractile rings by extension of a leading cable from a single spot or progenitor. Arp2/3 complex and its activators accumulate in patches near the contractile ring early in anaphase B, but are not concentrated in the contractile ring and are not required for assembly of the contractile ring. Their absence delays late steps in cytokinesis, including septum formation and cell separation.

Bibliography

Wu, J.-Q., Sirotkin, V., Kovar, D. R., Lord, M., Beltzner, C. C., Kuhn, J. R., & Pollard, T. D. (2006). Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast. The Journal of Cell Biology, 174(3), 391â402.

Authors 7

Jian-Qiu Wu (first)
Vladimir Sirotkin (additional)
David R. Kovar (additional)
Matthew Lord (additional)
Christopher C. Beltzner (additional)
Jeffrey R. Kuhn (additional)
Thomas D. Pollard (additional)

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Dates

Type	When
Created	19 years, 1 month ago (July 24, 2006, 8:25 p.m.)
Deposited	2 years ago (July 29, 2023, 6:29 a.m.)
Indexed	1 month, 2 weeks ago (July 11, 2025, 9:29 p.m.)
Issued	19 years, 1 month ago (July 24, 2006)
Published	19 years, 1 month ago (July 24, 2006)
Published Online	19 years, 1 month ago (July 24, 2006)
Published Print	19 years ago (July 31, 2006)

Funders 0

None

BibTeX

@article{Wu_2006, title={Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast}, volume={174}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200602032}, DOI={10.1083/jcb.200602032}, number={3}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Wu, Jian-Qiu and Sirotkin, Vladimir and Kovar, David R. and Lord, Matthew and Beltzner, Christopher C. and Kuhn, Jeffrey R. and Pollard, Thomas D.}, year={2006}, month=jul, pages={391–402} }

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