DOI: 10.1083/jcb.200510084. Nonvesicular sterol movement from plasma membrane to ER requires oxysterol-binding protein

Nonvesicular sterol movement from plasma membrane to ER requires oxysterol-binding protein–related proteins and phosphoinositides

10.1083/jcb.200510084

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Sterols are moved between cellular membranes by nonvesicular pathways whose functions are poorly understood. In yeast, one such pathway transfers sterols from the plasma membrane (PM) to the endoplasmic reticulum (ER). We show that this transport requires oxysterol-binding protein (OSBP)–related proteins (ORPs), which are a large family of conserved lipid-binding proteins. We demonstrate that a representative member of this family, Osh4p/Kes1p, specifically facilitates the nonvesicular transfer of cholesterol and ergosterol between membranes in vitro. In addition, Osh4p transfers sterols more rapidly between membranes containing phosphoinositides (PIPs), suggesting that PIPs regulate sterol transport by ORPs. We confirmed this by showing that PM to ER sterol transport slows dramatically in mutants with conditional defects in PIP biosynthesis. Our findings argue that ORPs move sterols among cellular compartments and that sterol transport and intracellular distribution are regulated by PIPs.

Bibliography

Raychaudhuri, S., Im, Y. J., Hurley, J. H., & Prinz, W. A. (2006). Nonvesicular sterol movement from plasma membrane to ER requires oxysterol-binding proteinârelated proteins and phosphoinositides. The Journal of Cell Biology, 173(1), 107â119.

Authors 4

Sumana Raychaudhuri (first)
Young Jun Im (additional)
James H. Hurley (additional)
William A. Prinz (additional)

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Dates

Type	When
Created	19 years, 4 months ago (April 3, 2006, 8:33 p.m.)
Deposited	2 years ago (July 29, 2023, 3:26 a.m.)
Indexed	1 month ago (July 26, 2025, 5:12 a.m.)
Issued	19 years, 4 months ago (April 3, 2006)
Published	19 years, 4 months ago (April 3, 2006)
Published Online	19 years, 4 months ago (April 3, 2006)
Published Print	19 years, 4 months ago (April 10, 2006)

Funders 0

None

BibTeX

@article{Raychaudhuri_2006, title={Nonvesicular sterol movement from plasma membrane to ER requires oxysterol-binding protein–related proteins and phosphoinositides}, volume={173}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200510084}, DOI={10.1083/jcb.200510084}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Raychaudhuri, Sumana and Im, Young Jun and Hurley, James H. and Prinz, William A.}, year={2006}, month=apr, pages={107–119} }

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  "abstract": "<jats:p>Sterols are moved between cellular membranes by nonvesicular pathways whose functions are poorly understood. In yeast, one such pathway transfers sterols from the plasma membrane (PM) to the endoplasmic reticulum (ER). We show that this transport requires oxysterol-binding protein (OSBP)\u2013related proteins (ORPs), which are a large family of conserved lipid-binding proteins. We demonstrate that a representative member of this family, Osh4p/Kes1p, specifically facilitates the nonvesicular transfer of cholesterol and ergosterol between membranes in vitro. In addition, Osh4p transfers sterols more rapidly between membranes containing phosphoinositides (PIPs), suggesting that PIPs regulate sterol transport by ORPs. We confirmed this by showing that PM to ER sterol transport slows dramatically in mutants with conditional defects in PIP biosynthesis. Our findings argue that ORPs move sterols among cellular compartments and that sterol transport and intracellular distribution are regulated by PIPs.</jats:p>",
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