DOI: 10.1083/jcb.200510015. Changes in chromatin structure and mobility in living cells at sites of DNA double-strand breaks

Changes in chromatin structure and mobility in living cells at sites of DNA double-strand breaks

10.1083/jcb.200510015

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The repair of DNA double-strand breaks (DSBs) is facilitated by the phosphorylation of H2AX, which organizes DNA damage signaling and chromatin remodeling complexes in the vicinity of the lesion (Pilch, D.R., O.A. Sedelnikova, C. Redon, A. Celeste, A. Nussenzweig, and W.M. Bonner. 2003. Biochem. Cell Biol. 81:123–129; Morrison, A.J., and X. Shen. 2005. Cell Cycle. 4:568–571; van Attikum, H., and S.M. Gasser. 2005. Nat. Rev. Mol. Cell. Biol. 6:757–765). The disruption of DNA integrity induces an alteration of chromatin architecture that has been proposed to activate the DNA damage transducing kinase ataxia telangiectasia mutated (ATM; Bakkenist, C.J., and M.B. Kastan. 2003. Nature. 421:499–506). However, little is known about the physical properties of damaged chromatin. In this study, we use a photoactivatable version of GFP-tagged histone H2B to examine the mobility and structure of chromatin containing DSBs in living cells. We find that chromatin containing DSBs exhibits limited mobility but undergoes an energy-dependent local expansion immediately after DNA damage. The localized expansion observed in real time corresponds to a 30–40% reduction in the density of chromatin fibers in the vicinity of DSBs, as measured by energy-filtering transmission electron microscopy. The observed opening of chromatin occurs independently of H2AX and ATM. We propose that localized adenosine triphosphate–dependent decondensation of chromatin at DSBs establishes an accessible subnuclear environment that facilitates DNA damage signaling and repair.

Bibliography

Kruhlak, M. J., Celeste, A., Dellaire, G., Fernandez-Capetillo, O., MuÌller, W. G., McNally, J. G., Bazett-Jones, D. P., & Nussenzweig, A. (2006). Changes in chromatin structure and mobility in living cells at sites of DNA double-strand breaks. The Journal of Cell Biology, 172(6), 823â834.

Authors 8

Michael J. Kruhlak (first)
Arkady Celeste (additional)
Graham Dellaire (additional)
Oscar Fernandez-Capetillo (additional)
Waltraud G. Müller (additional)
James G. McNally (additional)
David P. Bazett-Jones (additional)
André Nussenzweig (additional)

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Dates

Type	When
Created	19 years, 5 months ago (March 6, 2006, 8:25 p.m.)
Deposited	2 years, 1 month ago (July 29, 2023, 3:11 a.m.)
Indexed	1 day, 22 hours ago (Sept. 4, 2025, 9:36 a.m.)
Issued	19 years, 6 months ago (March 6, 2006)
Published	19 years, 6 months ago (March 6, 2006)
Published Online	19 years, 6 months ago (March 6, 2006)
Published Print	19 years, 5 months ago (March 13, 2006)

Funders 0

None

BibTeX

@article{Kruhlak_2006, title={Changes in chromatin structure and mobility in living cells at sites of DNA double-strand breaks}, volume={172}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200510015}, DOI={10.1083/jcb.200510015}, number={6}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Kruhlak, Michael J. and Celeste, Arkady and Dellaire, Graham and Fernandez-Capetillo, Oscar and Müller, Waltraud G. and McNally, James G. and Bazett-Jones, David P. and Nussenzweig, André}, year={2006}, month=mar, pages={823–834} }

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