DOI: 10.1083/jcb.200509095. Regulation of Sar1 NH2 terminus by GTP binding and hydrolysis promotes membrane deformation to control COPII vesicle fission

Regulation of Sar1 NH2 terminus by GTP binding and hydrolysis promotes membrane deformation to control COPII vesicle fission

10.1083/jcb.200509095

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The mechanisms by which the coat complex II (COPII) coat mediates membrane deformation and vesicle fission are unknown. Sar1 is a structural component of the membrane-binding inner layer of COPII (Bi, X., R.A. Corpina, and J. Goldberg. 2002. Nature. 419:271–277). Using model liposomes we found that Sar1 uses GTP-regulated exposure of its NH2-terminal tail, an amphipathic peptide domain, to bind, deform, constrict, and destabilize membranes. Although Sar1 activation leads to constriction of endoplasmic reticulum (ER) membranes, progression to effective vesicle fission requires a functional Sar1 NH2 terminus and guanosine triphosphate (GTP) hydrolysis. Inhibition of Sar1 GTP hydrolysis, which stabilizes Sar1 membrane binding, resulted in the formation of coated COPII vesicles that fail to detach from the ER. Thus Sar1-mediated GTP binding and hydrolysis regulates the NH2-terminal tail to perturb membrane packing, promote membrane deformation, and control vesicle fission.

Bibliography

Bielli, A., Haney, C. J., Gabreski, G., Watkins, S. C., Bannykh, S. I., & Aridor, M. (2005). Regulation of Sar1 NH2 terminus by GTP binding and hydrolysis promotes membrane deformation to control COPII vesicle fission. The Journal of Cell Biology, 171(6), 919â924.

Authors 6

Anna Bielli (first)
Charles J. Haney (additional)
Gavin Gabreski (additional)
Simon C. Watkins (additional)
Sergei I. Bannykh (additional)
Meir Aridor (additional)

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Dates

Type	When
Created	19 years, 8 months ago (Dec. 12, 2005, 8:50 p.m.)
Deposited	2 years ago (July 29, 2023, 3 a.m.)
Indexed	1 month, 2 weeks ago (July 8, 2025, 4:26 p.m.)
Issued	19 years, 8 months ago (Dec. 12, 2005)
Published	19 years, 8 months ago (Dec. 12, 2005)
Published Online	19 years, 8 months ago (Dec. 12, 2005)
Published Print	19 years, 8 months ago (Dec. 19, 2005)

Funders 0

None

BibTeX

@article{Bielli_2005, title={Regulation of Sar1 NH2 terminus by GTP binding and hydrolysis promotes membrane deformation to control COPII vesicle fission}, volume={171}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200509095}, DOI={10.1083/jcb.200509095}, number={6}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Bielli, Anna and Haney, Charles J. and Gabreski, Gavin and Watkins, Simon C. and Bannykh, Sergei I. and Aridor, Meir}, year={2005}, month=dec, pages={919–924} }

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