DOI: 10.1083/jcb.200509028. Quantitative elucidation of a distinct spatial gradient-sensing mechanism in fibroblasts

Quantitative elucidation of a distinct spatial gradient-sensing mechanism in fibroblasts

10.1083/jcb.200509028

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Migration of eukaryotic cells toward a chemoattractant often relies on their ability to distinguish receptor-mediated signaling at different subcellular locations, a phenomenon known as spatial sensing. A prominent example that is seen during wound healing is fibroblast migration in platelet-derived growth factor (PDGF) gradients. As in the well-characterized chemotactic cells Dictyostelium discoideum and neutrophils, signaling to the cytoskeleton via the phosphoinositide 3-kinase pathway in fibroblasts is spatially polarized by a PDGF gradient; however, the sensitivity of this process and how it is regulated are unknown. Through a quantitative analysis of mathematical models and live cell total internal reflection fluorescence microscopy experiments, we demonstrate that PDGF detection is governed by mechanisms that are fundamentally different from those in D. discoideum and neutrophils. Robust PDGF sensing requires steeper gradients and a much narrower range of absolute chemoattractant concentration, which is consistent with a simpler system lacking the feedback loops that yield signal amplification and adaptation in amoeboid cells.

Bibliography

Schneider, I. C., & Haugh, J. M. (2005). Quantitative elucidation of a distinct spatial gradient-sensing mechanism in fibroblasts. The Journal of Cell Biology, 171(5), 883â892.

Authors 2

Ian C. Schneider (first)
Jason M. Haugh (additional)

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Dates

Type	When
Created	19 years, 9 months ago (Nov. 28, 2005, 8:25 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 3:23 p.m.)
Indexed	2 months, 1 week ago (June 20, 2025, 6:25 p.m.)
Issued	19 years, 9 months ago (Nov. 28, 2005)
Published	19 years, 9 months ago (Nov. 28, 2005)
Published Online	19 years, 9 months ago (Nov. 28, 2005)
Published Print	19 years, 8 months ago (Dec. 5, 2005)

Funders 0

None

BibTeX

@article{Schneider_2005, title={Quantitative elucidation of a distinct spatial gradient-sensing mechanism in fibroblasts}, volume={171}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200509028}, DOI={10.1083/jcb.200509028}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Schneider, Ian C. and Haugh, Jason M.}, year={2005}, month=nov, pages={883–892} }

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