DOI: 10.1083/jcb.200508154. Repo-Man recruits PP1γ to chromatin and is essential for cell viability

Repo-Man recruits PP1γ to chromatin and is essential for cell viability

10.1083/jcb.200508154

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Protein phosphatase 1 (PP1) is a ubiquitous serine/threonine phosphatase regulating many cellular processes. PP1α and -γ are closely related isoforms with distinct localization patterns, shown here by time-lapse microscopy of stably expressed fluorescent protein fusions. A pool of PP1γ is selectively loaded onto chromatin at anaphase. Using stable isotope labeling and proteomics, we identified a novel PP1 binding protein, Repo-Man, which selectively recruits PP1γ onto mitotic chromatin at anaphase and into the following interphase. This approach revealed both novel and known PP1 binding proteins, quantitating their relative distribution between PP1α and -γ in vivo. When overexpressed, Repo-Man can also recruit PP1α to chromatin. Mutating Repo-Man's PP1 binding domain does not disrupt chromatin binding but abolishes recruitment of PP1 onto chromatin. RNA interference–induced knockdown of Repo-Man caused large-scale cell death by apoptosis, as did overexpression of this dominant-negative mutant. The data indicate that Repo-Man forms an essential complex with PP1γ and is required for the recruitment of PP1 to chromatin.

Bibliography

Trinkle-Mulcahy, L., Andersen, J., Lam, Y. W., Moorhead, G., Mann, M., & Lamond, A. I. (2006). Repo-Man recruits PP1Î³ to chromatin and is essential for cell viability. The Journal of Cell Biology, 172(5), 679â692.

Authors 6

Laura Trinkle-Mulcahy (first)
Jens Andersen (additional)
Yun Wah Lam (additional)
Greg Moorhead (additional)
Matthias Mann (additional)
Angus I. Lamond (additional)

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Dates

Type	When
Created	19 years, 6 months ago (Feb. 21, 2006, 11:04 p.m.)
Deposited	2 years ago (July 29, 2023, 3:10 a.m.)
Indexed	1 week ago (Aug. 19, 2025, 6:29 a.m.)
Issued	19 years, 6 months ago (Feb. 21, 2006)
Published	19 years, 6 months ago (Feb. 21, 2006)
Published Online	19 years, 6 months ago (Feb. 21, 2006)
Published Print	19 years, 5 months ago (Feb. 27, 2006)

Funders 0

None

BibTeX

@article{Trinkle_Mulcahy_2006, title={Repo-Man recruits PP1γ to chromatin and is essential for cell viability}, volume={172}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200508154}, DOI={10.1083/jcb.200508154}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Trinkle-Mulcahy, Laura and Andersen, Jens and Lam, Yun Wah and Moorhead, Greg and Mann, Matthias and Lamond, Angus I.}, year={2006}, month=feb, pages={679–692} }

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