DOI: 10.1083/jcb.200506199. The role of the integral membrane nucleoporins Ndc1p and Pom152p in nuclear pore complex assembly and function

The role of the integral membrane nucleoporins Ndc1p and Pom152p in nuclear pore complex assembly and function

10.1083/jcb.200506199

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The nuclear pore complex (NPC) is a large channel that spans the two lipid bilayers of the nuclear envelope and mediates transport events between the cytoplasm and the nucleus. Only a few NPC components are transmembrane proteins, and the role of these proteins in NPC function and assembly remains poorly understood. We investigate the function of the three integral membrane nucleoporins, which are Ndc1p, Pom152p, and Pom34p, in NPC assembly and transport in Saccharomyces cerevisiae. We find that Ndc1p is important for the correct localization of nuclear transport cargoes and of components of the NPC. However, the role of Ndc1p in NPC assembly is partially redundant with Pom152p, as cells lacking both of these proteins show enhanced NPC disruption. Electron microscopy studies reveal that the absence of Ndc1p and Pom152p results in aberrant pores that have enlarged diameters and lack proteinaceous material, leading to an increased diffusion between the cytoplasm and the nucleus.

Bibliography

Madrid, A. S., Mancuso, J., Cande, W. Z., & Weis, K. (2006). The role of the integral membrane nucleoporins Ndc1p and Pom152p in nuclear pore complex assembly and function. The Journal of Cell Biology, 173(3), 361â371.

Authors 4

Alexis S. Madrid (first)
Joel Mancuso (additional)
W. Zacheus Cande (additional)
Karsten Weis (additional)

References 38 Referenced 82

10.1016/j.molcel.2004.12.010 / Mol. Cell. (2005)
10.1073/pnas.252749899 / Proc. Natl. Acad. Sci. USA. (2003)
10.1083/jcb.143.7.1789 / J. Cell Biol. (1998)
10.1091/mbc.e03-04-0260 / Mol. Biol. Cell. (2003)
10.1093/emboj/20.15.4183 / EMBO J. (2001)
10.1083/jcb.200108145 / J. Cell Biol. (2002)
10.1016/S0960-9822(00)00527-3 / Curr. Biol. (2000)
10.1016/j.febslet.2004.07.044 / FEBS Lett. (2004)
10.1093/emboj/21.11.2788 / EMBO J. (2002)
10.1017/S1355838201002369 / RNA. (2001)
10.1016/0092-8674(93)90498-F / Cell. (1993)
10.1091/mbc.e03-05-0275 / Mol. Biol. Cell. (2003)
10.1016/S1097-2765(03)00116-3 / Mol. Cell. (2003)
10.1016/S1097-2765(00)80266-X / Mol. Cell. (2000)
10.1101/gad.12.17.2673 / Genes Dev. (1998)
10.1128/EC.3.2.447-458.2004 / Eukaryot. Cell. (2004)
10.1046/j.1365-2958.2002.02844.x / Mol. Microbiol. (2002)
10.1002/(SICI)1097-0061(199807)14:10<953::AID-YEA293>3.0.CO;2-U / Yeast. (1998)
10.4161/cc.3.12.1345 / Cell Cycle. (2004)
10.1016/j.molcel.2006.02.015 / Mol. Cell. (2006)
10.1083/jcb.55.2.433 / J. Cell Biol. (1972)
{'key': '2023072907253594600_BIB22', 'first-page': '77', 'volume': '117', 'year': '1999', 'journal-title': 'Methods Mol. Biol.'} / Methods Mol. Biol. (1999)
10.1128/aem.63.7.2897-2905.1997 / Appl. Environ. Microbiol. (1997)
10.1177/44.12.8985128 / J. Histochem. Cytochem. (1996)
10.1016/j.ceb.2004.04.001 / Curr. Opin. Cell Biol. (2004)
10.1083/jcb.148.4.635 / J. Cell Biol. (2000)
10.1083/jcb.200209116 / J. Cell Biol. (2003)
10.1083/jcb.149.5.1027 / J. Cell Biol. (2000)
10.1073/pnas.93.24.13641 / Proc. Natl. Acad. Sci. USA. (1996)
10.1093/genetics/122.1.19 / Genetics. (1989)
10.1016/S0092-8674(00)80981-2 / Cell. (1996)
10.1016/0092-8674(86)90485-X / Cell. (1986)
10.1016/S0092-8674(03)00235-6 / Cell. (2003)
10.1038/nature01898 / Nature. (2003)
10.1083/jcb.122.4.743 / J. Cell Biol. (1993)
10.1091/mbc.8.11.2119 / Mol. Biol. Cell. (1997)
10.1126/science.285.5429.901 / Science. (1999)
10.1126/science.288.5470.1429 / Science. (2000)

Dates

Type	When
Created	19 years, 3 months ago (May 8, 2006, 6:58 p.m.)
Deposited	2 years, 1 month ago (July 29, 2023, 3:27 a.m.)
Indexed	4 months, 1 week ago (April 24, 2025, 7:22 p.m.)
Issued	19 years, 3 months ago (May 8, 2006)
Published	19 years, 3 months ago (May 8, 2006)
Published Online	19 years, 3 months ago (May 8, 2006)
Published Print	19 years, 3 months ago (May 8, 2006)

Funders 0

None

BibTeX

@article{Madrid_2006, title={The role of the integral membrane nucleoporins Ndc1p and Pom152p in nuclear pore complex assembly and function}, volume={173}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200506199}, DOI={10.1083/jcb.200506199}, number={3}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Madrid, Alexis S. and Mancuso, Joel and Cande, W. Zacheus and Weis, Karsten}, year={2006}, month=may, pages={361–371} }

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      "first-page": "433",
      "DOI": "10.1083/jcb.55.2.433",
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      "DOI": "10.1016/j.ceb.2004.04.001",
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      "first-page": "635",
      "DOI": "10.1083/jcb.148.4.635",
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    },
    {
      "key": "2023072907253594600_BIB27",
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      "first-page": "1041",
      "DOI": "10.1083/jcb.200209116",
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      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072907253594600_BIB28",
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      "first-page": "1027",
      "DOI": "10.1083/jcb.149.5.1027",
      "volume": "149",
      "year": "2000",
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      "key": "2023072907253594600_BIB29",
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      "first-page": "13641",
      "DOI": "10.1073/pnas.93.24.13641",
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      "year": "1996",
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    {
      "key": "2023072907253594600_BIB30",
      "doi-asserted-by": "crossref",
      "first-page": "19",
      "DOI": "10.1093/genetics/122.1.19",
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    {
      "key": "2023072907253594600_BIB31",
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      "first-page": "265",
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    {
      "key": "2023072907253594600_BIB32",
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      "first-page": "65",
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    {
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      "first-page": "689",
      "DOI": "10.1038/nature01898",
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      "journal-title": "Nature."
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      "first-page": "743",
      "DOI": "10.1083/jcb.122.4.743",
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      "first-page": "2119",
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