DOI: 10.1083/jcb.200410027. In vitro assembly and GTP hydrolysis by bacterial tubulins BtubA and BtubB

In vitro assembly and GTP hydrolysis by bacterial tubulins BtubA and BtubB

10.1083/jcb.200410027

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Arecent study identified genuine tubulin proteins, BtubA and BtubB, in the bacterial genus Prosthecobacter. We have expressed BtubA and BtubB in Escherichia coli and studied their in vitro assembly. BtubB by itself formed rings with an outer diameter of 35–36 nm in the presence of GTP or GDP. Mixtures of BtubB and BtubA formed long protofilament bundles, 4–7 protofilaments wide (20–30 protofilaments in the three-dimensional bundle). Regardless of the starting stoichiometry, the polymers always contained equal concentrations of BtubA and BtubB, suggesting that BtubA and B alternate along the protofilament. BtubA showed negligible GTP hydrolysis, whereas BtubB hydrolyzed 0.40 mol GTP per min per mol BtubB. This GTPase activity increased to 1.37 per min when mixed 1:1 with BtubA. A critical concentration of 0.4–1.0 μM was indicated by light scattering experiments and extrapolation of GTPase versus concentration, thus suggesting a cooperative assembly mechanism.

Bibliography

Sontag, C. A., Staley, J. T., & Erickson, H. P. (2005). In vitro assembly and GTP hydrolysis by bacterial tubulins BtubA and BtubB. The Journal of Cell Biology, 169(2), 233â238.

Authors 3

Christopher A. Sontag (first)
James T. Staley (additional)
Harold P. Erickson (additional)

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Dates

Type	When
Created	20 years, 3 months ago (April 25, 2005, 3:04 p.m.)
Deposited	2 years ago (July 22, 2023, 1:26 p.m.)
Indexed	3 months, 2 weeks ago (May 1, 2025, 11:41 a.m.)
Issued	20 years, 3 months ago (April 25, 2005)
Published	20 years, 3 months ago (April 25, 2005)
Published Online	20 years, 3 months ago (April 25, 2005)
Published Print	20 years, 3 months ago (April 25, 2005)

Funders 0

None

BibTeX

@article{Sontag_2005, title={In vitro assembly and GTP hydrolysis by bacterial tubulins BtubA and BtubB}, volume={169}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200410027}, DOI={10.1083/jcb.200410027}, number={2}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Sontag, Christopher A. and Staley, James T. and Erickson, Harold P.}, year={2005}, month=apr, pages={233–238} }

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