DOI: 10.1083/jcb.200404173. Live cell imaging of the assembly, disassembly, and actin cable–dependent movement of endosomes and actin patches in the budding yeast, <i>Saccharomyces cerevisiae </i>

Live cell imaging of the assembly, disassembly, and actin cable–dependent movement of endosomes and actin patches in the budding yeast, Saccharomyces cerevisiae

10.1083/jcb.200404173

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Using FM4-64 to label endosomes and Abp1p-GFP or Sac6p-GFP to label actin patches, we find that (1) endosomes colocalize with actin patches as they assemble at the bud cortex; (2) endosomes colocalize with actin patches as they undergo linear, retrograde movement from buds toward mother cells; and (3) actin patches interact with and disassemble at FM4-64–labeled internal compartments. We also show that retrograde flow of actin cables mediates retrograde actin patch movement. An Arp2/3 complex mutation decreases the frequency of cortical, nonlinear actin patch movements, but has no effect on the velocity of linear, retrograde actin patch movement. Rather, linear actin patch movement occurs at the same velocity and direction as the movement of actin cables. Moreover, actin patches require actin cables for retrograde movements and colocalize with actin cables as they undergo retrograde movement. Our studies support a mechanism whereby actin cables serve as “conveyor belts” for retrograde movement and delivery of actin patches/endosomes to FM4-64–labeled internal compartments.

Bibliography

Huckaba, T. M., Gay, A. C., Pantalena, L. F., Yang, H.-C., & Pon, L. A. (2004). Live cell imaging of the assembly, disassembly, and actin cableâdependent movement of endosomes and actin patches in the budding yeast, Saccharomyces cerevisiae . The Journal of Cell Biology, 167(3), 519â530.

Authors 5

Thomas M. Huckaba (first)
Anna Card Gay (additional)
Luiz Fernando Pantalena (additional)
Hyeong-Cheol Yang (additional)
Liza A. Pon (additional)

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Dates

Type	When
Created	20 years, 9 months ago (Nov. 8, 2004, 2:18 p.m.)
Deposited	2 years ago (July 29, 2023, 2:48 a.m.)
Indexed	3 months, 1 week ago (May 12, 2025, 3:22 p.m.)
Issued	20 years, 9 months ago (Nov. 8, 2004)
Published	20 years, 9 months ago (Nov. 8, 2004)
Published Online	20 years, 9 months ago (Nov. 8, 2004)
Published Print	20 years, 9 months ago (Nov. 8, 2004)

Funders 0

None

BibTeX

@article{Huckaba_2004, title={Live cell imaging of the assembly, disassembly, and actin cable–dependent movement of endosomes and actin patches in the budding yeast, Saccharomyces cerevisiae }, volume={167}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200404173}, DOI={10.1083/jcb.200404173}, number={3}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Huckaba, Thomas M. and Gay, Anna Card and Pantalena, Luiz Fernando and Yang, Hyeong-Cheol and Pon, Liza A.}, year={2004}, month=nov, pages={519–530} }

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