DOI: 10.1083/jcb.200401085. MARK/PAR1 kinase is a regulator of microtubule-dependent transport in axons

MARK/PAR1 kinase is a regulator of microtubule-dependent transport in axons

10.1083/jcb.200401085

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Microtubule-dependent transport of vesicles and organelles appears saltatory because particles switch between periods of rest, random Brownian motion, and active transport. The transport can be regulated through motor proteins, cargo adaptors, or microtubule tracks. We report here a mechanism whereby microtubule associated proteins (MAPs) represent obstacles to motors which can be regulated by microtubule affinity regulating kinase (MARK)/Par-1, a family of kinases that is known for its involvement in establishing cell polarity and in phosphorylating tau protein during Alzheimer neurodegeneration. Expression of MARK causes the phosphorylation of MAPs at their KXGS motifs, thereby detaching MAPs from the microtubules and thus facilitating the transport of particles. This occurs without impairing the intrinsic activity of motors because the velocity during active movement remains unchanged. In primary retinal ganglion cells, transfection with tau leads to the inhibition of axonal transport of mitochondria, APP vesicles, and other cell components which leads to starvation of axons and vulnerability against stress. This transport inhibition can be rescued by phosphorylating tau with MARK.

Bibliography

Mandelkow, E.-M., Thies, E., Trinczek, B., Biernat, J., & Mandelkow, E. (2004). MARK/PAR1 kinase is a regulator of microtubule-dependent transport in axons. The Journal of Cell Biology, 167(1), 99â110.

Authors 5

Eva-Maria Mandelkow (first)
Edda Thies (additional)
Bernhard Trinczek (additional)
Jacek Biernat (additional)
Eckard Mandelkow (additional)

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Dates

Type	When
Created	20 years, 11 months ago (Oct. 4, 2004, 8:13 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 9:14 a.m.)
Indexed	2 days, 21 hours ago (Sept. 3, 2025, 6:25 a.m.)
Issued	20 years, 11 months ago (Oct. 4, 2004)
Published	20 years, 11 months ago (Oct. 4, 2004)
Published Online	20 years, 11 months ago (Oct. 4, 2004)
Published Print	20 years, 10 months ago (Oct. 11, 2004)

Funders 0

None

BibTeX

@article{Mandelkow_2004, title={MARK/PAR1 kinase is a regulator of microtubule-dependent transport in axons}, volume={167}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200401085}, DOI={10.1083/jcb.200401085}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Mandelkow, Eva-Maria and Thies, Edda and Trinczek, Bernhard and Biernat, Jacek and Mandelkow, Eckard}, year={2004}, month=oct, pages={99–110} }

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