DOI: 10.1083/jcb.200401010. VCIP135 acts as a deubiquitinating enzyme during p97–p47-mediated reassembly of mitotic Golgi fragments

VCIP135 acts as a deubiquitinating enzyme during p97–p47-mediated reassembly of mitotic Golgi fragments

10.1083/jcb.200401010

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The AAA-ATPase p97/Cdc48 functions in different cellular pathways using distinct sets of adapters and other cofactors. Together with its adaptor Ufd1–Npl4, it extracts ubiquitylated substrates from the membrane for subsequent delivery to the proteasome during ER-associated degradation. Together with its adaptor p47, on the other hand, it regulates several membrane fusion events, including reassembly of Golgi cisternae after mitosis. The finding of a ubiquitin-binding domain in p47 raises the question as to whether the ubiquitin–proteasome system is also involved in membrane fusion events. Here, we show that p97–p47-mediated reassembly of Golgi cisternae requires ubiquitin, but is not dependent on proteasome-mediated proteolysis. Instead, it requires the deubiquitinating activity of one of its cofactors, VCIP135, which reverses a ubiquitylation event that occurs during mitotic disassembly. Together, these data reveal a cycle of ubiquitylation and deubiquitination that regulates Golgi membrane dynamics during mitosis. Furthermore, they represent the first evidence for a proteasome-independent function of p97/Cdc48.

Bibliography

Wang, Y., Satoh, A., Warren, G., & Meyer, H. H. (2004). VCIP135 acts as a deubiquitinating enzyme during p97âp47-mediated reassembly of mitotic Golgi fragments. The Journal of Cell Biology, 164(7), 973â978.

Authors 4

Yanzhuang Wang (first)
Ayano Satoh (additional)
Graham Warren (additional)
Hemmo H. Meyer (additional)

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Dates

Type	When
Created	21 years, 5 months ago (March 22, 2004, 8:53 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 2:01 p.m.)
Indexed	32 minutes ago (Aug. 29, 2025, 12:54 a.m.)
Issued	21 years, 5 months ago (March 22, 2004)
Published	21 years, 5 months ago (March 22, 2004)
Published Online	21 years, 5 months ago (March 22, 2004)
Published Print	21 years, 5 months ago (March 29, 2004)

Funders 0

None

BibTeX

@article{Wang_2004, title={VCIP135 acts as a deubiquitinating enzyme during p97–p47-mediated reassembly of mitotic Golgi fragments}, volume={164}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200401010}, DOI={10.1083/jcb.200401010}, number={7}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Wang, Yanzhuang and Satoh, Ayano and Warren, Graham and Meyer, Hemmo H.}, year={2004}, month=mar, pages={973–978} }

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