DOI: 10.1083/jcb.200310139. Poly(A)<b>+</b> RNAs roam the cell nucleus and pass through speckle domains in transcriptionally active and inactive cells

Poly(A)+ RNAs roam the cell nucleus and pass through speckle domains in transcriptionally active and inactive cells

10.1083/jcb.200310139

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Many of the protein factors that play a role in nuclear export of mRNAs have been identified, but still little is known about how mRNAs are transported through the cell nucleus and which nuclear compartments are involved in mRNA transport. Using fluorescent 2'O-methyl oligoribonucleotide probes, we investigated the mobility of poly(A)+ RNA in the nucleoplasm and in nuclear speckles of U2OS cells. Quantitative analysis of diffusion using photobleaching techniques revealed that the majority of poly(A)+ RNA move throughout the nucleus, including in and out of speckles (also called SC-35 domains), which are enriched for splicing factors. Interestingly, in the presence of the transcription inhibitor 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole, the association of poly(A)+ RNA with speckles remained dynamic. Our results show that RNA movement is energy dependent and that the proportion of nuclear poly(A)+ RNA that resides in speckles is a dynamic population that transiently interacts with speckles independent of the transcriptional status of the cell. Rather than the poly(A)+ RNA within speckles serving a stable structural role, our findings support the suggestion of a more active role of these regions in nuclear RNA metabolism and/or transport.

Bibliography

Molenaar, C., Abdulle, A., Gena, A., Tanke, H. J., & Dirks, R. W. (2004). Poly(A)+ RNAs roam the cell nucleus and pass through speckle domains in transcriptionally active and inactive cells. The Journal of Cell Biology, 165(2), 191â202.

Authors 5

Chris Molenaar (first)
Abadir Abdulle (additional)
Aarti Gena (additional)
Hans J. Tanke (additional)
Roeland W. Dirks (additional)

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Dates

Type	When
Created	21 years, 4 months ago (April 26, 2004, 3:23 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 11:07 a.m.)
Indexed	1 year ago (Aug. 12, 2024, 9:15 a.m.)
Issued	21 years, 4 months ago (April 26, 2004)
Published	21 years, 4 months ago (April 26, 2004)
Published Online	21 years, 4 months ago (April 26, 2004)
Published Print	21 years, 4 months ago (April 26, 2004)

Funders 0

None

BibTeX

@article{Molenaar_2004, title={Poly(A)+ RNAs roam the cell nucleus and pass through speckle domains in transcriptionally active and inactive cells}, volume={165}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200310139}, DOI={10.1083/jcb.200310139}, number={2}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Molenaar, Chris and Abdulle, Abadir and Gena, Aarti and Tanke, Hans J. and Dirks, Roeland W.}, year={2004}, month=apr, pages={191–202} }

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