DOI: 10.1083/jcb.200309161. A<i>Salmonella</i>protein causes macrophage cell death by inducing autophagy

ASalmonellaprotein causes macrophage cell death by inducing autophagy

10.1083/jcb.200309161

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Salmonella enterica, the causative agent of food poisoning and typhoid fever, induces programmed cell death in macrophages, a process found to be dependent on a type III protein secretion system, and SipB, a protein with membrane fusion activity that is delivered into host cells by this system. When expressed in cultured cells, SipB caused the formation of and localized to unusual multimembrane structures. These structures resembled autophagosomes and contained both mitochondrial and endoplasmic reticulum markers. A mutant form of SipB devoid of membrane fusion activity localized to mitochondria, but did not induce the formation of membrane structures. Upon Salmonella infection of macrophages, SipB was found in mitochondria, which appeared swollen and devoid of christae. Salmonella-infected macrophages exhibited marked accumulation of autophagic vesicles. We propose that Salmonella, through the action of SipB, kills macrophages by disrupting mitochondria, thereby inducing autophagy and cell death.

Bibliography

Hernandez, L. D., Pypaert, M., Flavell, R. A., & GalaÌn, J. E. (2003). ASalmonellaprotein causes macrophage cell death by inducing autophagy. The Journal of Cell Biology, 163(5), 1123â1131.

Authors 4

Lorraine D. Hernandez (first)
Marc Pypaert (additional)
Richard A. Flavell (additional)
Jorge E. Galán (additional)

References 42 Referenced 183

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Dates

Type	When
Created	21 years, 8 months ago (Dec. 8, 2003, 3:07 p.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 11:28 a.m.)
Indexed	1 month, 1 week ago (July 25, 2025, 6:49 a.m.)
Issued	21 years, 8 months ago (Dec. 8, 2003)
Published	21 years, 8 months ago (Dec. 8, 2003)
Published Online	21 years, 8 months ago (Dec. 8, 2003)
Published Print	21 years, 8 months ago (Dec. 8, 2003)

Funders 0

None

BibTeX

@article{Hernandez_2003, title={ASalmonellaprotein causes macrophage cell death by inducing autophagy}, volume={163}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200309161}, DOI={10.1083/jcb.200309161}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Hernandez, Lorraine D. and Pypaert, Marc and Flavell, Richard A. and Galán, Jorge E.}, year={2003}, month=dec, pages={1123–1131} }

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      }
    ]
  },
  "ISSN": [
    "1540-8140",
    "0021-9525"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        2003,
        12,
        8
      ]
    ]
  }
}