DOI: 10.1083/jcb.200308144. ADF/cofilin use an intrinsic mode of F-actin instability to disrupt actin filaments

ADF/cofilin use an intrinsic mode of F-actin instability to disrupt actin filaments

10.1083/jcb.200308144

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Proteins in the ADF/cofilin (AC) family are essential for rapid rearrangements of cellular actin structures. They have been shown to be active in both the severing and depolymerization of actin filaments in vitro, but the detailed mechanism of action is not known. Under in vitro conditions, subunits in the actin filament can treadmill; with the hydrolysis of ATP driving the addition of subunits at one end of the filament and loss of subunits from the opposite end. We have used electron microscopy and image analysis to show that AC molecules effectively disrupt one of the longitudinal contacts between protomers within one helical strand of F-actin. We show that in the absence of any AC proteins, this same longitudinal contact between actin protomers is disrupted at the depolymerizing (pointed) end of actin filaments but is prominent at the polymerizing (barbed) end. We suggest that AC proteins use an intrinsic mechanism of F-actin's internal instability to depolymerize/sever actin filaments in the cell.

Bibliography

Galkin, V. E., Orlova, A., VanLoock, M. S., Shvetsov, A., Reisler, E., & Egelman, E. H. (2003). ADF/cofilin use an intrinsic mode of F-actin instability to disrupt actin filaments. The Journal of Cell Biology, 163(5), 1057â1066.

Authors 6

Vitold E. Galkin (first)
Albina Orlova (additional)
Margaret S. VanLoock (additional)
Alexander Shvetsov (additional)
Emil Reisler (additional)
Edward H. Egelman (additional)

References 59 Referenced 91

10.1083/jcb.132.5.871 / J. Cell Biol. (1996)
10.1146/annurev.cellbio.15.1.185 / Annu. Rev. Cell Dev. Biol. (1999)
10.1073/pnas.96.1.29 / Proc. Nat. Acad. Sci. USA. (1999)
10.1046/j.0014-2956.2001.02592.x / Eur. J. Biochem. (2001)
10.1016/S0022-2836(02)01008-2 / J. Mol. Biol. (2002)
10.1002/1097-0134(20001115)41:3<374::AID-PROT90>3.0.CO;2-F / Proteins. (2000)
10.1006/jmbi.1994.1617 / J. Mol. Biol. (1994)
10.1016/S0955-0674(98)80085-9 / Curr. Opin. Cell Biol. (1998)
10.1006/jmbi.1997.1062 / J. Mol. Biol. (1997)
10.1016/S0021-9258(18)90914-2 / J. Biol. Chem. (1984)
10.1083/jcb.136.6.1307 / J. Cell Biol. (1997)
10.1016/S0021-9258(18)88819-6 / J. Biol. Chem. (1985)
10.1006/jmbi.1996.0602 / J. Mol. Biol. (1996)
10.1083/jcb.97.5.1629 / J. Cell Biol. (1983)
10.1016/S0021-9258(19)77854-5 / J. Biol. Chem. (1988)
10.1016/S0021-9258(17)42495-1 / J. Biol. Chem. (1986)
10.1016/S0006-3495(02)75655-5 / Biophys. J. (2002)
10.1016/S0304-3991(00)00062-0 / Ultramicroscopy. (2000)
10.1016/S0960-9822(01)00615-7 / Curr. Biol. (2001)
10.1038/nrm1176 / Nat. Rev. Mol. Cell Biol. (2003)
10.1016/S0959-440X(03)00027-7 / Curr. Opin. Struct. Biol. (2003)
10.1006/jsbi.1996.0030 / J. Struct. Biol. (1996)
10.1038/ncb841 / Nat. Cell Biol. (2002)
10.1083/jcb.153.1.75 / J. Cell Biol. (2001)
10.1016/S0960-9822(02)00742-X / Curr. Biol. (2002)
10.1016/S0022-2836(03)00842-8 / J. Mol. Biol. (2003)
10.1107/S0907444900009549 / Acta Crystallogr. D Biol. Crystallogr. (2000)
{'key': '2023072214473130600_BIB28', 'first-page': '146', 'volume': '73', 'year': '1986', 'journal-title': 'Optik'} / Optik (1986)
10.1038/347044a0 / Nature. (1990)
10.1107/S0108767390010224 / Acta Crystallogr. A. (1991)
10.1006/jmbi.2000.3727 / J. Mol. Biol. (2000)
10.1038/40418 / Nature. (1997)
10.1016/0014-5793(94)00552-4 / FEBS Lett. (1994)
10.1083/jcb.115.6.1611 / J. Cell Biol. (1991)
10.1046/j.1432-1327.1998.2560388.x / Eur. J. Biochem. (1998)
10.1006/jmbi.1999.2968 / J. Mol. Biol. (1999)
10.1083/jcb.138.4.771 / J. Cell Biol. (1997)
10.1038/364685a0 / Nature. (1993)
10.1074/jbc.M007563200 / J. Biol. Chem. (2001)
10.1006/jmbi.1993.1393 / J. Mol. Biol. (1993)
10.1006/jmbi.1994.0048 / J. Mol. Biol. (1995)
10.1006/jmbi.1994.0049 / J. Mol. Biol. (1995)
10.1006/jmbi.2001.4945 / J. Mol. Biol. (2001)
10.1126/science.1059700 / Science. (2001)
10.1083/jcb.123.2.337 / J. Cell Biol. (1993)
10.1021/bi9527045 / Biochemistry. (1996)
10.1006/jmbi.2000.3688 / J. Mol. Biol. (2000)
10.1074/jbc.273.33.20894 / J. Biol. Chem. (1998)
10.1073/pnas.152329899 / Proc. Natl. Acad. Sci. USA. (2002)
10.1021/bi00221a034 / Biochemistry. (1991)
10.1038/365810a0 / Nature. (1993)
10.1016/S1047-8477(03)00029-7 / J. Struct. Biol. (2003)
{'key': '2023072214473130600_BIB53', 'first-page': '1', 'volume': '2', 'year': '1995', 'journal-title': 'Protein Profile.'} / Protein Profile. (1995)
10.1016/S0022-2836(76)80100-3 / J. Mol. Biol. (1976)
{'key': '2023072214473130600_BIB55', 'volume': 'In press', 'year': '2003', 'journal-title': 'J. Struct. Biol.'} / J. Struct. Biol. (2003)
10.1006/jmbi.2001.5280 / J. Mol. Biol. (2002)
10.1016/S0021-9258(17)38580-0 / J. Biol. Chem. (1985)
10.1016/S0022-2836(03)00098-6 / J. Mol. Biol. (2003)
10.1016/S1047-8477(02)00546-4 / J. Struct. Biol. (2003)

Dates

Type	When
Created	21 years, 8 months ago (Dec. 1, 2003, 8:14 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 10:48 a.m.)
Indexed	3 months, 3 weeks ago (May 6, 2025, 1:08 p.m.)
Issued	21 years, 8 months ago (Dec. 1, 2003)
Published	21 years, 8 months ago (Dec. 1, 2003)
Published Online	21 years, 8 months ago (Dec. 1, 2003)
Published Print	21 years, 8 months ago (Dec. 8, 2003)

Funders 0

None

BibTeX

@article{Galkin_2003, title={ADF/cofilin use an intrinsic mode of F-actin instability to disrupt actin filaments}, volume={163}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200308144}, DOI={10.1083/jcb.200308144}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Galkin, Vitold E. and Orlova, Albina and VanLoock, Margaret S. and Shvetsov, Alexander and Reisler, Emil and Egelman, Edward H.}, year={2003}, month=dec, pages={1057–1066} }

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      "DOI": "10.1146/annurev.cellbio.15.1.185",
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      "first-page": "29",
      "DOI": "10.1073/pnas.96.1.29",
      "volume": "96",
      "year": "1999",
      "journal-title": "Proc. Nat. Acad. Sci. USA."
    },
    {
      "key": "2023072214473130600_BIB4",
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      "first-page": "6426",
      "DOI": "10.1046/j.0014-2956.2001.02592.x",
      "volume": "268",
      "year": "2001",
      "journal-title": "Eur. J. Biochem."
    },
    {
      "key": "2023072214473130600_BIB5",
      "doi-asserted-by": "crossref",
      "first-page": "739",
      "DOI": "10.1016/S0022-2836(02)01008-2",
      "volume": "323",
      "year": "2002",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "2023072214473130600_BIB6",
      "doi-asserted-by": "crossref",
      "first-page": "374",
      "DOI": "10.1002/1097-0134(20001115)41:3<374::AID-PROT90>3.0.CO;2-F",
      "volume": "41",
      "year": "2000",
      "journal-title": "Proteins."
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    {
      "key": "2023072214473130600_BIB7",
      "doi-asserted-by": "crossref",
      "first-page": "683",
      "DOI": "10.1006/jmbi.1994.1617",
      "volume": "242",
      "year": "1994",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "2023072214473130600_BIB8",
      "doi-asserted-by": "crossref",
      "first-page": "45",
      "DOI": "10.1016/S0955-0674(98)80085-9",
      "volume": "10",
      "year": "1998",
      "journal-title": "Curr. Opin. Cell Biol."
    },
    {
      "key": "2023072214473130600_BIB9",
      "doi-asserted-by": "crossref",
      "first-page": "459",
      "DOI": "10.1006/jmbi.1997.1062",
      "volume": "269",
      "year": "1997",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "2023072214473130600_BIB10",
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      "first-page": "9983",
      "DOI": "10.1016/S0021-9258(18)90914-2",
      "volume": "259",
      "year": "1984",
      "journal-title": "J. Biol. Chem."
    },
    {
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      "first-page": "1307",
      "DOI": "10.1083/jcb.136.6.1307",
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    {
      "key": "2023072214473130600_BIB12",
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      "first-page": "6565",
      "DOI": "10.1016/S0021-9258(18)88819-6",
      "volume": "260",
      "year": "1985",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2023072214473130600_BIB13",
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      "first-page": "607",
      "DOI": "10.1006/jmbi.1996.0602",
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      "year": "1996",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "2023072214473130600_BIB14",
      "doi-asserted-by": "crossref",
      "first-page": "1629",
      "DOI": "10.1083/jcb.97.5.1629",
      "volume": "97",
      "year": "1983",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072214473130600_BIB15",
      "doi-asserted-by": "crossref",
      "first-page": "17429",
      "DOI": "10.1016/S0021-9258(19)77854-5",
      "volume": "263",
      "year": "1988",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2023072214473130600_BIB16",
      "doi-asserted-by": "crossref",
      "first-page": "477",
      "DOI": "10.1016/S0021-9258(17)42495-1",
      "volume": "261",
      "year": "1986",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2023072214473130600_BIB17",
      "doi-asserted-by": "crossref",
      "first-page": "3134",
      "DOI": "10.1016/S0006-3495(02)75655-5",
      "volume": "82",
      "year": "2002",
      "journal-title": "Biophys. J."
    },
    {
      "key": "2023072214473130600_BIB18",
      "doi-asserted-by": "crossref",
      "first-page": "225",
      "DOI": "10.1016/S0304-3991(00)00062-0",
      "volume": "85",
      "year": "2000",
      "journal-title": "Ultramicroscopy."
    },
    {
      "key": "2023072214473130600_BIB19",
      "doi-asserted-by": "crossref",
      "first-page": "R1022",
      "DOI": "10.1016/S0960-9822(01)00615-7",
      "volume": "11",
      "year": "2001",
      "journal-title": "Curr. Biol."
    },
    {
      "key": "2023072214473130600_BIB20",
      "doi-asserted-by": "crossref",
      "first-page": "621",
      "DOI": "10.1038/nrm1176",
      "volume": "4",
      "year": "2003",
      "journal-title": "Nat. Rev. Mol. Cell Biol."
    },
    {
      "key": "2023072214473130600_BIB21",
      "doi-asserted-by": "crossref",
      "first-page": "244",
      "DOI": "10.1016/S0959-440X(03)00027-7",
      "volume": "13",
      "year": "2003",
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    {
      "key": "2023072214473130600_BIB22",
      "doi-asserted-by": "crossref",
      "first-page": "190",
      "DOI": "10.1006/jsbi.1996.0030",
      "volume": "116",
      "year": "1996",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "2023072214473130600_BIB23",
      "doi-asserted-by": "crossref",
      "first-page": "666",
      "DOI": "10.1038/ncb841",
      "volume": "4",
      "year": "2002",
      "journal-title": "Nat. Cell Biol."
    },
    {
      "key": "2023072214473130600_BIB24",
      "doi-asserted-by": "crossref",
      "first-page": "75",
      "DOI": "10.1083/jcb.153.1.75",
      "volume": "153",
      "year": "2001",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072214473130600_BIB25",
      "doi-asserted-by": "crossref",
      "first-page": "570",
      "DOI": "10.1016/S0960-9822(02)00742-X",
      "volume": "12",
      "year": "2002",
      "journal-title": "Curr. Biol."
    },
    {
      "key": "2023072214473130600_BIB26",
      "doi-asserted-by": "crossref",
      "first-page": "967",
      "DOI": "10.1016/S0022-2836(03)00842-8",
      "volume": "331",
      "year": "2003",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "2023072214473130600_BIB27",
      "doi-asserted-by": "crossref",
      "first-page": "1270",
      "DOI": "10.1107/S0907444900009549",
      "volume": "56",
      "year": "2000",
      "journal-title": "Acta Crystallogr. D Biol. Crystallogr."
    },
    {
      "key": "2023072214473130600_BIB28",
      "first-page": "146",
      "volume": "73",
      "year": "1986",
      "journal-title": "Optik"
    },
    {
      "key": "2023072214473130600_BIB29",
      "doi-asserted-by": "crossref",
      "first-page": "44",
      "DOI": "10.1038/347044a0",
      "volume": "347",
      "year": "1990",
      "journal-title": "Nature."
    },
    {
      "key": "2023072214473130600_BIB30",
      "doi-asserted-by": "crossref",
      "first-page": "110",
      "DOI": "10.1107/S0108767390010224",
      "volume": "47",
      "year": "1991",
      "journal-title": "Acta Crystallogr. A."
    },
    {
      "key": "2023072214473130600_BIB31",
      "doi-asserted-by": "crossref",
      "first-page": "421",
      "DOI": "10.1006/jmbi.2000.3727",
      "volume": "299",
      "year": "2000",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "2023072214473130600_BIB32",
      "doi-asserted-by": "crossref",
      "first-page": "78",
      "DOI": "10.1038/40418",
      "volume": "388",
      "year": "1997",
      "journal-title": "Nature."
    },
    {
      "key": "2023072214473130600_BIB33",
      "doi-asserted-by": "crossref",
      "first-page": "251",
      "DOI": "10.1016/0014-5793(94)00552-4",
      "volume": "347",
      "year": "1994",
      "journal-title": "FEBS Lett."
    },
    {
      "key": "2023072214473130600_BIB34",
      "doi-asserted-by": "crossref",
      "first-page": "1611",
      "DOI": "10.1083/jcb.115.6.1611",
      "volume": "115",
      "year": "1991",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072214473130600_BIB35",
      "doi-asserted-by": "crossref",
      "first-page": "388",
      "DOI": "10.1046/j.1432-1327.1998.2560388.x",
      "volume": "256",
      "year": "1998",
      "journal-title": "Eur. J. Biochem."
    },
    {
      "key": "2023072214473130600_BIB36",
      "doi-asserted-by": "crossref",
      "first-page": "513",
      "DOI": "10.1006/jmbi.1999.2968",
      "volume": "291",
      "year": "1999",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "2023072214473130600_BIB37",
      "doi-asserted-by": "crossref",
      "first-page": "771",
      "DOI": "10.1083/jcb.138.4.771",
      "volume": "138",
      "year": "1997",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072214473130600_BIB38",
      "doi-asserted-by": "crossref",
      "first-page": "685",
      "DOI": "10.1038/364685a0",
      "volume": "364",
      "year": "1993",
      "journal-title": "Nature."
    },
    {
      "key": "2023072214473130600_BIB39",
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      "first-page": "5952",
      "DOI": "10.1074/jbc.M007563200",
      "volume": "276",
      "year": "2001",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2023072214473130600_BIB40",
      "doi-asserted-by": "crossref",
      "first-page": "334",
      "DOI": "10.1006/jmbi.1993.1393",
      "volume": "232",
      "year": "1993",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "2023072214473130600_BIB41",
      "doi-asserted-by": "crossref",
      "first-page": "582",
      "DOI": "10.1006/jmbi.1994.0048",
      "volume": "245",
      "year": "1995",
      "journal-title": "J. Mol. Biol."
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      "key": "2023072214473130600_BIB43",
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      "first-page": "95",
      "DOI": "10.1006/jmbi.2001.4945",
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      "year": "2001",
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      "key": "2023072214473130600_BIB44",
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      "first-page": "708",
      "DOI": "10.1126/science.1059700",
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    {
      "key": "2023072214473130600_BIB45",
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      "first-page": "337",
      "DOI": "10.1083/jcb.123.2.337",
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      "year": "1993",
      "journal-title": "J. Cell Biol."
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      "key": "2023072214473130600_BIB46",
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      "first-page": "4396",
      "DOI": "10.1021/bi9527045",
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      "key": "2023072214473130600_BIB47",
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      "first-page": "649",
      "DOI": "10.1006/jmbi.2000.3688",
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      "key": "2023072214473130600_BIB48",
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      "first-page": "20894",
      "DOI": "10.1074/jbc.273.33.20894",
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      "year": "1998",
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      "key": "2023072214473130600_BIB49",
      "doi-asserted-by": "crossref",
      "first-page": "10945",
      "DOI": "10.1073/pnas.152329899",
      "volume": "99",
      "year": "2002",
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    {
      "key": "2023072214473130600_BIB50",
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      "first-page": "301",
      "DOI": "10.1016/S1047-8477(03)00029-7",
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      "year": "2003",
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      "key": "2023072214473130600_BIB53",
      "first-page": "1",
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      "first-page": "139",
      "DOI": "10.1016/S0022-2836(76)80100-3",
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      "key": "2023072214473130600_BIB55",
      "volume": "In press",
      "year": "2003",
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      "first-page": "911",
      "DOI": "10.1006/jmbi.2001.5280",
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      "first-page": "14410",
      "DOI": "10.1016/S0021-9258(17)38580-0",
      "volume": "260",
      "year": "1985",
      "journal-title": "J. Biol. Chem."
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      "first-page": "145",
      "DOI": "10.1016/S0022-2836(03)00098-6",
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      "DOI": "10.1016/S1047-8477(02)00546-4",
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