DOI: 10.1083/jcb.200307067. Functional interaction of chloroplast SRP/FtsY with the ALB3 translocase in thylakoids

Functional interaction of chloroplast SRP/FtsY with the ALB3 translocase in thylakoids

10.1083/jcb.200307067

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Integration of thylakoid proteins by the chloroplast signal recognition particle (cpSRP) posttranslational transport pathway requires the cpSRP, an SRP receptor homologue (cpFtsY), and the membrane protein ALB3. Similarly, Escherichia coli uses an SRP and FtsY to cotranslationally target membrane proteins to the SecYEG translocase, which contains an ALB3 homologue, YidC. In neither system are the interactions between soluble and membrane components well understood. We show that complexes containing cpSRP, cpFtsY, and ALB3 can be precipitated using affinity tags on cpSRP or cpFtsY. Stabilization of this complex with GMP-PNP specifically blocks subsequent integration of substrate (light harvesting chl a/b-binding protein [LHCP]), indicating that the complex occupies functional ALB3 translocation sites. Surprisingly, neither substrate nor cpSRP43, a component of cpSRP, was necessary to form a complex with ALB3. Complexes also contained cpSecY, but its removal did not inhibit ALB3 function. Furthermore, antibody bound to ALB3 prevented ALB3 association with cpSRP and cpFtsY and inhibited LHCP integration suggesting that a complex containing cpSRP, cpFtsY, and ALB3 must form for proper LHCP integration.

Bibliography

Moore, M., Goforth, R. L., Mori, H., & Henry, R. (2003). Functional interaction of chloroplast SRP/FtsY with the ALB3 translocase in thylakoids. The Journal of Cell Biology, 162(7), 1245â1254.

Authors 4

Misty Moore (first)
Robyn L. Goforth (additional)
Hiroki Mori (additional)
Ralph Henry (additional)

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Dates

Type	When
Created	21 years, 11 months ago (Sept. 29, 2003, 4:23 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 10:30 a.m.)
Indexed	3 months, 1 week ago (May 26, 2025, 4:43 a.m.)
Issued	21 years, 11 months ago (Sept. 29, 2003)
Published	21 years, 11 months ago (Sept. 29, 2003)
Published Online	21 years, 11 months ago (Sept. 29, 2003)
Published Print	21 years, 11 months ago (Sept. 29, 2003)

Funders 0

None

BibTeX

@article{Moore_2003, title={Functional interaction of chloroplast SRP/FtsY with the ALB3 translocase in thylakoids}, volume={162}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200307067}, DOI={10.1083/jcb.200307067}, number={7}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Moore, Misty and Goforth, Robyn L. and Mori, Hiroki and Henry, Ralph}, year={2003}, month=sep, pages={1245–1254} }

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