DOI: 10.1083/jcb.200306020. Formation of stacked ER cisternae by low affinity protein interactions

Formation of stacked ER cisternae by low affinity protein interactions

10.1083/jcb.200306020

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The endoplasmic reticulum (ER) can transform from a network of branching tubules into stacked membrane arrays (termed organized smooth ER [OSER]) in response to elevated levels of specific resident proteins, such as cytochrome b(5). Here, we have tagged OSER-inducing proteins with green fluorescent protein (GFP) to study OSER biogenesis and dynamics in living cells. Overexpression of these proteins induced formation of karmellae, whorls, and crystalloid OSER structures. Photobleaching experiments revealed that OSER-inducing proteins were highly mobile within OSER structures and could exchange between OSER structures and surrounding reticular ER. This indicated that binding interactions between proteins on apposing stacked membranes of OSER structures were not of high affinity. Addition of GFP, which undergoes low affinity, antiparallel dimerization, to the cytoplasmic domains of non–OSER-inducing resident ER proteins was sufficient to induce OSER structures when overexpressed, but addition of a nondimerizing GFP variant was not. These results point to a molecular mechanism for OSER biogenesis that involves weak homotypic interactions between cytoplasmic domains of proteins. This mechanism may underlie the formation of other stacked membrane structures within cells.

Bibliography

Snapp, E. L., Hegde, R. S., Francolini, M., Lombardo, F., Colombo, S., Pedrazzini, E., Borgese, N., & Lippincott-Schwartz, J. (2003). Formation of stacked ER cisternae by low affinity protein interactions. The Journal of Cell Biology, 163(2), 257â269.

Authors 8

Erik L. Snapp (first)
Ramanujan S. Hegde (additional)
Maura Francolini (additional)
Francesca Lombardo (additional)
Sara Colombo (additional)
Emanuela Pedrazzini (additional)
Nica Borgese (additional)
Jennifer Lippincott-Schwartz (additional)

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Dates

Type	When
Created	21 years, 10 months ago (Oct. 28, 2003, 2:13 a.m.)
Deposited	2 years, 1 month ago (July 29, 2023, 1:29 a.m.)
Indexed	1 week, 5 days ago (Aug. 22, 2025, 12:45 a.m.)
Issued	21 years, 10 months ago (Oct. 27, 2003)
Published	21 years, 10 months ago (Oct. 27, 2003)
Published Online	21 years, 10 months ago (Oct. 27, 2003)
Published Print	21 years, 10 months ago (Oct. 27, 2003)

Funders 0

None

BibTeX

@article{Snapp_2003, title={Formation of stacked ER cisternae by low affinity protein interactions}, volume={163}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200306020}, DOI={10.1083/jcb.200306020}, number={2}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Snapp, Erik L. and Hegde, Ramanujan S. and Francolini, Maura and Lombardo, Francesca and Colombo, Sara and Pedrazzini, Emanuela and Borgese, Nica and Lippincott-Schwartz, Jennifer}, year={2003}, month=oct, pages={257–269} }

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