DOI: 10.1083/jcb.200304128. Smn, the spinal muscular atrophy–determining gene product, modulates axon growth and localization of β-actin mRNA in growth cones of motoneurons

Smn, the spinal muscular atrophy–determining gene product, modulates axon growth and localization of β-actin mRNA in growth cones of motoneurons

10.1083/jcb.200304128

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Spinal muscular atrophy (SMA), a common autosomal recessive form of motoneuron disease in infants and young adults, is caused by mutations in the survival motoneuron 1 (SMN1) gene. The corresponding gene product is part of a multiprotein complex involved in the assembly of spliceosomal small nuclear ribonucleoprotein complexes. It is still not understood why reduced levels of the ubiquitously expressed SMN protein specifically cause motoneuron degeneration. Here, we show that motoneurons isolated from an SMA mouse model exhibit normal survival, but reduced axon growth. Overexpression of Smn or its binding partner, heterogeneous nuclear ribonucleoprotein (hnRNP) R, promotes neurite growth in differentiating PC12 cells. Reduced axon growth in Smn-deficient motoneurons correlates with reduced β-actin protein and mRNA staining in distal axons and growth cones. We also show that hnRNP R associates with the 3′ UTR of β-actin mRNA. Together, these data suggest that a complex of Smn with its binding partner hnRNP R interacts with β-actin mRNA and translocates to axons and growth cones of motoneurons.

Bibliography

Rossoll, W., Jablonka, S., Andreassi, C., KroÌning, A.-K., Karle, K., Monani, U. R., & Sendtner, M. (2003). Smn, the spinal muscular atrophyâdetermining gene product, modulates axon growth and localization of Î²-actin mRNA in growth cones of motoneurons. The Journal of Cell Biology, 163(4), 801â812.

Authors 7

Wilfried Rossoll (first)
Sibylle Jablonka (additional)
Catia Andreassi (additional)
Ann-Kathrin Kröning (additional)
Kathrin Karle (additional)
Umrao R. Monani (additional)
Michael Sendtner (additional)

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Dates

Type	When
Created	21 years, 9 months ago (Nov. 17, 2003, 8:23 p.m.)
Deposited	2 years ago (July 22, 2023, 10:47 a.m.)
Indexed	2 weeks, 5 days ago (Aug. 3, 2025, 12:07 a.m.)
Issued	21 years, 9 months ago (Nov. 17, 2003)
Published	21 years, 9 months ago (Nov. 17, 2003)
Published Online	21 years, 9 months ago (Nov. 17, 2003)
Published Print	21 years, 8 months ago (Nov. 24, 2003)

Funders 0

None

BibTeX

@article{Rossoll_2003, title={Smn, the spinal muscular atrophy–determining gene product, modulates axon growth and localization of β-actin mRNA in growth cones of motoneurons}, volume={163}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200304128}, DOI={10.1083/jcb.200304128}, number={4}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Rossoll, Wilfried and Jablonka, Sibylle and Andreassi, Catia and Kröning, Ann-Kathrin and Karle, Kathrin and Monani, Umrao R. and Sendtner, Michael}, year={2003}, month=nov, pages={801–812} }

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