DOI: 10.1083/jcb.200304096. Inheritance of gene density–related higher order chromatin arrangements in normal and tumor cell nuclei

Inheritance of gene density–related higher order chromatin arrangements in normal and tumor cell nuclei

10.1083/jcb.200304096

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

A gene density–related difference in the radial arrangement of chromosome territories (CTs) was previously described for human lymphocyte nuclei with gene-poor CT #18 located toward the nuclear periphery and gene-dense CT #19 in the nuclear interior (Croft, J.A., J.M. Bridger, S. Boyle, P. Perry, P. Teague, and W.A. Bickmore. 1999. J. Cell Biol. 145:1119–1131). Here, we analyzed the radial distribution of chromosome 18 and 19 chromatin in six normal cell types and in eight tumor cell lines, some of them with imbalances and rearrangements of the two chromosomes. Our findings demonstrate that a significant difference in the radial distribution of #18 and #19 chromatin is a common feature of higher order chromatin architecture in both normal and malignant cell types. However, in seven of eight tumor cell lines, the difference was less pronounced compared with normal cell nuclei due to a higher fraction of nuclei showing an inverted CT position, i.e., a CT #18 located more internally than a CT #19. This observation emphasizes a partial loss of radial chromatin order in tumor cell nuclei.

Bibliography

Cremer, M., KuÌpper, K., Wagler, B., Wizelman, L., Hase, J. v., Weiland, Y., Kreja, L., Diebold, J., Speicher, M. R., & Cremer, T. (2003). Inheritance of gene densityârelated higher order chromatin arrangements in normal and tumor cell nuclei. The Journal of Cell Biology, 162(5), 809â820.

Authors 10

Marion Cremer (first)
Katrin Küpper (additional)
Babett Wagler (additional)
Leah Wizelman (additional)
Johann v. Hase (additional)
Yanina Weiland (additional)
Ludwika Kreja (additional)
Joachim Diebold (additional)
Michael R. Speicher (additional)
Thomas Cremer (additional)

References 38 Referenced 208

10.1073/pnas.041603298 / Proc. Natl. Acad. Sci. USA. (2001)
10.1093/carcin/21.3.469 / Carcinogenesis. (2000)
10.1007/BF02616180 / Metrika (1962)
10.1242/jcs.77.1.167 / J. Cell Sci. (1985)
10.1093/hmg/10.3.211 / Hum. Mol. Genet. (2001)
10.1016/S0960-9822(00)00312-2 / Curr. Biol. (2000)
10.1038/32688 / Nature. (1998)
10.1016/S0165-0270(98)00066-1 / J. Neurosci. Methods. (1998)
10.1023/A:1012495201697 / Chromosome Res. (2001)
10.1083/jcb.145.6.1119 / J. Cell Biol. (1999)
10.1242/jcs.00087 / J. Cell Sci. (2002)
10.1007/BF00202476 / Hum. Genet. (1993)
10.1155/2000/502753 / Anal. Cell. Pathol. (2000)
10.1016/S0092-8674(03)00189-2 / Cell. (2003)
10.1016/0268-960X(93)90005-O / Blood Rev. (1993)
10.1023/A:1012447318535 / Chromosome Res. (2001)
10.1016/0014-4827(88)90271-6 / Exp. Cell Res. (1988)
10.1073/pnas.89.18.8611 / Proc. Natl. Acad. Sci. USA. (1992)
10.1002/eji.1830111015 / Eur. J. Immunol. (1981)
10.1002/ijc.10845 / Int. J. Cancer. (2003)
10.1016/S0960-9822(98)70156-3 / Curr. Biol. (1998)
10.1002/1096-9896(2000)9999:9999<::AID-PATH708>3.0.CO;2-I / J. Pathol. (2000)
10.1007/s00412-002-0210-8 / Chromosoma. (2002)
10.1002/gcc.10160 / Genes Chromosomes Cancer. (2003)
10.1182/blood.V87.1.1.1 / Blood. (1996)
10.1093/annonc/13.S1.11 / Ann. Oncol. (2002)
10.1016/S0955-0674(02)00335-6 / Curr. Opin. Cell Biol. (2002)
{'key': '2023072214283798500_BIB28', 'first-page': '4562', 'volume': '36', 'year': '1976', 'journal-title': 'Cancer Res.'} / Cancer Res. (1976)
10.1111/j.1432-0436.1983.tb01316.x / Differentiation. (1983)
10.1016/S0960-9822(02)01166-1 / Curr. Biol. (2002)
10.2144/99272rr04 / Biotechniques. (1999)
10.1002/ijc.2910190505 / Int. J. Cancer. (1977)
10.1177/002215549904700601 / J. Histochem. Cytochem. (1999)
10.1023/A:1009267605580 / Chromosome Res. (2000)
{'key': '2023072214283798500_BIB35'}
10.1016/S0027-5107(02)00077-5 / Mutat. Res. (2002)
10.1073/pnas.072618599 / Proc. Natl. Acad. Sci. USA. (2002)
10.1023/A:1025016828544 / Chromosome Res. (2003)

Dates

Type	When
Created	21 years, 11 months ago (Sept. 2, 2003, 4:37 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 10:29 a.m.)
Indexed	1 day, 10 hours ago (Aug. 31, 2025, 6:14 a.m.)
Issued	22 years ago (Sept. 1, 2003)
Published	22 years ago (Sept. 1, 2003)
Published Online	21 years, 11 months ago (Sept. 2, 2003)
Published Print	22 years ago (Sept. 1, 2003)

Funders 0

None

BibTeX

@article{Cremer_2003, title={Inheritance of gene density–related higher order chromatin arrangements in normal and tumor cell nuclei}, volume={162}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200304096}, DOI={10.1083/jcb.200304096}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Cremer, Marion and Küpper, Katrin and Wagler, Babett and Wizelman, Leah and Hase, Johann v. and Weiland, Yanina and Kreja, Ludwika and Diebold, Joachim and Speicher, Michael R. and Cremer, Thomas}, year={2003}, month=sep, pages={809–820} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        31
      ]
    ],
    "date-time": "2025-08-31T10:14:52Z",
    "timestamp": 1756635292207
  },
  "reference-count": 38,
  "publisher": "Rockefeller University Press",
  "issue": "5",
  "content-domain": {
    "domain": [
      "rupress.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2003,
        9,
        1
      ]
    ]
  },
  "abstract": "<jats:p>A gene density\u2013related difference in the radial arrangement of chromosome territories (CTs) was previously described for human lymphocyte nuclei with gene-poor CT #18 located toward the nuclear periphery and gene-dense CT #19 in the nuclear interior (Croft, J.A., J.M. Bridger, S. Boyle, P. Perry, P. Teague, and W.A. Bickmore. 1999. J. Cell Biol. 145:1119\u20131131). Here, we analyzed the radial distribution of chromosome 18 and 19 chromatin in six normal cell types and in eight tumor cell lines, some of them with imbalances and rearrangements of the two chromosomes. Our findings demonstrate that a significant difference in the radial distribution of #18 and #19 chromatin is a common feature of higher order chromatin architecture in both normal and malignant cell types. However, in seven of eight tumor cell lines, the difference was less pronounced compared with normal cell nuclei due to a higher fraction of nuclei showing an inverted CT position, i.e., a CT #18 located more internally than a CT #19. This observation emphasizes a partial loss of radial chromatin order in tumor cell nuclei.</jats:p>",
  "DOI": "10.1083/jcb.200304096",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2003,
        9,
        2
      ]
    ],
    "date-time": "2003-09-02T20:37:03Z",
    "timestamp": 1062535023000
  },
  "page": "809-820",
  "update-policy": "http://dx.doi.org/10.1083/jcb.crossmarkpolicy",
  "source": "Crossref",
  "is-referenced-by-count": 208,
  "title": "Inheritance of gene density\u2013related higher order chromatin arrangements in normal and tumor cell nuclei",
  "prefix": "10.1083",
  "volume": "162",
  "author": [
    {
      "given": "Marion",
      "family": "Cremer",
      "sequence": "first",
      "affiliation": [
        {
          "name": "1Department of Biology II, Ludwig Maximilians University, 80333 Munich, Germany"
        },
        {
          "name": "3Institute of Human Genetics, Technical University and GSF, 81675 Munich, Germany"
        }
      ]
    },
    {
      "given": "Katrin",
      "family": "Ku\u0308pper",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "1Department of Biology II, Ludwig Maximilians University, 80333 Munich, Germany"
        }
      ]
    },
    {
      "given": "Babett",
      "family": "Wagler",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "1Department of Biology II, Ludwig Maximilians University, 80333 Munich, Germany"
        }
      ]
    },
    {
      "given": "Leah",
      "family": "Wizelman",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "1Department of Biology II, Ludwig Maximilians University, 80333 Munich, Germany"
        }
      ]
    },
    {
      "given": "Johann v.",
      "family": "Hase",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "2Kirchhoff-Institute of Physics, University of Heidelberg, 69120 Heidelberg, Germany"
        }
      ]
    },
    {
      "given": "Yanina",
      "family": "Weiland",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "3Institute of Human Genetics, Technical University and GSF, 81675 Munich, Germany"
        }
      ]
    },
    {
      "given": "Ludwika",
      "family": "Kreja",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "4Institute for Occupational, Social and Environmental Medicine, University of Ulm, 89070 Ulm, Germany"
        }
      ]
    },
    {
      "given": "Joachim",
      "family": "Diebold",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "5Institute of Pathology, University of Munich, 80337 Munich, Germany"
        }
      ]
    },
    {
      "given": "Michael R.",
      "family": "Speicher",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "3Institute of Human Genetics, Technical University and GSF, 81675 Munich, Germany"
        }
      ]
    },
    {
      "given": "Thomas",
      "family": "Cremer",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "1Department of Biology II, Ludwig Maximilians University, 80333 Munich, Germany"
        }
      ]
    }
  ],
  "member": "291",
  "published-online": {
    "date-parts": [
      [
        2003,
        9,
        2
      ]
    ]
  },
  "reference": [
    {
      "key": "2023072214283798500_BIB1",
      "doi-asserted-by": "crossref",
      "first-page": "2538",
      "DOI": "10.1073/pnas.041603298",
      "volume": "98",
      "year": "2001",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072214283798500_BIB2",
      "doi-asserted-by": "crossref",
      "first-page": "469",
      "DOI": "10.1093/carcin/21.3.469",
      "volume": "21",
      "year": "2000",
      "journal-title": "Carcinogenesis."
    },
    {
      "key": "2023072214283798500_BIB3",
      "doi-asserted-by": "crossref",
      "first-page": "1",
      "DOI": "10.1007/BF02616180",
      "volume": "5",
      "year": "1962",
      "journal-title": "Metrika"
    },
    {
      "key": "2023072214283798500_BIB4",
      "doi-asserted-by": "crossref",
      "first-page": "167",
      "DOI": "10.1242/jcs.77.1.167",
      "volume": "77",
      "year": "1985",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "2023072214283798500_BIB5",
      "doi-asserted-by": "crossref",
      "first-page": "211",
      "DOI": "10.1093/hmg/10.3.211",
      "volume": "10",
      "year": "2001",
      "journal-title": "Hum. Mol. Genet."
    },
    {
      "key": "2023072214283798500_BIB6",
      "doi-asserted-by": "crossref",
      "first-page": "149",
      "DOI": "10.1016/S0960-9822(00)00312-2",
      "volume": "10",
      "year": "2000",
      "journal-title": "Curr. Biol."
    },
    {
      "key": "2023072214283798500_BIB7",
      "doi-asserted-by": "crossref",
      "first-page": "300",
      "DOI": "10.1038/32688",
      "volume": "392",
      "year": "1998",
      "journal-title": "Nature."
    },
    {
      "key": "2023072214283798500_BIB8",
      "doi-asserted-by": "crossref",
      "first-page": "97",
      "DOI": "10.1016/S0165-0270(98)00066-1",
      "volume": "83",
      "year": "1998",
      "journal-title": "J. Neurosci. Methods."
    },
    {
      "key": "2023072214283798500_BIB9",
      "doi-asserted-by": "crossref",
      "first-page": "541",
      "DOI": "10.1023/A:1012495201697",
      "volume": "9",
      "year": "2001",
      "journal-title": "Chromosome Res."
    },
    {
      "key": "2023072214283798500_BIB10",
      "doi-asserted-by": "crossref",
      "first-page": "1119",
      "DOI": "10.1083/jcb.145.6.1119",
      "volume": "145",
      "year": "1999",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072214283798500_BIB11",
      "doi-asserted-by": "crossref",
      "first-page": "4037",
      "DOI": "10.1242/jcs.00087",
      "volume": "115",
      "year": "2002",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "2023072214283798500_BIB12",
      "doi-asserted-by": "crossref",
      "first-page": "590",
      "DOI": "10.1007/BF00202476",
      "volume": "90",
      "year": "1993",
      "journal-title": "Hum. Genet."
    },
    {
      "key": "2023072214283798500_BIB13",
      "doi-asserted-by": "crossref",
      "first-page": "41",
      "DOI": "10.1155/2000/502753",
      "volume": "20",
      "year": "2000",
      "journal-title": "Anal. Cell. Pathol."
    },
    {
      "key": "2023072214283798500_BIB14",
      "doi-asserted-by": "crossref",
      "first-page": "751",
      "DOI": "10.1016/S0092-8674(03)00189-2",
      "volume": "112",
      "year": "2003",
      "journal-title": "Cell."
    },
    {
      "key": "2023072214283798500_BIB15",
      "doi-asserted-by": "crossref",
      "first-page": "190",
      "DOI": "10.1016/0268-960X(93)90005-O",
      "volume": "7",
      "year": "1993",
      "journal-title": "Blood Rev."
    },
    {
      "key": "2023072214283798500_BIB16",
      "doi-asserted-by": "crossref",
      "first-page": "569",
      "DOI": "10.1023/A:1012447318535",
      "volume": "9",
      "year": "2001",
      "journal-title": "Chromosome Res."
    },
    {
      "key": "2023072214283798500_BIB17",
      "doi-asserted-by": "crossref",
      "first-page": "322",
      "DOI": "10.1016/0014-4827(88)90271-6",
      "volume": "179",
      "year": "1988",
      "journal-title": "Exp. Cell Res."
    },
    {
      "key": "2023072214283798500_BIB18",
      "doi-asserted-by": "crossref",
      "first-page": "8611",
      "DOI": "10.1073/pnas.89.18.8611",
      "volume": "89",
      "year": "1992",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072214283798500_BIB19",
      "doi-asserted-by": "crossref",
      "first-page": "825",
      "DOI": "10.1002/eji.1830111015",
      "volume": "11",
      "year": "1981",
      "journal-title": "Eur. J. Immunol."
    },
    {
      "key": "2023072214283798500_BIB20",
      "doi-asserted-by": "crossref",
      "first-page": "489",
      "DOI": "10.1002/ijc.10845",
      "volume": "103",
      "year": "2003",
      "journal-title": "Int. J. Cancer."
    },
    {
      "key": "2023072214283798500_BIB21",
      "doi-asserted-by": "crossref",
      "first-page": "377",
      "DOI": "10.1016/S0960-9822(98)70156-3",
      "volume": "8",
      "year": "1998",
      "journal-title": "Curr. Biol."
    },
    {
      "key": "2023072214283798500_BIB22",
      "doi-asserted-by": "crossref",
      "first-page": "351",
      "DOI": "10.1002/1096-9896(2000)9999:9999<::AID-PATH708>3.0.CO;2-I",
      "volume": "192",
      "year": "2000",
      "journal-title": "J. Pathol."
    },
    {
      "key": "2023072214283798500_BIB23",
      "doi-asserted-by": "crossref",
      "first-page": "321",
      "DOI": "10.1007/s00412-002-0210-8",
      "volume": "111",
      "year": "2002",
      "journal-title": "Chromosoma."
    },
    {
      "key": "2023072214283798500_BIB24",
      "doi-asserted-by": "crossref",
      "first-page": "159",
      "DOI": "10.1002/gcc.10160",
      "volume": "36",
      "year": "2003",
      "journal-title": "Genes Chromosomes Cancer."
    },
    {
      "key": "2023072214283798500_BIB25",
      "doi-asserted-by": "crossref",
      "first-page": "1",
      "DOI": "10.1182/blood.V87.1.1.1",
      "volume": "87",
      "year": "1996",
      "journal-title": "Blood."
    },
    {
      "key": "2023072214283798500_BIB26",
      "doi-asserted-by": "crossref",
      "first-page": "11",
      "DOI": "10.1093/annonc/13.S1.11",
      "volume": "13",
      "year": "2002",
      "journal-title": "Ann. Oncol."
    },
    {
      "key": "2023072214283798500_BIB27",
      "doi-asserted-by": "crossref",
      "first-page": "286",
      "DOI": "10.1016/S0955-0674(02)00335-6",
      "volume": "14",
      "year": "2002",
      "journal-title": "Curr. Opin. Cell Biol."
    },
    {
      "key": "2023072214283798500_BIB28",
      "first-page": "4562",
      "volume": "36",
      "year": "1976",
      "journal-title": "Cancer Res."
    },
    {
      "key": "2023072214283798500_BIB29",
      "doi-asserted-by": "crossref",
      "first-page": "153",
      "DOI": "10.1111/j.1432-0436.1983.tb01316.x",
      "volume": "24",
      "year": "1983",
      "journal-title": "Differentiation."
    },
    {
      "key": "2023072214283798500_BIB30",
      "doi-asserted-by": "crossref",
      "first-page": "1692",
      "DOI": "10.1016/S0960-9822(02)01166-1",
      "volume": "12",
      "year": "2002",
      "journal-title": "Curr. Biol."
    },
    {
      "key": "2023072214283798500_BIB31",
      "doi-asserted-by": "crossref",
      "first-page": "362",
      "DOI": "10.2144/99272rr04",
      "volume": "27",
      "year": "1999",
      "journal-title": "Biotechniques."
    },
    {
      "key": "2023072214283798500_BIB32",
      "doi-asserted-by": "crossref",
      "first-page": "621",
      "DOI": "10.1002/ijc.2910190505",
      "volume": "19",
      "year": "1977",
      "journal-title": "Int. J. Cancer."
    },
    {
      "key": "2023072214283798500_BIB33",
      "doi-asserted-by": "crossref",
      "first-page": "719",
      "DOI": "10.1177/002215549904700601",
      "volume": "47",
      "year": "1999",
      "journal-title": "J. Histochem. Cytochem."
    },
    {
      "key": "2023072214283798500_BIB34",
      "doi-asserted-by": "crossref",
      "first-page": "487",
      "DOI": "10.1023/A:1009267605580",
      "volume": "8",
      "year": "2000",
      "journal-title": "Chromosome Res."
    },
    {
      "key": "2023072214283798500_BIB35"
    },
    {
      "key": "2023072214283798500_BIB36",
      "doi-asserted-by": "crossref",
      "first-page": "37",
      "DOI": "10.1016/S0027-5107(02)00077-5",
      "volume": "504",
      "year": "2002",
      "journal-title": "Mutat. Res."
    },
    {
      "key": "2023072214283798500_BIB37",
      "doi-asserted-by": "crossref",
      "first-page": "4424",
      "DOI": "10.1073/pnas.072618599",
      "volume": "99",
      "year": "2002",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072214283798500_BIB38",
      "doi-asserted-by": "crossref",
      "first-page": "485",
      "DOI": "10.1023/A:1025016828544",
      "volume": "11",
      "year": "2003",
      "journal-title": "Chromosome Res."
    }
  ],
  "container-title": "The Journal of Cell Biology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://rupress.org/jcb/article-pdf/162/5/809/1524692/jcb1625809.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://rupress.org/jcb/article-pdf/162/5/809/1524692/jcb1625809.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        7,
        22
      ]
    ],
    "date-time": "2023-07-22T14:29:24Z",
    "timestamp": 1690036164000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://rupress.org/jcb/article/162/5/809/33623/Inheritance-of-gene-density-related-higher-order"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2003,
        9,
        1
      ]
    ]
  },
  "references-count": 38,
  "journal-issue": {
    "issue": "5",
    "published-print": {
      "date-parts": [
        [
          2003,
          9,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1083/jcb.200304096",
  "relation": {
    "has-review": [
      {
        "id-type": "doi",
        "id": "10.3410/f.1016710.201282",
        "asserted-by": "object"
      }
    ]
  },
  "ISSN": [
    "1540-8140",
    "0021-9525"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        2003,
        9,
        1
      ]
    ]
  }
}