DOI: 10.1083/jcb.200304046. The <i>Drosophila</i> nucleoporin DNup88 localizes DNup214 and CRM1 on the nuclear envelope and attenuates NES-mediated nuclear export

The Drosophila nucleoporin DNup88 localizes DNup214 and CRM1 on the nuclear envelope and attenuates NES-mediated nuclear export

10.1083/jcb.200304046

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Many cellular responses rely on the control of nucleocytoplasmic transport of transcriptional regulators. The Drosophila nucleoporin Nup88 is selectively required for nuclear accumulation of Rel proteins and full activation of the innate immune response. Here, we investigate the mechanisms underlying its role in nucleocytoplasmic transport. Nuclear import of an nuclear localization signal-enhanced green fluorescent protein (NLS-EGFP) reporter is not affected in DNup88 (members only; mbo) mutants, whereas the level of CRM1-dependent EGFP-nuclear export signal (EGFP-NES) export is increased. We show that the nuclear accumulation of the Drosophila Rel protein Dorsal requires CRM1. DNup88 binds to DNup214 and DCRM1 in vitro, and both proteins become mislocalized from the nuclear rim into the nucleus of mbo mutants. Overexpression of DNup88 is sufficient to relocalize DNup214 and CRM1 on the nuclear envelope and revert the mutant phenotypes. We propose that a major function of DNup88 is to anchor DNup214 and CRM1 on the nuclear envelope and thereby attenuate NES-mediated nuclear export.

Bibliography

Roth, P., Xylourgidis, N., Sabri, N., Uv, A., Fornerod, M., & Samakovlis, C. (2003). The Drosophila nucleoporin DNup88 localizes DNup214 and CRM1 on the nuclear envelope and attenuates NES-mediated nuclear export. The Journal of Cell Biology, 163(4), 701â706.

Authors 6

Peggy Roth (first)
Nikos Xylourgidis (additional)
Nafiseh Sabri (additional)
Anne Uv (additional)
Maarten Fornerod (additional)
Christos Samakovlis (additional)

References 35 Referenced 75

{'key': '2023072214465174800_BIB1'}
10.1091/mbc.9.12.3475 / Mol. Biol. Cell (1998)
10.1016/S0925-4773(96)00607-7 / Mech. Dev. (1996)
10.1074/jbc.M006179200 / J. Biol. Chem. (2000)
10.1093/genetics/155.4.1799 / Genetics. (2000)
10.1083/jcb.123.6.1333 / J. Cell Biol. (1993)
10.1083/jcb.200206106 / J. Cell Biol. (2002)
10.1074/jbc.274.23.16279 / J. Biol. Chem. (1999)
10.1016/S0092-8674(00)80371-2 / Cell. (1997)
10.1093/emboj/16.4.807 / EMBO J. (1997)
{'key': '2023072214465174800_BIB11', 'first-page': '3559', 'volume': '14', 'year': '1994', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. (1994)
10.1038/377246a0 / Nature. (1995)
10.1083/jcb.138.1.65 / J. Cell Biol. (1997)
10.1128/MCB.20.23.8996-9008.2000 / Mol. Cell. Biol. (2000)
{'key': '2023072214465174800_BIB15', 'first-page': '1768', 'volume': '7', 'year': '2001', 'journal-title': 'RNA'} / RNA (2001)
10.1101/gad.6.4.619 / Genes Dev. (1992)
10.1016/0092-8674(84)90457-4 / Cell. (1984)
10.1146/annurev.genet.35.102401.090720 / Annu. Rev. Genet. (2001)
10.1073/pnas.91.4.1519 / Proc. Natl. Acad. Sci. USA. (1994)
10.1006/excr.1998.4136 / Exp. Cell Res. (1998)
10.1016/S1534-5807(02)00134-X / Dev. Cell. (2002)
10.1093/genetics/156.4.1889 / Genetics. (2000)
10.1016/S0092-8674(00)81862-0 / Cell. (1997)
10.1146/annurev.biochem.67.1.265 / Annu. Rev. Biochem. (1998)
10.1083/jcb.140.3.499 / J. Cell Biol. (1998)
10.1126/science.283.5408.1742 / Science. (1999)
{'key': '2023072214465174800_BIB27'}
10.1016/0092-8674(89)90772-1 / Cell. (1989)
{'key': '2023072214465174800_BIB29'}
10.1242/jcs.108.9.3137 / J. Cell Sci. (1995)
{'key': '2023072214465174800_BIB31', 'first-page': '4020', 'volume': '14', 'year': '1994', 'journal-title': 'Mol. Cell. Biol.'} / Mol. Cell. Biol. (1994)
10.1101/gad.14.15.1945 / Genes Dev. (2000)
10.1016/S0092-8674(03)00082-5 / Cell. (2003)
10.1016/0092-8674(95)90435-2 / Cell. (1995)
{'key': '2023072214465174800_BIB35', 'first-page': '1781', 'volume': '7', 'year': '2001', 'journal-title': 'RNA.'} / RNA. (2001)

Dates

Type	When
Created	21 years, 9 months ago (Nov. 24, 2003, 5:12 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 10:47 a.m.)
Indexed	2 months, 1 week ago (June 21, 2025, 8:24 a.m.)
Issued	21 years, 9 months ago (Nov. 24, 2003)
Published	21 years, 9 months ago (Nov. 24, 2003)
Published Online	21 years, 9 months ago (Nov. 24, 2003)
Published Print	21 years, 9 months ago (Nov. 24, 2003)

Funders 0

None

BibTeX

@article{Roth_2003, title={The Drosophila nucleoporin DNup88 localizes DNup214 and CRM1 on the nuclear envelope and attenuates NES-mediated nuclear export}, volume={163}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200304046}, DOI={10.1083/jcb.200304046}, number={4}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Roth, Peggy and Xylourgidis, Nikos and Sabri, Nafiseh and Uv, Anne and Fornerod, Maarten and Samakovlis, Christos}, year={2003}, month=nov, pages={701–706} }

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