DOI: 10.1083/jcb.200303192. Gliotactin, a novel marker of tricellular junctions, is necessary for septate junction development in <i>Drosophila </i>

Gliotactin, a novel marker of tricellular junctions, is necessary for septate junction development in Drosophila

10.1083/jcb.200303192

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Septate junctions (SJs), similar to tight junctions, function as transepithelial permeability barriers. Gliotactin (Gli) is a cholinesterase-like molecule that is necessary for blood–nerve barrier integrity, and may, therefore, contribute to SJ development or function. To address this hypothesis, we analyzed Gli expression and the Gli mutant phenotype in Drosophila epithelia. In Gli mutants, localization of SJ markers neurexin-IV, discs large, and coracle are disrupted. Furthermore, SJ barrier function is lost as determined by dye permeability assays. These data suggest that Gli is necessary for SJ formation. Surprisingly, Gli distribution only colocalizes with other SJ markers at tricellular junctions, suggesting that Gli has a unique function in SJ development. Ultrastructural analysis of Gli mutants supports this notion. In contrast to other SJ mutants in which septa are missing, septa are present in Gli mutants, but the junction has an immature morphology. We propose a model, whereby Gli acts at tricellular junctions to bind, anchor, or compact SJ strands apically during SJ development.

Bibliography

Schulte, J., Tepass, U., & Auld, V. J. (2003). Gliotactin, a novel marker of tricellular junctions, is necessary for septate junction development in Drosophila . The Journal of Cell Biology, 161(5), 991â1000.

Authors 3

Joost Schulte (first)
Ulrich Tepass (additional)
Vanessa J. Auld (additional)

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Dates

Type	When
Created	22 years, 2 months ago (June 3, 2003, 9:27 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 11:41 a.m.)
Indexed	1 week, 2 days ago (Aug. 23, 2025, 12:59 a.m.)
Issued	22 years, 2 months ago (June 2, 2003)
Published	22 years, 2 months ago (June 2, 2003)
Published Online	22 years, 2 months ago (June 2, 2003)
Published Print	22 years, 2 months ago (June 9, 2003)

Funders 0

None

BibTeX

@article{Schulte_2003, title={Gliotactin, a novel marker of tricellular junctions, is necessary for septate junction development in Drosophila }, volume={161}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200303192}, DOI={10.1083/jcb.200303192}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Schulte, Joost and Tepass, Ulrich and Auld, Vanessa J.}, year={2003}, month=jun, pages={991–1000} }

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