DOI: 10.1083/jcb.200303174. Distinct roles of Gαi and Gβ13F subunits of the heterotrimeric G protein complex in the mediation of <i>Drosophila</i> neuroblast asymmetric divisions

Distinct roles of Gαi and Gβ13F subunits of the heterotrimeric G protein complex in the mediation of Drosophila neuroblast asymmetric divisions

10.1083/jcb.200303174

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The asymmetric division of Drosophila neuroblasts involves the basal localization of cell fate determinants and the generation of an asymmetric, apicobasally oriented mitotic spindle that leads to the formation of two daughter cells of unequal size. These features are thought to be controlled by an apically localized protein complex comprising of two signaling pathways: Bazooka/Drosophila atypical PKC/Inscuteable/DmPar6 and Partner of inscuteable (Pins)/Gαi; in addition, Gβ13F is also required. However, the role of Gαi and the hierarchical relationship between the G protein subunits and apical components are not well defined. Here we describe the isolation of Gαi mutants and show that Gαi and Gβ13F play distinct roles. Gαi is required for Pins to localize to the cortex, and the effects of loss of Gαi or pins are highly similar, supporting the idea that Pins/Gαi act together to mediate various aspects of neuroblast asymmetric division. In contrast, Gβ13F appears to regulate the asymmetric localization/stability of all apical components, and Gβ13F loss of function exhibits phenotypes resembling those seen when both apical pathways have been compromised, suggesting that it acts upstream of the apical pathways. Importantly, our results have also revealed a novel aspect of apical complex function, that is, the two apical pathways act redundantly to suppress the formation of basal astral microtubules in neuroblasts.

Bibliography

Yu, F., Cai, Y., Kaushik, R., Yang, X., & Chia, W. (2003). Distinct roles of GÎ±i and GÎ²13F subunits of the heterotrimeric G protein complex in the mediation of Drosophila neuroblast asymmetric divisions. The Journal of Cell Biology, 162(4), 623â633.

Authors 5

Fengwei Yu (first)
Yu Cai (additional)
Rachna Kaushik (additional)
Xiaohang Yang (additional)
William Chia (additional)

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Dates

Type	When
Created	22 years ago (Aug. 18, 2003, 2:05 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 10:31 a.m.)
Indexed	5 months ago (March 31, 2025, 4:43 a.m.)
Issued	22 years ago (Aug. 18, 2003)
Published	22 years ago (Aug. 18, 2003)
Published Online	22 years ago (Aug. 18, 2003)
Published Print	22 years ago (Aug. 18, 2003)

Funders 0

None

BibTeX

@article{Yu_2003, title={Distinct roles of Gαi and Gβ13F subunits of the heterotrimeric G protein complex in the mediation of Drosophila neuroblast asymmetric divisions}, volume={162}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200303174}, DOI={10.1083/jcb.200303174}, number={4}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Yu, Fengwei and Cai, Yu and Kaushik, Rachna and Yang, Xiaohang and Chia, William}, year={2003}, month=aug, pages={623–633} }

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