DOI: 10.1083/jcb.200303088. Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane

Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane

10.1083/jcb.200303088

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

It was known that the uptake of tryptophan is reduced in the yeast erg6 mutant, which is defective in a late step of ergosterol biosynthesis. Here, we show that this is because the high affinity tryptophan permease Tat2p is not targeted to the plasma membrane. In wild-type cells, the plasma membrane localization of Tat2p is regulated by the external tryptophan concentration. Tat2p is transported from the Golgi apparatus to the vacuole at high tryptophan, and to the plasma membrane at low tryptophan. However, in the erg6 mutant, Tat2p is missorted to the vacuole at low tryptophan. The plasma membrane targeting of Tat2p is dependent on detergent-insoluble membrane domains, suggesting that sterol affects the sorting through the organization of lipid rafts. The erg6 mutation also caused missorting to the multivesicular body pathway in late endosomes. Thus, sterol composition is crucial for protein sorting late in the secretory pathway. Tat2p is subject to polyubiquitination, which acts as a vacuolar-targeting signal, and the inhibition of this process suppresses the Tat2p sorting defects of the erg6 mutant. The sorting mechanisms of Tat2p that depend on both sterol and ubiquitin will be discussed.

Bibliography

Umebayashi, K., & Nakano, A. (2003). Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. The Journal of Cell Biology, 161(6), 1117â1131.

Authors 2

Kyohei Umebayashi (first)
Akihiko Nakano (additional)

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Dates

Type	When
Created	22 years, 2 months ago (June 16, 2003, 8:35 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 10:30 a.m.)
Indexed	3 months ago (June 5, 2025, 2:27 a.m.)
Issued	22 years, 2 months ago (June 16, 2003)
Published	22 years, 2 months ago (June 16, 2003)
Published Online	22 years, 2 months ago (June 16, 2003)
Published Print	22 years, 2 months ago (June 23, 2003)

Funders 0

None

BibTeX

@article{Umebayashi_2003, title={Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane}, volume={161}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200303088}, DOI={10.1083/jcb.200303088}, number={6}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Umebayashi, Kyohei and Nakano, Akihiko}, year={2003}, month=jun, pages={1117–1131} }

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