DOI: 10.1083/jcb.200303023. Molecular requirements for actin-based lamella formation in <i>Drosophila</i> S2 cells

Molecular requirements for actin-based lamella formation in Drosophila S2 cells

10.1083/jcb.200303023

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Cell migration occurs through the protrusion of the actin-enriched lamella. Here, we investigated the effects of RNAi depletion of ∼90 proteins implicated in actin function on lamella formation in Drosophila S2 cells. Similar to in vitro reconstitution studies of actin-based Listeria movement, we find that lamellae formation requires a relatively small set of proteins that participate in actin nucleation (Arp2/3 and SCAR), barbed end capping (capping protein), filament depolymerization (cofilin and Aip1), and actin monomer binding (profilin and cyclase-associated protein). Lamellae are initiated by parallel and partially redundant signaling pathways involving Rac GTPases and the adaptor protein Nck, which stimulate SCAR, an Arp2/3 activator. We also show that RNAi of three proteins (kette, Abi, and Sra-1) known to copurify with and inhibit SCAR in vitro leads to SCAR degradation, revealing a novel function of this protein complex in SCAR stability. Our results have identified an essential set of proteins involved in actin dynamics during lamella formation in Drosophila S2 cells.

Bibliography

Rogers, S. L., Wiedemann, U., Stuurman, N., & Vale, R. D. (2003). Molecular requirements for actin-based lamella formation in Drosophila S2 cells. The Journal of Cell Biology, 162(6), 1079â1088.

Authors 4

Stephen L. Rogers (first)
Ursula Wiedemann (additional)
Nico Stuurman (additional)
Ronald D. Vale (additional)

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Dates

Type	When
Created	21 years, 11 months ago (Sept. 15, 2003, 3:27 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 10:35 a.m.)
Indexed	1 month, 1 week ago (July 14, 2025, 11:34 p.m.)
Issued	21 years, 11 months ago (Sept. 15, 2003)
Published	21 years, 11 months ago (Sept. 15, 2003)
Published Online	21 years, 11 months ago (Sept. 15, 2003)
Published Print	21 years, 11 months ago (Sept. 15, 2003)

Funders 0

None

BibTeX

@article{Rogers_2003, title={Molecular requirements for actin-based lamella formation in Drosophila S2 cells}, volume={162}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200303023}, DOI={10.1083/jcb.200303023}, number={6}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Rogers, Stephen L. and Wiedemann, Ursula and Stuurman, Nico and Vale, Ronald D.}, year={2003}, month=sep, pages={1079–1088} }

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