DOI: 10.1083/jcb.200212037. Macrophage podosomes assemble at the leading lamella by growth and fragmentation

Macrophage podosomes assemble at the leading lamella by growth and fragmentation

10.1083/jcb.200212037

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Podosomes are actin- and fimbrin-containing adhesions at the leading edge of macrophages. In cells transfected with β-actin–ECFP and L-fimbrin–EYFP, quantitative four-dimensional microscopy of podosome assembly shows that new adhesions arise at the cell periphery by one of two mechanisms; de novo podosome assembly, or fission of a precursor podosome into daughter podosomes. The large podosome cluster precursor also appears to be an adhesion structure; it contains actin, fimbrin, integrin, and is in close apposition to the substratum. Microtubule inhibitors paclitaxel and demecolcine inhibit the turnover and polarized formation of podosomes, but not the turnover rate of actin in these structures. Because daughter podosomes and podosome cluster precursors are preferentially located at the leading edge, they may play a critical role in continually generating new sites of cell adhesion.

Bibliography

Evans, J. G., Correia, I., Krasavina, O., Watson, N., & Matsudaira, P. (2003). Macrophage podosomes assemble at the leading lamella by growth and fragmentation. The Journal of Cell Biology, 161(4), 697â705.

Authors 5

James G. Evans (first)
Ivan Correia (additional)
Olga Krasavina (additional)
Nicki Watson (additional)
Paul Matsudaira (additional)

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Dates

Type	When
Created	22 years, 3 months ago (May 19, 2003, 9:48 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 10:23 a.m.)
Indexed	3 months ago (May 21, 2025, 1:26 p.m.)
Issued	22 years, 3 months ago (May 19, 2003)
Published	22 years, 3 months ago (May 19, 2003)
Published Online	22 years, 3 months ago (May 19, 2003)
Published Print	22 years, 2 months ago (May 26, 2003)

Funders 0

None

BibTeX

@article{Evans_2003, title={Macrophage podosomes assemble at the leading lamella by growth and fragmentation}, volume={161}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200212037}, DOI={10.1083/jcb.200212037}, number={4}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Evans, James G. and Correia, Ivan and Krasavina, Olga and Watson, Nicki and Matsudaira, Paul}, year={2003}, month=may, pages={697–705} }

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