DOI: 10.1083/jcb.200211015. The conformational state of Tes regulates its zyxin-dependent recruitment to focal adhesions

The conformational state of Tes regulates its zyxin-dependent recruitment to focal adhesions

10.1083/jcb.200211015

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The function of the human Tes protein, which has extensive similarity to zyxin in both sequence and domain organization, is currently unknown. We now show that Tes is a component of focal adhesions that, when expressed, negatively regulates proliferation of T47D breast carcinoma cells. Coimmunoprecipitations demonstrate that in vivo Tes is complexed with actin, Mena, and vasodilator-stimulated phosphoprotein (VASP). Interestingly, the isolated NH2-terminal half of Tes pulls out α-actinin and paxillin from cell extracts in addition to actin. The COOH-terminal half recruits zyxin as well as Mena and VASP from cell extracts. These differences suggest that the ability of Tes to associate with α-actinin, paxillin, and zyxin is dependent on the conformational state of the molecule. Consistent with this hypothesis, we demonstrate that the two halves of Tes interact with each other in vitro and in vivo. Using fibroblasts lacking Mena and VASP, we show that these proteins are not required to recruit Tes to focal adhesions. However, using RNAi ablation, we demonstrate that zyxin is required to recruit Tes, as well as Mena and VASP, but not vinculin or paxillin, to focal adhesions.

Bibliography

Garvalov, B. K., Higgins, T. E., Sutherland, J. D., Zettl, M., Scaplehorn, N., KoÌcher, T., Piddini, E., Griffiths, G., & Way, M. (2003). The conformational state of Tes regulates its zyxin-dependent recruitment to focal adhesions. The Journal of Cell Biology, 161(1), 33â39.

Authors 9

Boyan K. Garvalov (first)
Theresa E. Higgins (additional)
James D. Sutherland (additional)
Markus Zettl (additional)
Niki Scaplehorn (additional)
Thomas Köcher (additional)
Eugenia Piddini (additional)
Gareth Griffiths (additional)
Michael Way (additional)

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{'key': '2023072214151569200_BIB6'}
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Dates

Type	When
Created	22 years, 4 months ago (April 14, 2003, 2:18 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 10:16 a.m.)
Indexed	3 months ago (June 5, 2025, 4:04 a.m.)
Issued	22 years, 4 months ago (April 14, 2003)
Published	22 years, 4 months ago (April 14, 2003)
Published Online	22 years, 4 months ago (April 14, 2003)
Published Print	22 years, 4 months ago (April 14, 2003)

Funders 0

None

BibTeX

@article{Garvalov_2003, title={The conformational state of Tes regulates its zyxin-dependent recruitment to focal adhesions}, volume={161}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200211015}, DOI={10.1083/jcb.200211015}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Garvalov, Boyan K. and Higgins, Theresa E. and Sutherland, James D. and Zettl, Markus and Scaplehorn, Niki and Köcher, Thomas and Piddini, Eugenia and Griffiths, Gareth and Way, Michael}, year={2003}, month=apr, pages={33–39} }

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