DOI: 10.1083/jcb.200208092. The small molecule Hesperadin reveals a role for Aurora B in correcting kinetochore–microtubule attachment and in maintaining the spindle assembly checkpoint

The small molecule Hesperadin reveals a role for Aurora B in correcting kinetochore–microtubule attachment and in maintaining the spindle assembly checkpoint

10.1083/jcb.200208092

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The proper segregation of sister chromatids in mitosis depends on bipolar attachment of all chromosomes to the mitotic spindle. We have identified the small molecule Hesperadin as an inhibitor of chromosome alignment and segregation. Our data imply that Hesperadin causes this phenotype by inhibiting the function of the mitotic kinase Aurora B. Mammalian cells treated with Hesperadin enter anaphase in the presence of numerous monooriented chromosomes, many of which may have both sister kinetochores attached to one spindle pole (syntelic attachment). Hesperadin also causes cells arrested by taxol or monastrol to enter anaphase within &lt;1 h, whereas cells in nocodazole stay arrested for 3–5 h. Together, our data suggest that Aurora B is required to generate unattached kinetochores on monooriented chromosomes, which in turn could promote bipolar attachment as well as maintain checkpoint signaling.

Bibliography

Hauf, S., Cole, R. W., LaTerra, S., Zimmer, C., Schnapp, G., Walter, R., Heckel, A., van Meel, J., Rieder, C. L., & Peters, J.-M. (2003). The small molecule Hesperadin reveals a role for Aurora B in correcting kinetochoreâmicrotubule attachment and in maintaining the spindle assembly checkpoint. The Journal of Cell Biology, 161(2), 281â294.

Authors 10

Silke Hauf (first)
Richard W. Cole (additional)
Sabrina LaTerra (additional)
Christine Zimmer (additional)
Gisela Schnapp (additional)
Rainer Walter (additional)
Armin Heckel (additional)
Jacques van Meel (additional)
Conly L. Rieder (additional)
Jan-Michael Peters (additional)

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Dates

Type	When
Created	22 years, 4 months ago (April 28, 2003, 3:44 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 10:25 a.m.)
Indexed	3 weeks, 3 days ago (Aug. 5, 2025, 9:04 a.m.)
Issued	22 years, 4 months ago (April 21, 2003)
Published	22 years, 4 months ago (April 21, 2003)
Published Online	22 years, 4 months ago (April 21, 2003)
Published Print	22 years, 4 months ago (April 28, 2003)

Funders 0

None

BibTeX

@article{Hauf_2003, title={The small molecule Hesperadin reveals a role for Aurora B in correcting kinetochore–microtubule attachment and in maintaining the spindle assembly checkpoint}, volume={161}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200208092}, DOI={10.1083/jcb.200208092}, number={2}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Hauf, Silke and Cole, Richard W. and LaTerra, Sabrina and Zimmer, Christine and Schnapp, Gisela and Walter, Rainer and Heckel, Armin and van Meel, Jacques and Rieder, Conly L. and Peters, Jan-Michael}, year={2003}, month=apr, pages={281–294} }

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