DOI: 10.1083/jcb.200208074. Concentrative sorting of secretory cargo proteins into COPII-coated vesicles

Concentrative sorting of secretory cargo proteins into COPII-coated vesicles

10.1083/jcb.200208074

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Here, we show that efficient transport of membrane and secretory proteins from the ER of Saccharomyces cerevisiae requires concentrative and signal-mediated sorting. Three independent markers of bulk flow transport out of the ER indicate that in the absence of an ER export signal, molecules are inefficiently captured into coat protein complex II (COPII)-coated vesicles. A soluble secretory protein, glycosylated pro–α-factor (gpαf), was enriched ∼20 fold in these vesicles relative to bulk flow markers. In the absence of Erv29p, a membrane protein that facilitates gpαf transport (Belden and Barlowe, 2001), gpαf is packaged into COPII vesicles as inefficiently as soluble bulk flow markers. We also found that a plasma membrane protein, the general amino acid permease (Gap1p), is enriched approximately threefold in COPII vesicles relative to membrane phospholipids. Mutation of a diacidic sequence present in the COOH-terminal cytosolic domain of Gap1p eliminated concentrative sorting of this protein.

Bibliography

Malkus, P., Jiang, F., & Schekman, R. (2002). Concentrative sorting of secretory cargo proteins into COPII-coated vesicles. The Journal of Cell Biology, 159(6), 915â921.

Authors 3

Per Malkus (first)
Feng Jiang (additional)
Randy Schekman (additional)

References 28 Referenced 106

{'key': '2023072213555872700_BIB1'}
10.1016/0092-8674(88)90196-1 / Cell. (1988)
10.1016/0092-8674(94)90359-X / Cell. (1994)
10.1126/science.1065224 / Science. (2001)
10.1038/nature01040 / Nature. (2002)
10.1126/science.286.5446.1882 / Science. (1999)
10.1016/S0021-9258(18)77399-7 / J. Biol. Chem. (1990)
10.1091/mbc.10.11.3549 / Mol. Biol. Cell. (1999)
10.1016/S0962-8924(00)88926-6 / Trends Cell Biol. (1995)
10.1046/j.1365-2958.1997.3771735.x / Mol. Microbiol. (1997)
10.1038/34438 / Nature. (1998)
10.1083/jcb.135.3.585 / J. Cell Biol. (1996)
10.1073/pnas.191359398 / Proc. Natl. Acad. Sci. USA. (2001)
10.1126/science.291.5502.316 / Science. (2001)
10.1016/S0092-8674(00)80608-X / Cell. (1999)
10.1073/pnas.241522198 / Proc. Natl. Acad. Sci. USA. (2001)
10.1126/science.277.5325.556 / Science. (1997)
10.1073/pnas.89.15.7227 / Proc. Natl. Acad. Sci. USA. (1992)
10.1083/jcb.135.4.895 / J. Cell Biol. (1996)
10.1126/science.271.5255.1526 / Science. (1996)
10.1007/BF00340712 / Curr. Genet. (1989)
10.1091/mbc.11.1.13 / Mol. Biol. Cell. (2000)
10.1016/S0076-6879(02)51852-8 / Methods Enzymol. (2002)
10.1073/pnas.250472397 / Proc. Natl. Acad. Sci. USA. (2000)
10.1093/emboj/20.23.6742 / EMBO J. (2001)
10.1016/0092-8674(87)90224-8 / Cell. (1987)
10.1016/0076-6879(92)19015-X / Methods Enzymol. (1992)
10.1074/jbc.270.51.30567 / J. Biol. Chem. (1995)

Dates

Type	When
Created	22 years, 8 months ago (Dec. 23, 2002, 2:43 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 9:56 a.m.)
Indexed	4 weeks, 2 days ago (Aug. 6, 2025, 9:48 a.m.)
Issued	22 years, 8 months ago (Dec. 23, 2002)
Published	22 years, 8 months ago (Dec. 23, 2002)
Published Online	22 years, 8 months ago (Dec. 23, 2002)
Published Print	22 years, 8 months ago (Dec. 23, 2002)

Funders 0

None

BibTeX

@article{Malkus_2002, title={Concentrative sorting of secretory cargo proteins into COPII-coated vesicles}, volume={159}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200208074}, DOI={10.1083/jcb.200208074}, number={6}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Malkus, Per and Jiang, Feng and Schekman, Randy}, year={2002}, month=dec, pages={915–921} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        6
      ]
    ],
    "date-time": "2025-08-06T13:48:50Z",
    "timestamp": 1754488130896
  },
  "reference-count": 28,
  "publisher": "Rockefeller University Press",
  "issue": "6",
  "content-domain": {
    "domain": [
      "rupress.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2002,
        12,
        23
      ]
    ]
  },
  "abstract": "<jats:p>Here, we show that efficient transport of membrane and secretory proteins from the ER of Saccharomyces cerevisiae requires concentrative and signal-mediated sorting. Three independent markers of bulk flow transport out of the ER indicate that in the absence of an ER export signal, molecules are inefficiently captured into coat protein complex II (COPII)-coated vesicles. A soluble secretory protein, glycosylated pro\u2013\u03b1-factor (gp\u03b1f), was enriched \u223c20 fold in these vesicles relative to bulk flow markers. In the absence of Erv29p, a membrane protein that facilitates gp\u03b1f transport (Belden and Barlowe, 2001), gp\u03b1f is packaged into COPII vesicles as inefficiently as soluble bulk flow markers. We also found that a plasma membrane protein, the general amino acid permease (Gap1p), is enriched approximately threefold in COPII vesicles relative to membrane phospholipids. Mutation of a diacidic sequence present in the COOH-terminal cytosolic domain of Gap1p eliminated concentrative sorting of this protein.</jats:p>",
  "DOI": "10.1083/jcb.200208074",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        12,
        23
      ]
    ],
    "date-time": "2002-12-23T19:43:29Z",
    "timestamp": 1040672609000
  },
  "page": "915-921",
  "update-policy": "http://dx.doi.org/10.1083/jcb.crossmarkpolicy",
  "source": "Crossref",
  "is-referenced-by-count": 106,
  "title": "Concentrative sorting of secretory cargo proteins into COPII-coated vesicles",
  "prefix": "10.1083",
  "volume": "159",
  "author": [
    {
      "given": "Per",
      "family": "Malkus",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720"
        }
      ]
    },
    {
      "given": "Feng",
      "family": "Jiang",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720"
        }
      ]
    },
    {
      "given": "Randy",
      "family": "Schekman",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720"
        }
      ]
    }
  ],
  "member": "291",
  "published-online": {
    "date-parts": [
      [
        2002,
        12,
        23
      ]
    ]
  },
  "reference": [
    {
      "key": "2023072213555872700_BIB1"
    },
    {
      "key": "2023072213555872700_BIB2",
      "doi-asserted-by": "crossref",
      "first-page": "335",
      "DOI": "10.1016/0092-8674(88)90196-1",
      "volume": "54",
      "year": "1988",
      "journal-title": "Cell."
    },
    {
      "key": "2023072213555872700_BIB3",
      "doi-asserted-by": "crossref",
      "first-page": "841",
      "DOI": "10.1016/0092-8674(94)90359-X",
      "volume": "76",
      "year": "1994",
      "journal-title": "Cell."
    },
    {
      "key": "2023072213555872700_BIB4",
      "doi-asserted-by": "crossref",
      "first-page": "1528",
      "DOI": "10.1126/science.1065224",
      "volume": "294",
      "year": "2001",
      "journal-title": "Science."
    },
    {
      "key": "2023072213555872700_BIB5",
      "doi-asserted-by": "crossref",
      "first-page": "271",
      "DOI": "10.1038/nature01040",
      "volume": "419",
      "year": "2002",
      "journal-title": "Nature."
    },
    {
      "key": "2023072213555872700_BIB6",
      "doi-asserted-by": "crossref",
      "first-page": "1882",
      "DOI": "10.1126/science.286.5446.1882",
      "volume": "286",
      "year": "1999",
      "journal-title": "Science."
    },
    {
      "key": "2023072213555872700_BIB7",
      "doi-asserted-by": "crossref",
      "first-page": "13655",
      "DOI": "10.1016/S0021-9258(18)77399-7",
      "volume": "265",
      "year": "1990",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2023072213555872700_BIB8",
      "doi-asserted-by": "crossref",
      "first-page": "3549",
      "DOI": "10.1091/mbc.10.11.3549",
      "volume": "10",
      "year": "1999",
      "journal-title": "Mol. Biol. Cell."
    },
    {
      "key": "2023072213555872700_BIB9",
      "doi-asserted-by": "crossref",
      "first-page": "9",
      "DOI": "10.1016/S0962-8924(00)88926-6",
      "volume": "5",
      "year": "1995",
      "journal-title": "Trends Cell Biol."
    },
    {
      "key": "2023072213555872700_BIB10",
      "doi-asserted-by": "crossref",
      "first-page": "607",
      "DOI": "10.1046/j.1365-2958.1997.3771735.x",
      "volume": "24",
      "year": "1997",
      "journal-title": "Mol. Microbiol."
    },
    {
      "key": "2023072213555872700_BIB11",
      "doi-asserted-by": "crossref",
      "first-page": "187",
      "DOI": "10.1038/34438",
      "volume": "391",
      "year": "1998",
      "journal-title": "Nature."
    },
    {
      "key": "2023072213555872700_BIB12",
      "doi-asserted-by": "crossref",
      "first-page": "585",
      "DOI": "10.1083/jcb.135.3.585",
      "volume": "135",
      "year": "1996",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072213555872700_BIB13",
      "doi-asserted-by": "crossref",
      "first-page": "10704",
      "DOI": "10.1073/pnas.191359398",
      "volume": "98",
      "year": "2001",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072213555872700_BIB14",
      "doi-asserted-by": "crossref",
      "first-page": "316",
      "DOI": "10.1126/science.291.5502.316",
      "volume": "291",
      "year": "2001",
      "journal-title": "Science."
    },
    {
      "key": "2023072213555872700_BIB15",
      "doi-asserted-by": "crossref",
      "first-page": "81",
      "DOI": "10.1016/S0092-8674(00)80608-X",
      "volume": "98",
      "year": "1999",
      "journal-title": "Cell."
    },
    {
      "key": "2023072213555872700_BIB16",
      "doi-asserted-by": "crossref",
      "first-page": "13705",
      "DOI": "10.1073/pnas.241522198",
      "volume": "98",
      "year": "2001",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072213555872700_BIB17",
      "doi-asserted-by": "crossref",
      "first-page": "556",
      "DOI": "10.1126/science.277.5325.556",
      "volume": "277",
      "year": "1997",
      "journal-title": "Science."
    },
    {
      "key": "2023072213555872700_BIB18",
      "doi-asserted-by": "crossref",
      "first-page": "7227",
      "DOI": "10.1073/pnas.89.15.7227",
      "volume": "89",
      "year": "1992",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072213555872700_BIB19",
      "doi-asserted-by": "crossref",
      "first-page": "895",
      "DOI": "10.1083/jcb.135.4.895",
      "volume": "135",
      "year": "1996",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072213555872700_BIB20",
      "doi-asserted-by": "crossref",
      "first-page": "1526",
      "DOI": "10.1126/science.271.5255.1526",
      "volume": "271",
      "year": "1996",
      "journal-title": "Science."
    },
    {
      "key": "2023072213555872700_BIB21",
      "doi-asserted-by": "crossref",
      "first-page": "339",
      "DOI": "10.1007/BF00340712",
      "volume": "16",
      "year": "1989",
      "journal-title": "Curr. Genet."
    },
    {
      "key": "2023072213555872700_BIB22",
      "doi-asserted-by": "crossref",
      "first-page": "13",
      "DOI": "10.1091/mbc.11.1.13",
      "volume": "11",
      "year": "2000",
      "journal-title": "Mol. Biol. Cell."
    },
    {
      "key": "2023072213555872700_BIB23",
      "doi-asserted-by": "crossref",
      "first-page": "258",
      "DOI": "10.1016/S0076-6879(02)51852-8",
      "volume": "351",
      "year": "2002",
      "journal-title": "Methods Enzymol."
    },
    {
      "key": "2023072213555872700_BIB24",
      "doi-asserted-by": "crossref",
      "first-page": "13643",
      "DOI": "10.1073/pnas.250472397",
      "volume": "97",
      "year": "2000",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072213555872700_BIB25",
      "doi-asserted-by": "crossref",
      "first-page": "6742",
      "DOI": "10.1093/emboj/20.23.6742",
      "volume": "20",
      "year": "2001",
      "journal-title": "EMBO J."
    },
    {
      "key": "2023072213555872700_BIB26",
      "doi-asserted-by": "crossref",
      "first-page": "289",
      "DOI": "10.1016/0092-8674(87)90224-8",
      "volume": "50",
      "year": "1987",
      "journal-title": "Cell."
    },
    {
      "key": "2023072213555872700_BIB27",
      "doi-asserted-by": "crossref",
      "first-page": "124",
      "DOI": "10.1016/0076-6879(92)19015-X",
      "volume": "219",
      "year": "1992",
      "journal-title": "Methods Enzymol."
    },
    {
      "key": "2023072213555872700_BIB28",
      "doi-asserted-by": "crossref",
      "first-page": "30567",
      "DOI": "10.1074/jbc.270.51.30567",
      "volume": "270",
      "year": "1995",
      "journal-title": "J. Biol. Chem."
    }
  ],
  "container-title": "The Journal of Cell Biology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://rupress.org/jcb/article-pdf/159/6/915/1520663/jcb1596915.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://rupress.org/jcb/article-pdf/159/6/915/1520663/jcb1596915.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        7,
        22
      ]
    ],
    "date-time": "2023-07-22T13:56:22Z",
    "timestamp": 1690034182000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://rupress.org/jcb/article/159/6/915/33274/Concentrative-sorting-of-secretory-cargo-proteins"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2002,
        12,
        23
      ]
    ]
  },
  "references-count": 28,
  "journal-issue": {
    "issue": "6",
    "published-print": {
      "date-parts": [
        [
          2002,
          12,
          23
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1083/jcb.200208074",
  "relation": {
    "has-review": [
      {
        "id-type": "doi",
        "id": "10.3410/f.1007641.180863",
        "asserted-by": "object"
      }
    ]
  },
  "ISSN": [
    "1540-8140",
    "0021-9525"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        2002,
        12,
        23
      ]
    ]
  }
}