Crossref journal-article
Rockefeller University Press
The Journal of Cell Biology (291)
Abstract

Two homologous GTP-binding proteins, atToc33 and atToc159, control access of cytosolic precursor proteins to the chloroplast. atToc33 is a constitutive outer chloroplast membrane protein, whereas the precursor receptor atToc159 also exists in a soluble, cytosolic form. This suggests that atToc159 may be able to switch between a soluble and an integral membrane form. By transient expression of GFP fusion proteins, mutant analysis, and biochemical experimentation, we demonstrate that the GTP-binding domain regulates the targeting of cytosolic atToc159 to the chloroplast and mediates the switch between cytosolic and integral membrane forms. Mutant atToc159, unable to bind GTP, does not reinstate a green phenotype in an albino mutant (ppi2) lacking endogenous atToc159, remaining trapped in the cytosol. Thus, the function of atToc159 in chloroplast biogenesis is dependent on an intrinsic GTP-regulated switch that controls localization of the receptor to the chloroplast envelope.

Bibliography

Bauer, J., Hiltbrunner, A., Weibel, P., Vidi, P.-A., Alvarez-Huerta, M., Smith, M. D., Schnell, D. J., & Kessler, F. (2002). Essential role of the G-domain in targeting of the protein import receptor atToc159 to the chloroplast outer membrane. The Journal of Cell Biology, 159(5), 845–854.

Authors 8
  1. Jörg Bauer (first)
  2. Andreas Hiltbrunner (additional)
  3. Petra Weibel (additional)
  4. Pierre-Alexandre Vidi (additional)
  5. Mayte Alvarez-Huerta (additional)
  6. Matthew D. Smith (additional)
  7. Danny J. Schnell (additional)
  8. Felix Kessler (additional)
References 36 Referenced 71
  1. 10.1038/35003214 / Nature. (2000)
  2. 10.1007/PL00000867 / Cell. Mol. Life Sci. (2001)
  3. {'key': '2023072213572036400_BIB3', 'first-page': '259', 'volume': '82', 'year': '1998', 'journal-title': 'Methods Mol. Biol.'} / Methods Mol. Biol. (1998)
  4. 10.1016/S0014-5793(98)01525-7 / FEBS Lett. (1998)
  5. 10.1074/jbc.272.10.6614 / J. Biol. Chem. (1997)
  6. 10.1104/pp.122.3.813 / Plant Physiol. (2000)
  7. 10.1042/bst0280485 / Biochem. Soc. Trans. (2000)
  8. 10.1038/35003082 / Nature. (2000)
  9. 10.1083/jcb.123.4.799 / J. Cell Biol. (1993)
  10. 10.1046/j.0960-7412.2001.01061.x / Plant J. (2001)
  11. 10.1016/0378-1119(83)90230-5 / Gene. (1983)
  12. 10.1139/o00-088 / Biochem. Cell Biol. (2001)
  13. 10.1083/jcb.200104022 / J. Cell Biol. (2001)
  14. 10.1093/emboj/16.24.7351 / EMBO J. (1997)
  15. 10.1126/science.7801125 / Science. (1994)
  16. 10.1104/pp.125.4.1567 / Plant Physiol. (2001)
  17. 10.1126/science.282.5386.100 / Science. (1998)
  18. 10.1105/TPC.000534 / Plant Cell. (2001)
  19. 10.1105/tpc.11.4.557 / Plant Cell. (1999)
  20. 10.1038/nsb0202-81 / Nat. Struct. Biol. (2002)
  21. 10.1126/science.7973656 / Science. (1994)
  22. 10.1042/bj3460501 / Biochem. J. (2000)
  23. 10.1083/jcb.134.2.315 / J. Cell Biol. (1996)
  24. 10.1074/jbc.271.39.23846 / J. Biol. Chem. (1996)
  25. {'key': '2023072213572036400_BIB25', 'first-page': '93', 'volume': '6', 'year': '1994', 'journal-title': 'Plant Cell.'} / Plant Cell. (1994)
  26. 10.1104/pp.118.2.691 / Plant Physiol. (1998)
  27. 10.1007/s004250000357 / Planta. (2000)
  28. 10.1016/S0167-4889(01)00152-5 / Biochim. Biophys. Acta. (2001)
  29. 10.1021/bi011361+ / Biochemistry. (2002)
  30. 10.1126/science.7973649 / Science. (1994)
  31. 10.1083/jcb.200208017 / J. Cell Biol. (2002)
  32. 10.1038/nsb744 / Nat. Struct. Biol. (2002)
  33. 10.1073/pnas.080491597 / Proc. Natl. Acad. Sci. USA. (2000)
  34. 10.1002/j.1460-2075.1995.tb07364.x / EMBO J. (1995)
  35. {'key': '2023072213572036400_BIB35', 'first-page': '2093', 'volume': '8', 'year': '1996', 'journal-title': 'Plant Cell.'} / Plant Cell. (1996)
  36. 10.1016/0003-2697(84)90782-6 / Anal. Biochem. (1984)
Dates
Type When
Created 22 years, 8 months ago (Dec. 9, 2002, 7:53 p.m.)
Deposited 2 years, 1 month ago (July 22, 2023, 9:58 a.m.)
Indexed 2 weeks, 4 days ago (Aug. 5, 2025, 9 a.m.)
Issued 22 years, 8 months ago (Dec. 2, 2002)
Published 22 years, 8 months ago (Dec. 2, 2002)
Published Online 22 years, 8 months ago (Dec. 2, 2002)
Published Print 22 years, 8 months ago (Dec. 9, 2002)
Funders 0

None

@article{Bauer_2002, title={Essential role of the G-domain in targeting of the protein import receptor atToc159 to the chloroplast outer membrane}, volume={159}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200208018}, DOI={10.1083/jcb.200208018}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Bauer, Jörg and Hiltbrunner, Andreas and Weibel, Petra and Vidi, Pierre-Alexandre and Alvarez-Huerta, Mayte and Smith, Matthew D. and Schnell, Danny J. and Kessler, Felix}, year={2002}, month=dec, pages={845–854} }