Abstract
Two homologous GTP-binding proteins, atToc33 and atToc159, control access of cytosolic precursor proteins to the chloroplast. atToc33 is a constitutive outer chloroplast membrane protein, whereas the precursor receptor atToc159 also exists in a soluble, cytosolic form. This suggests that atToc159 may be able to switch between a soluble and an integral membrane form. By transient expression of GFP fusion proteins, mutant analysis, and biochemical experimentation, we demonstrate that the GTP-binding domain regulates the targeting of cytosolic atToc159 to the chloroplast and mediates the switch between cytosolic and integral membrane forms. Mutant atToc159, unable to bind GTP, does not reinstate a green phenotype in an albino mutant (ppi2) lacking endogenous atToc159, remaining trapped in the cytosol. Thus, the function of atToc159 in chloroplast biogenesis is dependent on an intrinsic GTP-regulated switch that controls localization of the receptor to the chloroplast envelope.
Bibliography
Bauer, J., Hiltbrunner, A., Weibel, P., Vidi, P.-A., Alvarez-Huerta, M., Smith, M. D., Schnell, D. J., & Kessler, F. (2002). Essential role of the G-domain in targeting of the protein import receptor atToc159 to the chloroplast outer membrane. The Journal of Cell Biology, 159(5), 845â854.
References
36
Referenced
71
10.1038/35003214
/ Nature. (2000)10.1007/PL00000867
/ Cell. Mol. Life Sci. (2001){'key': '2023072213572036400_BIB3', 'first-page': '259', 'volume': '82', 'year': '1998', 'journal-title': 'Methods Mol. Biol.'}
/ Methods Mol. Biol. (1998)10.1016/S0014-5793(98)01525-7
/ FEBS Lett. (1998)10.1074/jbc.272.10.6614
/ J. Biol. Chem. (1997)10.1104/pp.122.3.813
/ Plant Physiol. (2000)10.1042/bst0280485
/ Biochem. Soc. Trans. (2000)10.1038/35003082
/ Nature. (2000)10.1083/jcb.123.4.799
/ J. Cell Biol. (1993)10.1046/j.0960-7412.2001.01061.x
/ Plant J. (2001)10.1016/0378-1119(83)90230-5
/ Gene. (1983)10.1139/o00-088
/ Biochem. Cell Biol. (2001)10.1083/jcb.200104022
/ J. Cell Biol. (2001)10.1093/emboj/16.24.7351
/ EMBO J. (1997)10.1126/science.7801125
/ Science. (1994)10.1104/pp.125.4.1567
/ Plant Physiol. (2001)10.1126/science.282.5386.100
/ Science. (1998)10.1105/TPC.000534
/ Plant Cell. (2001)10.1105/tpc.11.4.557
/ Plant Cell. (1999)10.1038/nsb0202-81
/ Nat. Struct. Biol. (2002)10.1126/science.7973656
/ Science. (1994)10.1042/bj3460501
/ Biochem. J. (2000)10.1083/jcb.134.2.315
/ J. Cell Biol. (1996)10.1074/jbc.271.39.23846
/ J. Biol. Chem. (1996){'key': '2023072213572036400_BIB25', 'first-page': '93', 'volume': '6', 'year': '1994', 'journal-title': 'Plant Cell.'}
/ Plant Cell. (1994)10.1104/pp.118.2.691
/ Plant Physiol. (1998)10.1007/s004250000357
/ Planta. (2000)10.1016/S0167-4889(01)00152-5
/ Biochim. Biophys. Acta. (2001)10.1021/bi011361+
/ Biochemistry. (2002)10.1126/science.7973649
/ Science. (1994)10.1083/jcb.200208017
/ J. Cell Biol. (2002)10.1038/nsb744
/ Nat. Struct. Biol. (2002)10.1073/pnas.080491597
/ Proc. Natl. Acad. Sci. USA. (2000)10.1002/j.1460-2075.1995.tb07364.x
/ EMBO J. (1995){'key': '2023072213572036400_BIB35', 'first-page': '2093', 'volume': '8', 'year': '1996', 'journal-title': 'Plant Cell.'}
/ Plant Cell. (1996)10.1016/0003-2697(84)90782-6
/ Anal. Biochem. (1984)
Dates
Type | When |
---|---|
Created | 22 years, 8 months ago (Dec. 9, 2002, 7:53 p.m.) |
Deposited | 2 years, 1 month ago (July 22, 2023, 9:58 a.m.) |
Indexed | 2 weeks, 4 days ago (Aug. 5, 2025, 9 a.m.) |
Issued | 22 years, 8 months ago (Dec. 2, 2002) |
Published | 22 years, 8 months ago (Dec. 2, 2002) |
Published Online | 22 years, 8 months ago (Dec. 2, 2002) |
Published Print | 22 years, 8 months ago (Dec. 9, 2002) |
@article{Bauer_2002, title={Essential role of the G-domain in targeting of the protein import receptor atToc159 to the chloroplast outer membrane}, volume={159}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200208018}, DOI={10.1083/jcb.200208018}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Bauer, Jörg and Hiltbrunner, Andreas and Weibel, Petra and Vidi, Pierre-Alexandre and Alvarez-Huerta, Mayte and Smith, Matthew D. and Schnell, Danny J. and Kessler, Felix}, year={2002}, month=dec, pages={845–854} }