DOI: 10.1083/jcb.200204127. DNA replication is required for the checkpoint response to damaged DNA in <i>Xenopus</i> egg extracts

DNA replication is required for the checkpoint response to damaged DNA in Xenopus egg extracts

10.1083/jcb.200204127

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

Alkylating agents, such as methyl methanesulfonate (MMS), damage DNA and activate the DNA damage checkpoint. Although many of the checkpoint proteins that transduce damage signals have been identified and characterized, the mechanism that senses the damage and activates the checkpoint is not yet understood. To address this issue for alkylation damage, we have reconstituted the checkpoint response to MMS in Xenopus egg extracts. Using four different indicators for checkpoint activation (delay on entrance into mitosis, slowing of DNA replication, phosphorylation of the Chk1 protein, and physical association of the Rad17 checkpoint protein with damaged DNA), we report that MMS-induced checkpoint activation is dependent upon entrance into S phase. Additionally, we show that the replication of damaged double-stranded DNA, and not replication of damaged single-stranded DNA, is the molecular event that activates the checkpoint. Therefore, these data provide direct evidence that replication forks are an obligate intermediate in the activation of the DNA damage checkpoint.

Bibliography

Stokes, M. P., Van Hatten, R., Lindsay, H. D., & Michael, W. M. (2002). DNA replication is required for the checkpoint response to damaged DNA in Xenopus egg extracts. The Journal of Cell Biology, 158(5), 863â872.

Authors 4

Matthew P. Stokes (first)
Ruth Van Hatten (additional)
Howard D. Lindsay (additional)
W. Matthew Michael (additional)

References 44 Referenced 61

10.1101/gad.914401 / Genes Dev. (2001)
10.1038/35082110 / Nature. (2001)
10.1074/jbc.M001244200 / J. Biol. Chem. (2000)
10.1038/35003501 / Nature. (2000)
{'key': '2023072213443839200_BIB5'}
10.1016/S0960-9822(99)00263-8 / Curr. Biol. (2000)
10.1002/j.1460-2075.1995.tb00269.x / EMBO J. (1995)
10.1091/mbc.11.5.1535 / Mol. Biol. Cell. (2000)
10.1101/gad.842500 / Genes Dev. (2000)
10.1093/embo-reports/kvd076 / EMBO Rep. (2000)
10.1128/MCB.21.10.3289-3301.2001 / Mol. Cell. Biol. (2001)
10.1126/science.1063827 / Science. (2001)
10.1083/jcb.142.6.1559 / J. Cell Biol. (1998)
10.1016/S0092-8674(00)81482-8 / Cell. (1998)
10.1073/pnas.201373498 / Proc. Natl. Acad. Sci. USA. (2001)
10.1016/S0092-8674(00)81209-X / Cell. (1998)
10.1016/S0955-0674(02)00312-5 / Curr. Opin. Cell Biol. (2002)
10.1101/gad.903501 / Genes Dev. (2001)
10.1126/science.282.5395.1886 / Science. (1998)
10.1126/science.289.5487.2133 / Science. (2000)
10.1016/S0968-0004(00)01560-7 / Trends Biochem. Sci. (2000)
10.1016/S0091-679X(08)60298-8 / Methods Cell Biol. (1991)
10.1093/emboj/18.16.4485 / EMBO J. (1999)
10.1016/S0962-8924(00)01773-6 / Trends Cell Biol. (2000)
10.1016/S0027-5107(77)80031-6 / Mutat. Res. (1977)
10.1016/0092-8674(95)90481-6 / Cell. (1995)
10.1093/genetics/145.1.45 / Genetics. (1997)
10.1093/emboj/18.22.6561 / EMBO J. (1999)
10.1073/pnas.231364598 / Proc. Natl. Acad. Sci. USA. (2001)
{'key': '2023072213443839200_BIB30', 'first-page': '4375', 'volume': '59', 'year': '1999', 'journal-title': 'Cancer Res.'} / Cancer Res. (1999)
10.1091/mbc.10.12.3991 / Mol. Biol. Cell. (1999)
10.1128/MCB.18.9.5485 / Mol. Cell. Biol. (1998)
10.1038/27007 / Nature. (1998)
10.1073/pnas.90.17.7985 / Proc. Natl. Acad. Sci. USA. (1993)
10.1038/35055000 / Nat. Cell Biol. (2001)
10.1038/35087607 / Nature. (2001)
10.1016/0092-8674(94)90573-8 / Cell. (1994)
10.1093/emboj/17.19.5679 / EMBO J. (1998)
10.1016/S1097-2765(00)80052-0 / Mol. Cell. (1998)
{'key': '2023072213443839200_BIB40'}
10.1126/science.3291120 / Science. (1988)
10.1126/science.290.5500.2309 / Science. (2000)
10.1038/35044005 / Nature. (2000)
10.1101/gad.950302 / Genes Dev. (2002)

Dates

Type	When
Created	22 years, 11 months ago (Sept. 3, 2002, 3:13 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 9:45 a.m.)
Indexed	1 year ago (Aug. 4, 2024, 3:52 p.m.)
Issued	23 years ago (Sept. 2, 2002)
Published	23 years ago (Sept. 2, 2002)
Published Online	22 years, 11 months ago (Sept. 3, 2002)
Published Print	23 years ago (Sept. 2, 2002)

Funders 0

None

BibTeX

@article{Stokes_2002, title={DNA replication is required for the checkpoint response to damaged DNA in Xenopus egg extracts}, volume={158}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200204127}, DOI={10.1083/jcb.200204127}, number={5}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Stokes, Matthew P. and Van Hatten, Ruth and Lindsay, Howard D. and Michael, W. Matthew}, year={2002}, month=sep, pages={863–872} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        8,
        4
      ]
    ],
    "date-time": "2024-08-04T19:52:39Z",
    "timestamp": 1722801159943
  },
  "reference-count": 44,
  "publisher": "Rockefeller University Press",
  "issue": "5",
  "content-domain": {
    "domain": [
      "rupress.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2002,
        9,
        2
      ]
    ]
  },
  "abstract": "<jats:p>Alkylating agents, such as methyl methanesulfonate (MMS), damage DNA and activate the DNA damage checkpoint. Although many of the checkpoint proteins that transduce damage signals have been identified and characterized, the mechanism that senses the damage and activates the checkpoint is not yet understood. To address this issue for alkylation damage, we have reconstituted the checkpoint response to MMS in Xenopus egg extracts. Using four different indicators for checkpoint activation (delay on entrance into mitosis, slowing of DNA replication, phosphorylation of the Chk1 protein, and physical association of the Rad17 checkpoint protein with damaged DNA), we report that MMS-induced checkpoint activation is dependent upon entrance into S phase. Additionally, we show that the replication of damaged double-stranded DNA, and not replication of damaged single-stranded DNA, is the molecular event that activates the checkpoint. Therefore, these data provide direct evidence that replication forks are an obligate intermediate in the activation of the DNA damage checkpoint.</jats:p>",
  "DOI": "10.1083/jcb.200204127",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        9,
        3
      ]
    ],
    "date-time": "2002-09-03T19:13:39Z",
    "timestamp": 1031080419000
  },
  "page": "863-872",
  "update-policy": "http://dx.doi.org/10.1083/jcb.crossmarkpolicy",
  "source": "Crossref",
  "is-referenced-by-count": 61,
  "title": "DNA replication is required for the checkpoint response to damaged DNA in <i>Xenopus</i> egg extracts",
  "prefix": "10.1083",
  "volume": "158",
  "author": [
    {
      "given": "Matthew P.",
      "family": "Stokes",
      "sequence": "first",
      "affiliation": [
        {
          "name": "1The Biological Laboratories, Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138"
        }
      ]
    },
    {
      "given": "Ruth",
      "family": "Van Hatten",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "1The Biological Laboratories, Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138"
        }
      ]
    },
    {
      "given": "Howard D.",
      "family": "Lindsay",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "2Genome Damage and Stability Center, University of Sussex Falmer, Brighton BN1 9QG, UK"
        }
      ]
    },
    {
      "given": "W. Matthew",
      "family": "Michael",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "1The Biological Laboratories, Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138"
        }
      ]
    }
  ],
  "member": "291",
  "published-online": {
    "date-parts": [
      [
        2002,
        9,
        3
      ]
    ]
  },
  "reference": [
    {
      "key": "2023072213443839200_BIB1",
      "doi-asserted-by": "crossref",
      "first-page": "2177",
      "DOI": "10.1101/gad.914401",
      "volume": "15",
      "year": "2001",
      "journal-title": "Genes Dev."
    },
    {
      "key": "2023072213443839200_BIB2",
      "doi-asserted-by": "crossref",
      "first-page": "969",
      "DOI": "10.1038/35082110",
      "volume": "411",
      "year": "2001",
      "journal-title": "Nature."
    },
    {
      "key": "2023072213443839200_BIB3",
      "doi-asserted-by": "crossref",
      "first-page": "26343",
      "DOI": "10.1074/jbc.M001244200",
      "volume": "275",
      "year": "2000",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "2023072213443839200_BIB4",
      "doi-asserted-by": "crossref",
      "first-page": "37",
      "DOI": "10.1038/35003501",
      "volume": "404",
      "year": "2000",
      "journal-title": "Nature."
    },
    {
      "key": "2023072213443839200_BIB5"
    },
    {
      "key": "2023072213443839200_BIB6",
      "doi-asserted-by": "crossref",
      "first-page": "39",
      "DOI": "10.1016/S0960-9822(99)00263-8",
      "volume": "10",
      "year": "2000",
      "journal-title": "Curr. Biol."
    },
    {
      "key": "2023072213443839200_BIB7",
      "doi-asserted-by": "crossref",
      "first-page": "5812",
      "DOI": "10.1002/j.1460-2075.1995.tb00269.x",
      "volume": "14",
      "year": "1995",
      "journal-title": "EMBO J."
    },
    {
      "key": "2023072213443839200_BIB8",
      "doi-asserted-by": "crossref",
      "first-page": "1535",
      "DOI": "10.1091/mbc.11.5.1535",
      "volume": "11",
      "year": "2000",
      "journal-title": "Mol. Biol. Cell."
    },
    {
      "key": "2023072213443839200_BIB9",
      "doi-asserted-by": "crossref",
      "first-page": "2745",
      "DOI": "10.1101/gad.842500",
      "volume": "14",
      "year": "2000",
      "journal-title": "Genes Dev."
    },
    {
      "key": "2023072213443839200_BIB10",
      "doi-asserted-by": "crossref",
      "first-page": "319",
      "DOI": "10.1093/embo-reports/kvd076",
      "volume": "1",
      "year": "2000",
      "journal-title": "EMBO Rep."
    },
    {
      "key": "2023072213443839200_BIB11",
      "doi-asserted-by": "crossref",
      "first-page": "3289",
      "DOI": "10.1128/MCB.21.10.3289-3301.2001",
      "volume": "21",
      "year": "2001",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "2023072213443839200_BIB12",
      "doi-asserted-by": "crossref",
      "first-page": "867",
      "DOI": "10.1126/science.1063827",
      "volume": "294",
      "year": "2001",
      "journal-title": "Science."
    },
    {
      "key": "2023072213443839200_BIB13",
      "doi-asserted-by": "crossref",
      "first-page": "1559",
      "DOI": "10.1083/jcb.142.6.1559",
      "volume": "142",
      "year": "1998",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "2023072213443839200_BIB14",
      "doi-asserted-by": "crossref",
      "first-page": "399",
      "DOI": "10.1016/S0092-8674(00)81482-8",
      "volume": "94",
      "year": "1998",
      "journal-title": "Cell."
    },
    {
      "key": "2023072213443839200_BIB15",
      "doi-asserted-by": "crossref",
      "first-page": "11236",
      "DOI": "10.1073/pnas.201373498",
      "volume": "98",
      "year": "2001",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072213443839200_BIB16",
      "doi-asserted-by": "crossref",
      "first-page": "1043",
      "DOI": "10.1016/S0092-8674(00)81209-X",
      "volume": "93",
      "year": "1998",
      "journal-title": "Cell."
    },
    {
      "key": "2023072213443839200_BIB17",
      "doi-asserted-by": "crossref",
      "first-page": "237",
      "DOI": "10.1016/S0955-0674(02)00312-5",
      "volume": "14",
      "year": "2002",
      "journal-title": "Curr. Opin. Cell Biol."
    },
    {
      "key": "2023072213443839200_BIB18",
      "doi-asserted-by": "crossref",
      "first-page": "2809",
      "DOI": "10.1101/gad.903501",
      "volume": "15",
      "year": "2001",
      "journal-title": "Genes Dev."
    },
    {
      "key": "2023072213443839200_BIB19",
      "doi-asserted-by": "crossref",
      "first-page": "1886",
      "DOI": "10.1126/science.282.5395.1886",
      "volume": "282",
      "year": "1998",
      "journal-title": "Science."
    },
    {
      "key": "2023072213443839200_BIB20",
      "doi-asserted-by": "crossref",
      "first-page": "2133",
      "DOI": "10.1126/science.289.5487.2133",
      "volume": "289",
      "year": "2000",
      "journal-title": "Science."
    },
    {
      "key": "2023072213443839200_BIB21",
      "doi-asserted-by": "crossref",
      "first-page": "173",
      "DOI": "10.1016/S0968-0004(00)01560-7",
      "volume": "25",
      "year": "2000",
      "journal-title": "Trends Biochem. Sci."
    },
    {
      "key": "2023072213443839200_BIB22",
      "doi-asserted-by": "crossref",
      "first-page": "581",
      "DOI": "10.1016/S0091-679X(08)60298-8",
      "volume": "36",
      "year": "1991",
      "journal-title": "Methods Cell Biol."
    },
    {
      "key": "2023072213443839200_BIB23",
      "doi-asserted-by": "crossref",
      "first-page": "4485",
      "DOI": "10.1093/emboj/18.16.4485",
      "volume": "18",
      "year": "1999",
      "journal-title": "EMBO J."
    },
    {
      "key": "2023072213443839200_BIB24",
      "doi-asserted-by": "crossref",
      "first-page": "296",
      "DOI": "10.1016/S0962-8924(00)01773-6",
      "volume": "10",
      "year": "2000",
      "journal-title": "Trends Cell Biol."
    },
    {
      "key": "2023072213443839200_BIB25",
      "doi-asserted-by": "crossref",
      "first-page": "299",
      "DOI": "10.1016/S0027-5107(77)80031-6",
      "volume": "42",
      "year": "1977",
      "journal-title": "Mutat. Res."
    },
    {
      "key": "2023072213443839200_BIB26",
      "doi-asserted-by": "crossref",
      "first-page": "841",
      "DOI": "10.1016/0092-8674(95)90481-6",
      "volume": "82",
      "year": "1995",
      "journal-title": "Cell."
    },
    {
      "key": "2023072213443839200_BIB27",
      "doi-asserted-by": "crossref",
      "first-page": "45",
      "DOI": "10.1093/genetics/145.1.45",
      "volume": "145",
      "year": "1997",
      "journal-title": "Genetics."
    },
    {
      "key": "2023072213443839200_BIB28",
      "doi-asserted-by": "crossref",
      "first-page": "6561",
      "DOI": "10.1093/emboj/18.22.6561",
      "volume": "18",
      "year": "1999",
      "journal-title": "EMBO J."
    },
    {
      "key": "2023072213443839200_BIB29",
      "doi-asserted-by": "crossref",
      "first-page": "13102",
      "DOI": "10.1073/pnas.231364598",
      "volume": "98",
      "year": "2001",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072213443839200_BIB30",
      "first-page": "4375",
      "volume": "59",
      "year": "1999",
      "journal-title": "Cancer Res."
    },
    {
      "key": "2023072213443839200_BIB31",
      "doi-asserted-by": "crossref",
      "first-page": "3991",
      "DOI": "10.1091/mbc.10.12.3991",
      "volume": "10",
      "year": "1999",
      "journal-title": "Mol. Biol. Cell."
    },
    {
      "key": "2023072213443839200_BIB32",
      "doi-asserted-by": "crossref",
      "first-page": "5485",
      "DOI": "10.1128/MCB.18.9.5485",
      "volume": "18",
      "year": "1998",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "2023072213443839200_BIB33",
      "doi-asserted-by": "crossref",
      "first-page": "618",
      "DOI": "10.1038/27007",
      "volume": "395",
      "year": "1998",
      "journal-title": "Nature."
    },
    {
      "key": "2023072213443839200_BIB34",
      "doi-asserted-by": "crossref",
      "first-page": "7985",
      "DOI": "10.1073/pnas.90.17.7985",
      "volume": "90",
      "year": "1993",
      "journal-title": "Proc. Natl. Acad. Sci. USA."
    },
    {
      "key": "2023072213443839200_BIB35",
      "doi-asserted-by": "crossref",
      "first-page": "107",
      "DOI": "10.1038/35055000",
      "volume": "3",
      "year": "2001",
      "journal-title": "Nat. Cell Biol."
    },
    {
      "key": "2023072213443839200_BIB36",
      "doi-asserted-by": "crossref",
      "first-page": "553",
      "DOI": "10.1038/35087607",
      "volume": "412",
      "year": "2001",
      "journal-title": "Nature."
    },
    {
      "key": "2023072213443839200_BIB37",
      "doi-asserted-by": "crossref",
      "first-page": "67",
      "DOI": "10.1016/0092-8674(94)90573-8",
      "volume": "78",
      "year": "1994",
      "journal-title": "Cell."
    },
    {
      "key": "2023072213443839200_BIB38",
      "doi-asserted-by": "crossref",
      "first-page": "5679",
      "DOI": "10.1093/emboj/17.19.5679",
      "volume": "17",
      "year": "1998",
      "journal-title": "EMBO J."
    },
    {
      "key": "2023072213443839200_BIB39",
      "doi-asserted-by": "crossref",
      "first-page": "519",
      "DOI": "10.1016/S1097-2765(00)80052-0",
      "volume": "1",
      "year": "1998",
      "journal-title": "Mol. Cell."
    },
    {
      "key": "2023072213443839200_BIB40"
    },
    {
      "key": "2023072213443839200_BIB41",
      "doi-asserted-by": "crossref",
      "first-page": "317",
      "DOI": "10.1126/science.3291120",
      "volume": "241",
      "year": "1988",
      "journal-title": "Science."
    },
    {
      "key": "2023072213443839200_BIB42",
      "doi-asserted-by": "crossref",
      "first-page": "2309",
      "DOI": "10.1126/science.290.5500.2309",
      "volume": "290",
      "year": "2000",
      "journal-title": "Science."
    },
    {
      "key": "2023072213443839200_BIB43",
      "doi-asserted-by": "crossref",
      "first-page": "433",
      "DOI": "10.1038/35044005",
      "volume": "408",
      "year": "2000",
      "journal-title": "Nature."
    },
    {
      "key": "2023072213443839200_BIB44",
      "doi-asserted-by": "crossref",
      "first-page": "198",
      "DOI": "10.1101/gad.950302",
      "volume": "16",
      "year": "2002",
      "journal-title": "Genes Dev."
    }
  ],
  "container-title": "The Journal of Cell Biology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://rupress.org/jcb/article-pdf/158/5/863/1519091/jcb1585863.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://rupress.org/jcb/article-pdf/158/5/863/1519091/jcb1585863.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        7,
        22
      ]
    ],
    "date-time": "2023-07-22T13:45:02Z",
    "timestamp": 1690033502000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://rupress.org/jcb/article/158/5/863/32783/DNA-replication-is-required-for-the-checkpoint"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2002,
        9,
        2
      ]
    ]
  },
  "references-count": 44,
  "journal-issue": {
    "issue": "5",
    "published-print": {
      "date-parts": [
        [
          2002,
          9,
          2
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1083/jcb.200204127",
  "relation": {},
  "ISSN": [
    "1540-8140",
    "0021-9525"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        2002,
        9,
        2
      ]
    ]
  }
}