DOI: 10.1083/jcb.200112080. Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein

Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein–ubiquitin conjugates

10.1083/jcb.200112080

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

There is increasing evidence that ubiquitination of receptors provides an important endosomal sorting signal. Here we report that mammalian class E vacuolar protein-sorting (vps) proteins recognize ubiquitin. Both tumor susceptibility gene 101 (TSG101)/human VPS (hVPS)28 and hepatocyte growth factor receptor substrate (Hrs) cytosolic complexes bind ubiquitin-agarose. TSG101 and hVPS28 are localized to endosomes that contain internalized EGF receptor and label strongly for ubiquitinated proteins. Microinjection of anti-hVPS28 specifically retards EGF degradation and leads to endosomal accumulation of ubiquitin–protein conjugates. Likewise, depletion of TSG101 impairs EGF trafficking and causes dramatic relocalization of ubiquitin to endocytic compartments. Similar defects are found in cells overexpressing Hrs, further emphasizing the links between class E protein function, receptor trafficking, and endosomal ubiquitination.

Bibliography

Bishop, N., Horman, A., & Woodman, P. (2002). Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal proteinâubiquitin conjugates. The Journal of Cell Biology, 157(1), 91â102.

Authors 3

Naomi Bishop (first)
Alistair Horman (additional)
Philip Woodman (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 12:46 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 9:28 a.m.)
Indexed	5 days, 14 hours ago (Aug. 19, 2025, 6:06 a.m.)
Issued	23 years, 4 months ago (March 26, 2002)
Published	23 years, 4 months ago (March 26, 2002)
Published Online	23 years, 4 months ago (March 26, 2002)
Published Print	23 years, 4 months ago (April 1, 2002)

Funders 0

None

BibTeX

@article{Bishop_2002, title={Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein–ubiquitin conjugates}, volume={157}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200112080}, DOI={10.1083/jcb.200112080}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Bishop, Naomi and Horman, Alistair and Woodman, Philip}, year={2002}, month=mar, pages={91–102} }

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