DOI: 10.1083/jcb.200112047. Alteration of nuclear lamin organization inhibits RNA polymerase II

Alteration of nuclear lamin organization inhibits RNA polymerase II–dependent transcription

10.1083/jcb.200112047

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

RTegulation of gene activity is mediated by alterations in chromatin organization. In addition, chromatin organization may be governed in part by interactions with structural components of the nucleus. The nuclear lamins comprise the lamina and a variety of nucleoplasmic assemblies that together are major structural components of the nucleus. Furthermore, lamins and lamin-associated proteins have been reported to bind chromatin. These observations suggest that the nuclear lamins may be involved in the regulation of gene activity. In this report, we test this possibility by disrupting the normal organization of nuclear lamins with a dominant negative lamin mutant lacking the NH2-terminal domain. We find that this disruption inhibits RNA polymerase II activity in both mammalian cells and transcriptionally active embryonic nuclei from Xenopus laevis. The inhibition appears to be specific for polymerase II as disruption of lamin organization does not detectably inhibit RNA polymerases I and III. Furthermore, immunofluorescence observations indicate that this selective inhibition of polymerase II–dependent transcription involves the TATA binding protein, a component of the basal transcription factor TFIID.

Bibliography

Spann, T. P., Goldman, A. E., Wang, C., Huang, S., & Goldman, R. D. (2002). Alteration of nuclear lamin organization inhibits RNA polymerase IIâdependent transcription. The Journal of Cell Biology, 156(4), 603â608.

Authors 5

Timothy P. Spann (first)
Anne E. Goldman (additional)
Chen Wang (additional)
Sui Huang (additional)
Robert D. Goldman (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:46 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 9:27 a.m.)
Indexed	6 days, 3 hours ago (Aug. 28, 2025, 8:44 a.m.)
Issued	23 years, 6 months ago (Feb. 18, 2002)
Published	23 years, 6 months ago (Feb. 18, 2002)
Published Online	23 years, 6 months ago (Feb. 18, 2002)
Published Print	23 years, 6 months ago (Feb. 18, 2002)

Funders 0

None

BibTeX

@article{Spann_2002, title={Alteration of nuclear lamin organization inhibits RNA polymerase II–dependent transcription}, volume={156}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200112047}, DOI={10.1083/jcb.200112047}, number={4}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Spann, Timothy P. and Goldman, Anne E. and Wang, Chen and Huang, Sui and Goldman, Robert D.}, year={2002}, month=feb, pages={603–608} }

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