DOI: 10.1083/jcb.200110109. SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles

SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles

10.1083/jcb.200110109

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

RanGAP1 was the first documented substrate for conjugation with the ubiquitin-like protein SUMO-1. However, the functional significance of this conjugation has not been fully clarified. We sought to examine RanGAP1 behavior during mitosis. We found that RanGAP1 associates with mitotic spindles and that it is particularly concentrated at foci near kinetochores. Association with kinetochores appeared soon after nuclear envelope breakdown and persisted until late anaphase, but it was lost coincident with nuclear envelope assembly in telophase. A mutant RanGAP1 protein lacking the capacity to be conjugated to SUMO-1 no longer associated with spindles, indicating that conjugation was essential for RanGAP1's mitotic localization. RanBP2, a nuclear pore protein that binds SUMO-1–conjugated RanGAP1 during interphase, colocalized with RanGAP1 on spindles, suggesting that a complex between these two proteins may be involved in mitotic targeting of RanGAP1. This report shows for the first time that SUMO-1 conjugation is required for mitotic localization of RanGAP1, and suggests that a major role of SUMO-1 conjugation to RanGAP1 may be the spatial regulation of the Ran pathway during mitosis.

Bibliography

Joseph, J., Tan, S.-H., Karpova, T. S., McNally, J. G., & Dasso, M. (2002). SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles. The Journal of Cell Biology, 156(4), 595â602.

Authors 5

Jomon Joseph (first)
Shyh-Han Tan (additional)
Tatiana S. Karpova (additional)
James G. McNally (additional)
Mary Dasso (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:46 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 9:22 a.m.)
Indexed	6 days, 4 hours ago (Aug. 21, 2025, 1:32 p.m.)
Issued	23 years, 6 months ago (Feb. 18, 2002)
Published	23 years, 6 months ago (Feb. 18, 2002)
Published Online	23 years, 6 months ago (Feb. 18, 2002)
Published Print	23 years, 6 months ago (Feb. 18, 2002)

Funders 0

None

BibTeX

@article{Joseph_2002, title={SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles}, volume={156}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200110109}, DOI={10.1083/jcb.200110109}, number={4}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Joseph, Jomon and Tan, Shyh-Han and Karpova, Tatiana S. and McNally, James G. and Dasso, Mary}, year={2002}, month=feb, pages={595–602} }

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