DOI: 10.1083/jcb.200106061. Pivotal role of VASP in Arp2/3 complex–mediated actin nucleation, actin branch-formation, and <i>Listeria monocytogenes</i> motility

Pivotal role of VASP in Arp2/3 complex–mediated actin nucleation, actin branch-formation, and Listeria monocytogenes motility

10.1083/jcb.200106061

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

The Listeria monocytogenes ActA protein mediates actin-based motility by recruiting and stimulating the Arp2/3 complex. In vitro, the actin monomer-binding region of ActA is critical for stimulating Arp2/3-dependent actin nucleation; however, this region is dispensable for actin-based motility in cells. Here, we provide genetic and biochemical evidence that vasodilator-stimulated phosphoprotein (VASP) recruitment by ActA can bypass defects in actin monomer-binding. Furthermore, purified VASP enhances the actin-nucleating activity of wild-type ActA and the Arp2/3 complex while also reducing the frequency of actin branch formation. These data suggest that ActA stimulates the Arp2/3 complex by both VASP-dependent and -independent mechanisms that generate distinct populations of actin filaments in the comet tails of L. monocytogenes. The ability of VASP to contribute to actin filament nucleation and to regulate actin filament architecture highlights the central role of VASP in actin-based motility.

Bibliography

Skoble, J., Auerbuch, V., Goley, E. D., Welch, M. D., & Portnoy, D. A. (2001). Pivotal role of VASP in Arp2/3 complexâmediated actin nucleation, actin branch-formation, and Listeria monocytogenes motility. The Journal of Cell Biology, 155(1), 89â100.

Authors 5

Justin Skoble (first)
Victoria Auerbuch (additional)
Erin D. Goley (additional)
Matthew D. Welch (additional)
Daniel A. Portnoy (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:46 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 7:57 a.m.)
Indexed	2 months, 4 weeks ago (June 4, 2025, 6:05 a.m.)
Issued	23 years, 11 months ago (Oct. 1, 2001)
Published	23 years, 11 months ago (Oct. 1, 2001)
Published Online	23 years, 11 months ago (Oct. 1, 2001)
Published Print	23 years, 11 months ago (Oct. 1, 2001)

Funders 0

None

BibTeX

@article{Skoble_2001, title={Pivotal role of VASP in Arp2/3 complex–mediated actin nucleation, actin branch-formation, and Listeria monocytogenes motility}, volume={155}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200106061}, DOI={10.1083/jcb.200106061}, number={1}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Skoble, Justin and Auerbuch, Victoria and Goley, Erin D. and Welch, Matthew D. and Portnoy, Daniel A.}, year={2001}, month=oct, pages={89–100} }

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