DOI: 10.1083/jcb.200103010. Histamine treatment induces rearrangements of orthogonal arrays of particles (OAPs) in human AQP4-expressing gastric cells

Histamine treatment induces rearrangements of orthogonal arrays of particles (OAPs) in human AQP4-expressing gastric cells

10.1083/jcb.200103010

Crossref journal-article

Rockefeller University Press

The Journal of Cell Biology (291)

Abstract

To test the involvement of the water channel aquaporin (AQP)-4 in gastric acid physiology, the human gastric cell line (HGT)-1 was stably transfected with rat AQP4. AQP4 was immunolocalized to the basolateral membrane of transfected HGT-1 cells, like in native parietal cells. Expression of AQP4 in transfected cells increased the osmotic water permeability coefficient (Pf) from 2.02 ± 0.3 × 10−4 to 16.37 ± 0.5 × 10−4 cm/s at 20°C. Freeze-fracture EM showed distinct orthogonal arrays of particles (OAPs), the morphological signature of AQP4, on the plasma membrane of AQP4-expressing cells. Quantitative morphometry showed that the density of OAPs was 2.5 ± 0.3% under basal condition and decreased by 50% to 1.2 ± 0.3% after 20 min of histamine stimulation, mainly due to a significant decrease of the OAPs number. Concomitantly, Pf decreased by ∼35% in 20-min histamine-stimulated cells. Both Pf and OAPs density were not modified after 10 min of histamine exposure, time at which the maximal hormonal response is observed. Cell surface biotinylation experiments confirmed that AQP4 is internalized after 20 min of histamine exposure, which may account for the downregulation of water transport. This is the first evidence for short term rearrangement of OAPs in an established AQP4-expressing cell line.

Bibliography

Carmosino, M., Procino, G., Nicchia, G. P., Mannucci, R., Verbavatz, J.-M., Gobin, R., Svelto, M., & Valenti, G. (2001). Histamine treatment induces rearrangements of orthogonal arrays of particles (OAPs) in human AQP4-expressing gastric cells. The Journal of Cell Biology, 154(6), 1235â1244.

Authors 8

Monica Carmosino (first)
Giuseppe Procino (additional)
Grazia Paola Nicchia (additional)
Roberta Mannucci (additional)
Jean-Marc Verbavatz (additional)
Renèe Gobin (additional)
Maria Svelto (additional)
Giovanna Valenti (additional)

References 38 Referenced 34

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 12:46 p.m.)
Deposited	2 years, 1 month ago (July 22, 2023, 7:50 a.m.)
Indexed	4 weeks ago (July 30, 2025, 10:46 a.m.)
Issued	23 years, 11 months ago (Sept. 17, 2001)
Published	23 years, 11 months ago (Sept. 17, 2001)
Published Online	23 years, 11 months ago (Sept. 17, 2001)
Published Print	23 years, 11 months ago (Sept. 17, 2001)

Funders 0

None

BibTeX

@article{Carmosino_2001, title={Histamine treatment induces rearrangements of orthogonal arrays of particles (OAPs) in human AQP4-expressing gastric cells}, volume={154}, ISSN={0021-9525}, url={http://dx.doi.org/10.1083/jcb.200103010}, DOI={10.1083/jcb.200103010}, number={6}, journal={The Journal of Cell Biology}, publisher={Rockefeller University Press}, author={Carmosino, Monica and Procino, Giuseppe and Nicchia, Grazia Paola and Mannucci, Roberta and Verbavatz, Jean-Marc and Gobin, Renèe and Svelto, Maria and Valenti, Giovanna}, year={2001}, month=sep, pages={1235–1244} }

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  "journal-issue": {
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    "published-print": {
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  "URL": "http://dx.doi.org/10.1083/jcb.200103010",
  "relation": {},
  "ISSN": [
    "1540-8140",
    "0021-9525"
  ],
  "subject": [],
  "published": {
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        9,
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    ]
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}